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Article: Methyl jasmonate induces expression of a novel Brassica juncea chitinase with two chitin-binding domains

TitleMethyl jasmonate induces expression of a novel Brassica juncea chitinase with two chitin-binding domains
Authors
Issue Date1999
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412
Citation
Plant Molecular Biology, 1999, v. 40 n. 6, p. 1009-1018 How to Cite?
AbstractWe have cloned a 1.3 kb Brassica juncea cDNA encoding BjCHI1, a novel acidic chitinase with two chitin-binding domains that shows 62% identity to Nicotiana tabacum Chia1 chitinase. BjCHI1 is structurally unlike Chia1 that has one chitin-binding domain, but resembles Chia5 chitinase UDA1, the precursor of Urtica dioica agglutinin; however there is only 36.9% identity between them. We propose that BjCHI1 should be classified under a new class, Chia7. The spacer and the hinge region of BjCHI1 are proline-rich, like that of Beta vulgaris Ch1, a Chia6 chitinase with half a chitin-binding domain. Northern blot analysis showed that the 1.3 kb BjCHI1 mRNA is induced by wounding and methyl jasmonate (MeJA) treatment but is unaffected by ethylene, salicylic acid (SA) or abscisic acid (ABA). This is the first report on MeJA induction of chitinase gene expression and further suggests that wound-related JA-mediated signal transduction is independent of that involving SA. Western blot analysis using polyclonal antibodies against BjCHI1 showed a cross-reacting band with an apparent molecular mass of 37 kDa in wounded tissues of B. juncea, revealing that, unlike UDA 1, BjCHI1 is not cleaved post-translationally at the hinge. Expression of recombinant BjCHI1 in Escherichia coli BL21 (DE3) inhibited its growth while crude extracts from E. coli JM109 expressing recombinant BjCHI1 showed chitinase activity. Results from polymerase chain reaction (PCR) suggest that genes encoding chitinases with single or double chitin-binding domains exist in B. juncea.
Persistent Identifierhttp://hdl.handle.net/10722/178677
ISSN
2015 Impact Factor: 3.905
2015 SCImago Journal Rankings: 1.915
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhao, KJen_US
dc.contributor.authorChye, MLen_US
dc.date.accessioned2012-12-19T09:49:05Z-
dc.date.available2012-12-19T09:49:05Z-
dc.date.issued1999en_US
dc.identifier.citationPlant Molecular Biology, 1999, v. 40 n. 6, p. 1009-1018en_US
dc.identifier.issn0167-4412en_US
dc.identifier.urihttp://hdl.handle.net/10722/178677-
dc.description.abstractWe have cloned a 1.3 kb Brassica juncea cDNA encoding BjCHI1, a novel acidic chitinase with two chitin-binding domains that shows 62% identity to Nicotiana tabacum Chia1 chitinase. BjCHI1 is structurally unlike Chia1 that has one chitin-binding domain, but resembles Chia5 chitinase UDA1, the precursor of Urtica dioica agglutinin; however there is only 36.9% identity between them. We propose that BjCHI1 should be classified under a new class, Chia7. The spacer and the hinge region of BjCHI1 are proline-rich, like that of Beta vulgaris Ch1, a Chia6 chitinase with half a chitin-binding domain. Northern blot analysis showed that the 1.3 kb BjCHI1 mRNA is induced by wounding and methyl jasmonate (MeJA) treatment but is unaffected by ethylene, salicylic acid (SA) or abscisic acid (ABA). This is the first report on MeJA induction of chitinase gene expression and further suggests that wound-related JA-mediated signal transduction is independent of that involving SA. Western blot analysis using polyclonal antibodies against BjCHI1 showed a cross-reacting band with an apparent molecular mass of 37 kDa in wounded tissues of B. juncea, revealing that, unlike UDA 1, BjCHI1 is not cleaved post-translationally at the hinge. Expression of recombinant BjCHI1 in Escherichia coli BL21 (DE3) inhibited its growth while crude extracts from E. coli JM109 expressing recombinant BjCHI1 showed chitinase activity. Results from polymerase chain reaction (PCR) suggest that genes encoding chitinases with single or double chitin-binding domains exist in B. juncea.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412en_US
dc.relation.ispartofPlant Molecular Biologyen_US
dc.subject.meshAcetic Acids - Pharmacologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshBrassica - Enzymology - Geneticsen_US
dc.subject.meshChitin - Metabolismen_US
dc.subject.meshChitinase - Genetics - Metabolismen_US
dc.subject.meshCyclopentanes - Pharmacologyen_US
dc.subject.meshDna, Complementary - Chemistry - Genetics - Isolation & Purificationen_US
dc.subject.meshDna, Plant - Geneticsen_US
dc.subject.meshEscherichia Coli - Genetics - Growth & Developmenten_US
dc.subject.meshGene Expression Regulation, Enzymologic - Drug Effectsen_US
dc.subject.meshGene Expression Regulation, Plant - Drug Effectsen_US
dc.subject.meshGenes, Plant - Geneticsen_US
dc.subject.meshGenome, Planten_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOxylipinsen_US
dc.subject.meshPlant Growth Regulators - Pharmacologyen_US
dc.subject.meshRecombinant Fusion Proteins - Genetics - Metabolismen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.titleMethyl jasmonate induces expression of a novel Brassica juncea chitinase with two chitin-binding domainsen_US
dc.typeArticleen_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1023/A:1006266407368en_US
dc.identifier.pmid10527425-
dc.identifier.scopuseid_2-s2.0-0033179935en_US
dc.identifier.hkuros48811-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033179935&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume40en_US
dc.identifier.issue6en_US
dc.identifier.spage1009en_US
dc.identifier.epage1018en_US
dc.identifier.isiWOS:000082567400011-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridZhao, KJ=7202071954en_US
dc.identifier.scopusauthoridChye, ML=7003905460en_US

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