File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Constitutive and conditional cadherin expression in cultured human ovarian surface epithelium: Influence of family history of ovarian cancer

TitleConstitutive and conditional cadherin expression in cultured human ovarian surface epithelium: Influence of family history of ovarian cancer
Authors
Issue Date1999
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 1999, v. 81 n. 2, p. 180-188 How to Cite?
AbstractEpithelial ovarian carcinomas arise in a simple mesothelium (ovarian surface epithelium, OSE) but exhibit properties of oviductal and endometrial epithelia. Thus, during malignant progression, their differentiation proceeds from simple to complex, in contrast to carcinomas in other tissues. Related changes in OSE of women with a history of familial ovarian cancer indicate that this aberrant differentiation is initiated very early in neoplastic progression. The mechanisms underlying this process are not understood. Because cadherins are known regulators of differentiation, we investigated the relationship of the cadherins E, N and P to OSE morphology, growth patterns and differentiation in cultures of normal and metaplastic OSE from women with (FH-OSE) and without (NFH-OSE) a family history of ovarian cancer and in the ovarian carcinoma lines OVCAR-3 and CaOV3. We used immunofluorescence, RT-PCR, in situ hybridization and Western blotting. Our results define N-cadherin as the constitutively expressed cadherin of normal and metaplastic OSE and indicate that P-cadherin is undetectable while E- cadherin expression is conditional and related to genotype, stage of neoplastic progression and growth pattern. The altered expression of E- cadherin in apparently normal OSE of women with hereditary ovarian cancer syndromes in conjunction with the known capacity of E-cadherin to induce epithelial characteristics implicates this adhesion molecule as a possible inducer of the aberrant Mullerian differentiation which characterizes epithelial ovarian carcinomas. Abnormal differentiation in such (pre)- neoplastic tissues may represent an early, irreversible, non-mutational step in ovarian epithelial neoplastic progression.
Persistent Identifierhttp://hdl.handle.net/10722/178663
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
References

 

DC FieldValueLanguage
dc.contributor.authorWong, ASTen_US
dc.contributor.authorMainesBandiera, SLen_US
dc.contributor.authorRosen, Ben_US
dc.contributor.authorWheelock, MJen_US
dc.contributor.authorJohnson, KRen_US
dc.contributor.authorLeung, PCKen_US
dc.contributor.authorRoskelley, CDen_US
dc.contributor.authorAuersperg, Nen_US
dc.date.accessioned2012-12-19T09:49:01Z-
dc.date.available2012-12-19T09:49:01Z-
dc.date.issued1999en_US
dc.identifier.citationInternational Journal Of Cancer, 1999, v. 81 n. 2, p. 180-188en_US
dc.identifier.issn0020-7136en_US
dc.identifier.urihttp://hdl.handle.net/10722/178663-
dc.description.abstractEpithelial ovarian carcinomas arise in a simple mesothelium (ovarian surface epithelium, OSE) but exhibit properties of oviductal and endometrial epithelia. Thus, during malignant progression, their differentiation proceeds from simple to complex, in contrast to carcinomas in other tissues. Related changes in OSE of women with a history of familial ovarian cancer indicate that this aberrant differentiation is initiated very early in neoplastic progression. The mechanisms underlying this process are not understood. Because cadherins are known regulators of differentiation, we investigated the relationship of the cadherins E, N and P to OSE morphology, growth patterns and differentiation in cultures of normal and metaplastic OSE from women with (FH-OSE) and without (NFH-OSE) a family history of ovarian cancer and in the ovarian carcinoma lines OVCAR-3 and CaOV3. We used immunofluorescence, RT-PCR, in situ hybridization and Western blotting. Our results define N-cadherin as the constitutively expressed cadherin of normal and metaplastic OSE and indicate that P-cadherin is undetectable while E- cadherin expression is conditional and related to genotype, stage of neoplastic progression and growth pattern. The altered expression of E- cadherin in apparently normal OSE of women with hereditary ovarian cancer syndromes in conjunction with the known capacity of E-cadherin to induce epithelial characteristics implicates this adhesion molecule as a possible inducer of the aberrant Mullerian differentiation which characterizes epithelial ovarian carcinomas. Abnormal differentiation in such (pre)- neoplastic tissues may represent an early, irreversible, non-mutational step in ovarian epithelial neoplastic progression.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_US
dc.relation.ispartofInternational Journal of Canceren_US
dc.subject.meshAdulten_US
dc.subject.meshCadherins - Biosynthesis - Geneticsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCollagenen_US
dc.subject.meshDrug Combinationsen_US
dc.subject.meshEpithelial Cells - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshLamininen_US
dc.subject.meshMedical History Takingen_US
dc.subject.meshMicroscopy, Fluorescenceen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshOvarian Neoplasms - Genetics - Pathologyen_US
dc.subject.meshOvary - Cytology - Metabolismen_US
dc.subject.meshPolyhydroxyethyl Methacrylateen_US
dc.subject.meshProteoglycansen_US
dc.subject.meshRna, Messenger - Biosynthesisen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSurface Propertiesen_US
dc.titleConstitutive and conditional cadherin expression in cultured human ovarian surface epithelium: Influence of family history of ovarian canceren_US
dc.typeArticleen_US
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_US
dc.identifier.authorityWong, AST=rp00805en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/(SICI)1097-0215(19990412)81:2<180::AID-IJC3>3.0.CO;2-7en_US
dc.identifier.pmid10188716-
dc.identifier.scopuseid_2-s2.0-0032914810en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032914810&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume81en_US
dc.identifier.issue2en_US
dc.identifier.spage180en_US
dc.identifier.epage188en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, AST=23987963300en_US
dc.identifier.scopusauthoridMainesBandiera, SL=6602971815en_US
dc.identifier.scopusauthoridRosen, B=24290886900en_US
dc.identifier.scopusauthoridWheelock, MJ=7006781405en_US
dc.identifier.scopusauthoridJohnson, KR=7405967398en_US
dc.identifier.scopusauthoridLeung, PCK=12782513900en_US
dc.identifier.scopusauthoridRoskelley, CD=7004526388en_US
dc.identifier.scopusauthoridAuersperg, N=7006582556en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats