File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Hepatocyte growth factor promotes in vitro scattering and morphogenesis of human cervical carcinoma cells

TitleHepatocyte growth factor promotes in vitro scattering and morphogenesis of human cervical carcinoma cells
Authors
Issue Date2000
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
Citation
Gynecologic Oncology, 2000, v. 78 n. 2, p. 158-165 How to Cite?
AbstractObjective. Hepatocyte growth factor (HGF) enhances cell dissociation and morphogenesis in many forms of carcinomas including some, but not all, cervical carcinomas. In this study, we examined the effects of HGF on two cervical cancer cell lines, derived from the same tumor, with different growth patterns in vivo and in vitro. Methods. Two cell lines, derived from the same cervical carcinoma, express spinous (C-4I) and basal (C4-II) squamous cell differentiation, respectively. A cell scattering assay was used to determine whether HGF would stimulate cell dissociation and motility. The morphogenetic capacity of HGF was assessed in collagen gel cultures, expression of the HGF receptor c-Met by Western blot analysis, and cadherin expression by immunofluorescence microscopy. Results. HGF-induced cell scattering was intense in C-4II, but limited in C-4I cultures. In Collagen gels, C-4I cells formed large, spherical compact colonies with necrotic centers while C-4II cells formed small, irregular colonies with no necrosis. HGF induced proliferation and branching morphogenesis in both lines, but more prominently in C-4II cultures. There was no difference in c-Met or E- and P-cadherin expression between C-4I and C-4II cultures, but the lines differed in their signal transduction responses to HGF. The scatter response was mediated primarily by phospha-tidylinositol 3-kinase in line, C-4I, but by mitogen-activated protein kinase in line C-4II. HGF induced collagen gel contraction by C-4 cells, demonstrating for the first time that HGF has the capacity to induce this function. Conclusions. The HGF-induced cell dispersion, morphogenesis, and collagen gel contraction in two cervical carcinoma cell lines were greatly influenced by differences between the lines in differentiation-associated properties. These properties, which include variations in extracellular matrix, junctional proteins, and signal transduction, may also modulate HGF action in vivo and thus determine patterns of invasiveness and growth of cervical carcinomas. (C) 2000 Academic Press.
Persistent Identifierhttp://hdl.handle.net/10722/178697
ISSN
2015 Impact Factor: 4.198
2015 SCImago Journal Rankings: 2.284
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, ASTen_US
dc.contributor.authorLeung, PCKen_US
dc.contributor.authorAuersperg, Nen_US
dc.date.accessioned2012-12-19T09:49:12Z-
dc.date.available2012-12-19T09:49:12Z-
dc.date.issued2000en_US
dc.identifier.citationGynecologic Oncology, 2000, v. 78 n. 2, p. 158-165en_US
dc.identifier.issn0090-8258en_US
dc.identifier.urihttp://hdl.handle.net/10722/178697-
dc.description.abstractObjective. Hepatocyte growth factor (HGF) enhances cell dissociation and morphogenesis in many forms of carcinomas including some, but not all, cervical carcinomas. In this study, we examined the effects of HGF on two cervical cancer cell lines, derived from the same tumor, with different growth patterns in vivo and in vitro. Methods. Two cell lines, derived from the same cervical carcinoma, express spinous (C-4I) and basal (C4-II) squamous cell differentiation, respectively. A cell scattering assay was used to determine whether HGF would stimulate cell dissociation and motility. The morphogenetic capacity of HGF was assessed in collagen gel cultures, expression of the HGF receptor c-Met by Western blot analysis, and cadherin expression by immunofluorescence microscopy. Results. HGF-induced cell scattering was intense in C-4II, but limited in C-4I cultures. In Collagen gels, C-4I cells formed large, spherical compact colonies with necrotic centers while C-4II cells formed small, irregular colonies with no necrosis. HGF induced proliferation and branching morphogenesis in both lines, but more prominently in C-4II cultures. There was no difference in c-Met or E- and P-cadherin expression between C-4I and C-4II cultures, but the lines differed in their signal transduction responses to HGF. The scatter response was mediated primarily by phospha-tidylinositol 3-kinase in line, C-4I, but by mitogen-activated protein kinase in line C-4II. HGF induced collagen gel contraction by C-4 cells, demonstrating for the first time that HGF has the capacity to induce this function. Conclusions. The HGF-induced cell dispersion, morphogenesis, and collagen gel contraction in two cervical carcinoma cell lines were greatly influenced by differences between the lines in differentiation-associated properties. These properties, which include variations in extracellular matrix, junctional proteins, and signal transduction, may also modulate HGF action in vivo and thus determine patterns of invasiveness and growth of cervical carcinomas. (C) 2000 Academic Press.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygynoen_US
dc.relation.ispartofGynecologic Oncologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCadherins - Biosynthesisen_US
dc.subject.meshCell Culture Techniquesen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Movement - Drug Effectsen_US
dc.subject.meshCollagenen_US
dc.subject.meshFemaleen_US
dc.subject.meshHepatocyte Growth Factor - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshKidney - Cytology - Drug Effectsen_US
dc.subject.meshMap Kinase Signaling System - Drug Effectsen_US
dc.subject.meshMicroscopy, Fluorescenceen_US
dc.subject.meshMorphogenesisen_US
dc.subject.meshProto-Oncogene Proteins C-Met - Biosynthesisen_US
dc.subject.meshTumor Cells, Cultured - Drug Effectsen_US
dc.subject.meshUterine Cervical Neoplasms - Metabolism - Pathologyen_US
dc.titleHepatocyte growth factor promotes in vitro scattering and morphogenesis of human cervical carcinoma cellsen_US
dc.typeArticleen_US
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_US
dc.identifier.authorityWong, AST=rp00805en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/gyno.2000.5877en_US
dc.identifier.pmid10926796-
dc.identifier.scopuseid_2-s2.0-0033849426en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033849426&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume78en_US
dc.identifier.issue2en_US
dc.identifier.spage158en_US
dc.identifier.epage165en_US
dc.identifier.isiWOS:000088812600013-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, AST=23987963300en_US
dc.identifier.scopusauthoridLeung, PCK=12782513900en_US
dc.identifier.scopusauthoridAuersperg, N=7006582556en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats