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Article: Determination of DMP 728, a IIb/IIIa receptor antagonist, in rat and dog plasma by high-performance liquid chromatography with fluorimetric detection

TitleDetermination of DMP 728, a IIb/IIIa receptor antagonist, in rat and dog plasma by high-performance liquid chromatography with fluorimetric detection
Authors
Issue Date1994
Citation
Journal Of Chromatography B: Biomedical Applications, 1994, v. 657 n. 1, p. 254-260 How to Cite?
AbstractA specific and sensitive HPLC assay for the determination of DMP 728 in dog and rat plasma has been developed. The method involves solid-phase extraction of DMP 728 and the internal standard from plasma using a C 2 column. The extracted compounds are derivatized with benzoin under alkaline conditions. Using a mixture of acetonitrile and 0.1 M potassium phosphate buffer (25:75, v/v, pH 7.4) as mobile phase, the derivatized products are separated on a Regis semipermeable surface C 8 column and monitored fluorometrically using 325 nm and 425 nm as excitation and emission wavelengths, respectively. The assay is linear from 2.5 to 1000 ng/ml in dog plasma and from 5 to 1000 ng/ml in rat plasma. The limit of quantitation is 2.5 ng/ml using 0.5 ml of dog plasma and 5 ng/ml using 0.5 ml of rat plasma. The assay has been used in pharmacokinetic studies of DMP 728 in dogs and rats.
Persistent Identifierhttp://hdl.handle.net/10722/178566
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWu, STen_US
dc.contributor.authorStampfli, HFen_US
dc.contributor.authorBanks, CMen_US
dc.contributor.authorEmm, TAen_US
dc.contributor.authorKapil, RPen_US
dc.contributor.authorPadovani, PKen_US
dc.contributor.authorLee Jr, WMen_US
dc.contributor.authorHuang, SMen_US
dc.date.accessioned2012-12-19T09:48:25Z-
dc.date.available2012-12-19T09:48:25Z-
dc.date.issued1994en_US
dc.identifier.citationJournal Of Chromatography B: Biomedical Applications, 1994, v. 657 n. 1, p. 254-260en_US
dc.identifier.issn1572-6495en_US
dc.identifier.urihttp://hdl.handle.net/10722/178566-
dc.description.abstractA specific and sensitive HPLC assay for the determination of DMP 728 in dog and rat plasma has been developed. The method involves solid-phase extraction of DMP 728 and the internal standard from plasma using a C 2 column. The extracted compounds are derivatized with benzoin under alkaline conditions. Using a mixture of acetonitrile and 0.1 M potassium phosphate buffer (25:75, v/v, pH 7.4) as mobile phase, the derivatized products are separated on a Regis semipermeable surface C 8 column and monitored fluorometrically using 325 nm and 425 nm as excitation and emission wavelengths, respectively. The assay is linear from 2.5 to 1000 ng/ml in dog plasma and from 5 to 1000 ng/ml in rat plasma. The limit of quantitation is 2.5 ng/ml using 0.5 ml of dog plasma and 5 ng/ml using 0.5 ml of rat plasma. The assay has been used in pharmacokinetic studies of DMP 728 in dogs and rats.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Chromatography B: Biomedical Applicationsen_US
dc.subject.meshAcetonitrilesen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBenzoinen_US
dc.subject.meshChromatography, High Pressure Liquid - Methods - Statistics & Numerical Dataen_US
dc.subject.meshDogsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshHydroxidesen_US
dc.subject.meshMesylates - Blood - Pharmacokineticsen_US
dc.subject.meshPeptides, Cyclic - Blood - Pharmacokineticsen_US
dc.subject.meshPhosphatesen_US
dc.subject.meshPlatelet Aggregation Inhibitors - Blood - Pharmacokineticsen_US
dc.subject.meshPlatelet Membrane Glycoproteins - Antagonists & Inhibitorsen_US
dc.subject.meshPotassium Compoundsen_US
dc.subject.meshRatsen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshTemperatureen_US
dc.subject.meshTime Factorsen_US
dc.titleDetermination of DMP 728, a IIb/IIIa receptor antagonist, in rat and dog plasma by high-performance liquid chromatography with fluorimetric detectionen_US
dc.typeArticleen_US
dc.identifier.emailLee Jr, WM: hrszlwm@hku.hken_US
dc.identifier.authorityLee Jr, WM=rp00728en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0378-4347(94)80097-9en_US
dc.identifier.pmid7952079-
dc.identifier.scopuseid_2-s2.0-0028300461en_US
dc.identifier.volume657en_US
dc.identifier.issue1en_US
dc.identifier.spage254en_US
dc.identifier.epage260en_US
dc.identifier.isiWOS:A1994NW84600036-
dc.identifier.scopusauthoridWu, ST=24297427300en_US
dc.identifier.scopusauthoridStampfli, HF=6701808971en_US
dc.identifier.scopusauthoridBanks, CM=7103312413en_US
dc.identifier.scopusauthoridEmm, TA=6603208673en_US
dc.identifier.scopusauthoridKapil, RP=7005157520en_US
dc.identifier.scopusauthoridPadovani, PK=7006090739en_US
dc.identifier.scopusauthoridLee Jr, WM=24799156600en_US
dc.identifier.scopusauthoridHuang, SM=14628613700en_US

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