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Article: Differential expression of multiple cathepsin mRNAs in the rat testis during maturation and following lonidamine induced tissue restructuring

TitleDifferential expression of multiple cathepsin mRNAs in the rat testis during maturation and following lonidamine induced tissue restructuring
Authors
Issue Date1997
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/15216543.asp
Citation
Biochemistry And Molecular Biology International, 1997, v. 42 n. 2, p. 217-233 How to Cite?
AbstractIn the seminiferous epithelium, germ cell development behind the blood-testis barrier involves continual degradation and renewal of inter-testicular cell junctions. This allows: (i) the translocation of developing germ cells from the basal lamina to the adluminal compartment during spermatogenesis, and (ii) the eventual release of mature spermatids into the tubular lumen during spermiation. Throughout spermatogenesis, cellular debris must also be removed from the epithelium. Thus, it is conceivable that proteases, protease inhibitors, and cell junctional components are involved in these events. The present study sought to examine whether testicular cells can express multiple cathepsin mRNAs given that these proteases are involved in the degradation and processing of proteins as well as in tissue regeneration. By using total RNA isolated from primary cultures of Sertoli, Leydig, and germ cells for reverse-transcription and polymerase chain reaction (RT-PCR), the mRNAs of cathepsin B, C, D, H, L, and S were shown to be expressed by Sertoli and Leydig cells, whereas germ cells isolated from adult rats expressed all of the above cathepsin mRNAs except cathepsin D. Throughout postnatal development and maturation, the testicular steady-state mRNA levels of cathepsin B, C, D, L, and S remain relatively unchanged with the exception of cathepsin H whose mRNA level increased during maturation and peaked at 45-60 days of age. Using lonidamine, an anti-spermatogenic drug which is known to induce premature release of germ cells without affecting Leydig cell function by disrupting the inter-Sertoli-germ cell junctions, we have examined the differential expression of these cathepsin mRNAs in the testis at the time of extensive tissue restructuring. It was noted that the expression of cathepsin L and S in the testis increased significantly concomitant with the disappearance of elongate spermatids whereas the expression of cathepsin B, C, D, and H increased significantly when most of the round spermatids and spermatocytes were depleted. These results illustrate the intricate inter-relationship between these proteases in the testis during maturation and tissue restructuring.
Persistent Identifierhttp://hdl.handle.net/10722/178612
ISSN
2001 Impact Factor: 0.925
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMathur, PPen_US
dc.contributor.authorGrima, Jen_US
dc.contributor.authorMo, MYen_US
dc.contributor.authorZhu, LJen_US
dc.contributor.authorAravindan, GRen_US
dc.contributor.authorCalcagno, Ken_US
dc.contributor.authorO'bryan, Men_US
dc.contributor.authorChung, Sen_US
dc.contributor.authorMruk, Den_US
dc.contributor.authorLee, WMen_US
dc.contributor.authorSilvestrini, Ben_US
dc.contributor.authorCheng, CYen_US
dc.date.accessioned2012-12-19T09:48:42Z-
dc.date.available2012-12-19T09:48:42Z-
dc.date.issued1997en_US
dc.identifier.citationBiochemistry And Molecular Biology International, 1997, v. 42 n. 2, p. 217-233en_US
dc.identifier.issn1039-9712en_US
dc.identifier.urihttp://hdl.handle.net/10722/178612-
dc.description.abstractIn the seminiferous epithelium, germ cell development behind the blood-testis barrier involves continual degradation and renewal of inter-testicular cell junctions. This allows: (i) the translocation of developing germ cells from the basal lamina to the adluminal compartment during spermatogenesis, and (ii) the eventual release of mature spermatids into the tubular lumen during spermiation. Throughout spermatogenesis, cellular debris must also be removed from the epithelium. Thus, it is conceivable that proteases, protease inhibitors, and cell junctional components are involved in these events. The present study sought to examine whether testicular cells can express multiple cathepsin mRNAs given that these proteases are involved in the degradation and processing of proteins as well as in tissue regeneration. By using total RNA isolated from primary cultures of Sertoli, Leydig, and germ cells for reverse-transcription and polymerase chain reaction (RT-PCR), the mRNAs of cathepsin B, C, D, H, L, and S were shown to be expressed by Sertoli and Leydig cells, whereas germ cells isolated from adult rats expressed all of the above cathepsin mRNAs except cathepsin D. Throughout postnatal development and maturation, the testicular steady-state mRNA levels of cathepsin B, C, D, L, and S remain relatively unchanged with the exception of cathepsin H whose mRNA level increased during maturation and peaked at 45-60 days of age. Using lonidamine, an anti-spermatogenic drug which is known to induce premature release of germ cells without affecting Leydig cell function by disrupting the inter-Sertoli-germ cell junctions, we have examined the differential expression of these cathepsin mRNAs in the testis at the time of extensive tissue restructuring. It was noted that the expression of cathepsin L and S in the testis increased significantly concomitant with the disappearance of elongate spermatids whereas the expression of cathepsin B, C, D, and H increased significantly when most of the round spermatids and spermatocytes were depleted. These results illustrate the intricate inter-relationship between these proteases in the testis during maturation and tissue restructuring.en_US
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/15216543.aspen_US
dc.relation.ispartofBiochemistry and Molecular Biology Internationalen_US
dc.rightsBiochemistry and Molecular Biology International. Copyright © Informa Healthcare.-
dc.subject.meshAge Factorsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntispermatogenic Agents - Pharmacologyen_US
dc.subject.meshCathepsin Len_US
dc.subject.meshCathepsins - Drug Effects - Genetics - Metabolismen_US
dc.subject.meshCysteine Endopeptidasesen_US
dc.subject.meshEndopeptidasesen_US
dc.subject.meshEpithelium - Metabolismen_US
dc.subject.meshGene Expression Regulation, Developmental - Drug Effectsen_US
dc.subject.meshIndazoles - Pharmacologyen_US
dc.subject.meshLeydig Cells - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSeminiferous Tubules - Drug Effectsen_US
dc.subject.meshSertoli Cells - Metabolismen_US
dc.subject.meshTestis - Drug Effects - Growth & Development - Metabolismen_US
dc.titleDifferential expression of multiple cathepsin mRNAs in the rat testis during maturation and following lonidamine induced tissue restructuringen_US
dc.typeArticleen_US
dc.identifier.emailLee, WM: hrszlwm@hku.hken_US
dc.identifier.authorityLee, WM=rp00728en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9238520-
dc.identifier.scopuseid_2-s2.0-0031394952en_US
dc.identifier.hkuros22392-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031394952&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume42en_US
dc.identifier.issue2en_US
dc.identifier.spage217en_US
dc.identifier.epage233en_US
dc.identifier.isiWOS:000072915000001-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridMathur, PP=7201845754en_US
dc.identifier.scopusauthoridGrima, J=7003383792en_US
dc.identifier.scopusauthoridMo, MY=7006857340en_US
dc.identifier.scopusauthoridZhu, LJ=7404201524en_US
dc.identifier.scopusauthoridAravindan, GR=6602336336en_US
dc.identifier.scopusauthoridCalcagno, K=6507137028en_US
dc.identifier.scopusauthoridO'Bryan, M=7003581441en_US
dc.identifier.scopusauthoridChung, S=35104555300en_US
dc.identifier.scopusauthoridMruk, D=6701823934en_US
dc.identifier.scopusauthoridLee, WM=24799156600en_US
dc.identifier.scopusauthoridSilvestrini, B=7006825900en_US
dc.identifier.scopusauthoridCheng, CY=7404797787en_US

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