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Article: The effect of polysaccharide krestin on nitric oxide production in mouse peritoneal macrophages

TitleThe effect of polysaccharide krestin on nitric oxide production in mouse peritoneal macrophages
Authors
KeywordsImmune Modulator
Interferon-Γ
Lipopolysaccharide
Macrophages
Nitric Oxide
Polysaccharide Krestin
Issue Date1999
PublisherChapman & Hall, Journals Department. The Journal's web site is located at http://www.chaphall.com/chaphall/journals.html
Citation
Medical Science Research, 1999, v. 27 n. 5, p. 299-302 How to Cite?
AbstractNitric oxide (NO) has an important role in preventing vascular thrombosis, inflammatory cell-mediated injury and reperfusion injury. It can inhibit several components of the atherogenic process. Polysaccharide krestin (PSK) is a protein-bound polysaccharide with proven effects on tumour therapy. Our previous work showed that PSK could protect macrophages from oxidative injury and prevent atherosclerosis. In order to determine the mechanisms of PSK action, we have now studied its effect on NO production in mouse peritoneal macrophages. The content of NO in cell cultures was analysed using Griess reagent, and the cell number determined by staining with crystal violet. There was no alteration in NO production by PSK-primed mouse peritoneal macrophages without any stimulation; but when the cells were stimulated with lipopolysaccharide (LPS), NO production increased significantly, Furthermore, NO production did not change when the PSK-primed macrophages were incubated with interferon-γ (IFN-γ). PSK may possibly exert its anti-atherogenic effects partly through regulating NO production in macrophages. The effect of PSK was similar to that of IFN-γ. The relationship between the effects of PSK and IFN-γ needs to be uncovered.
Persistent Identifierhttp://hdl.handle.net/10722/178672
ISSN
2001 Impact Factor: 0.384
2003 SCImago Journal Rankings: 0.107
References

 

DC FieldValueLanguage
dc.contributor.authorPang, ZJen_US
dc.contributor.authorChen, Yen_US
dc.contributor.authorZhou, Men_US
dc.contributor.authorWan, Jen_US
dc.date.accessioned2012-12-19T09:49:03Z-
dc.date.available2012-12-19T09:49:03Z-
dc.date.issued1999en_US
dc.identifier.citationMedical Science Research, 1999, v. 27 n. 5, p. 299-302en_US
dc.identifier.issn0269-8951en_US
dc.identifier.urihttp://hdl.handle.net/10722/178672-
dc.description.abstractNitric oxide (NO) has an important role in preventing vascular thrombosis, inflammatory cell-mediated injury and reperfusion injury. It can inhibit several components of the atherogenic process. Polysaccharide krestin (PSK) is a protein-bound polysaccharide with proven effects on tumour therapy. Our previous work showed that PSK could protect macrophages from oxidative injury and prevent atherosclerosis. In order to determine the mechanisms of PSK action, we have now studied its effect on NO production in mouse peritoneal macrophages. The content of NO in cell cultures was analysed using Griess reagent, and the cell number determined by staining with crystal violet. There was no alteration in NO production by PSK-primed mouse peritoneal macrophages without any stimulation; but when the cells were stimulated with lipopolysaccharide (LPS), NO production increased significantly, Furthermore, NO production did not change when the PSK-primed macrophages were incubated with interferon-γ (IFN-γ). PSK may possibly exert its anti-atherogenic effects partly through regulating NO production in macrophages. The effect of PSK was similar to that of IFN-γ. The relationship between the effects of PSK and IFN-γ needs to be uncovered.en_US
dc.languageengen_US
dc.publisherChapman & Hall, Journals Department. The Journal's web site is located at http://www.chaphall.com/chaphall/journals.htmlen_US
dc.relation.ispartofMedical Science Researchen_US
dc.subjectImmune Modulatoren_US
dc.subjectInterferon-Γen_US
dc.subjectLipopolysaccharideen_US
dc.subjectMacrophagesen_US
dc.subjectNitric Oxideen_US
dc.subjectPolysaccharide Krestinen_US
dc.titleThe effect of polysaccharide krestin on nitric oxide production in mouse peritoneal macrophagesen_US
dc.typeArticleen_US
dc.identifier.emailWan, J: jmfwan@hku.hken_US
dc.identifier.authorityWan, J=rp00798en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0033019919en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033019919&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume27en_US
dc.identifier.issue5en_US
dc.identifier.spage299en_US
dc.identifier.epage302en_US
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridPang, ZJ=7103343225en_US
dc.identifier.scopusauthoridChen, Y=16745998900en_US
dc.identifier.scopusauthoridZhou, M=7403506134en_US
dc.identifier.scopusauthoridWan, J=8930305000en_US

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