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- Scopus: eid_2-s2.0-0027317333
- PMID: 8368266
- WOS: WOS:A1993LW03100008
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Article: Evaluation of antitumor effect of tumor necrosis factor in terms of protein metabolism and cell cycle kinetics
Title | Evaluation of antitumor effect of tumor necrosis factor in terms of protein metabolism and cell cycle kinetics |
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Authors | |
Keywords | bromodeoxyuridine cytokines flow cytometry protein breakdown protein synthesis tumor growth |
Issue Date | 1993 |
Publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ |
Citation | American Journal Of Physiology - Cell Physiology, 1993, v. 265 n. 2 34-2, p. C365-C374 How to Cite? |
Abstract | To determine the significance of protein breakdown in regulating tumor growth and to better understand the antitumor mechanism of tumor necrosis factor in vivo, we measured the effects of a 6-h constant intravenous infusion of human recombinant tumor necrosis factor-α (rHuTNF) on tumor protein metabolism and cell cycle kinetics in rats bearing the Walker-256 carcinosarcoma. Protein metabolism was investigated with the use of [14C]leucine infusion; estimates of tumor cell cycle kinetics were obtained in vivo by use of 5-bromo-2'-deoxyuridine (BrdUrd) pulse labeling and bivariate BrdUrd/DNA analysis by flow cytometry. Reduction in tumor growth by rHuTNF was associated with a dose-dependent increase in tumor proteolysis but no change in tumor protein synthesis. At the cellular level, rHuTNF had a significant cytostatic effect on G2/M cells and caused a marked decrease in the fraction of cells capable of BrdUrd uptake. Release of BrdUrd, an indicator of cell death, was noted in only 7.5% of tumor cells labeled at the beginning of rHuTNF infusion. These results suggest that either tumor protein breakdown may influence cell cycle activity by regulating cytoplasmic protein mass or that tumor proteolysis may be a compensatory mechanism for limiting cytoplasmic size when cellular division is interrupted suddenly. |
Persistent Identifier | http://hdl.handle.net/10722/178546 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wan, JM | en_US |
dc.contributor.author | Fogt, F | en_US |
dc.contributor.author | Bistrian, BR | en_US |
dc.contributor.author | Istfan, NW | en_US |
dc.date.accessioned | 2012-12-19T09:48:18Z | - |
dc.date.available | 2012-12-19T09:48:18Z | - |
dc.date.issued | 1993 | en_US |
dc.identifier.citation | American Journal Of Physiology - Cell Physiology, 1993, v. 265 n. 2 34-2, p. C365-C374 | en_US |
dc.identifier.issn | 0002-9513 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178546 | - |
dc.description.abstract | To determine the significance of protein breakdown in regulating tumor growth and to better understand the antitumor mechanism of tumor necrosis factor in vivo, we measured the effects of a 6-h constant intravenous infusion of human recombinant tumor necrosis factor-α (rHuTNF) on tumor protein metabolism and cell cycle kinetics in rats bearing the Walker-256 carcinosarcoma. Protein metabolism was investigated with the use of [14C]leucine infusion; estimates of tumor cell cycle kinetics were obtained in vivo by use of 5-bromo-2'-deoxyuridine (BrdUrd) pulse labeling and bivariate BrdUrd/DNA analysis by flow cytometry. Reduction in tumor growth by rHuTNF was associated with a dose-dependent increase in tumor proteolysis but no change in tumor protein synthesis. At the cellular level, rHuTNF had a significant cytostatic effect on G2/M cells and caused a marked decrease in the fraction of cells capable of BrdUrd uptake. Release of BrdUrd, an indicator of cell death, was noted in only 7.5% of tumor cells labeled at the beginning of rHuTNF infusion. These results suggest that either tumor protein breakdown may influence cell cycle activity by regulating cytoplasmic protein mass or that tumor proteolysis may be a compensatory mechanism for limiting cytoplasmic size when cellular division is interrupted suddenly. | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Cell Physiology | en_US |
dc.subject | bromodeoxyuridine | - |
dc.subject | cytokines | - |
dc.subject | flow cytometry | - |
dc.subject | protein breakdown | - |
dc.subject | protein synthesis | - |
dc.subject | tumor growth | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antineoplastic Agents - Pharmacology | en_US |
dc.subject.mesh | Bromodeoxyuridine - Pharmacokinetics | en_US |
dc.subject.mesh | Carcinosarcoma - Metabolism - Pathology | en_US |
dc.subject.mesh | Cell Cycle | en_US |
dc.subject.mesh | Cell Division | en_US |
dc.subject.mesh | Dna, Neoplasm - Metabolism | en_US |
dc.subject.mesh | Leucine - Metabolism | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Neoplasm Proteins - Metabolism | en_US |
dc.subject.mesh | Neoplasm Transplantation | en_US |
dc.subject.mesh | Peritoneal Neoplasms - Metabolism - Pathology | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Tumor Necrosis Factor-Alpha - Pharmacology | en_US |
dc.title | Evaluation of antitumor effect of tumor necrosis factor in terms of protein metabolism and cell cycle kinetics | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wan, JM: jmfwan@hku.hk | en_US |
dc.identifier.authority | Wan, JM=rp00798 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 8368266 | - |
dc.identifier.scopus | eid_2-s2.0-0027317333 | en_US |
dc.identifier.volume | 265 | en_US |
dc.identifier.issue | 2 34-2 | en_US |
dc.identifier.spage | C365 | en_US |
dc.identifier.epage | C374 | en_US |
dc.identifier.isi | WOS:A1993LW03100008 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Wan, JM=8930305000 | en_US |
dc.identifier.scopusauthorid | Fogt, F=7006446193 | en_US |
dc.identifier.scopusauthorid | Bistrian, BR=35463916700 | en_US |
dc.identifier.scopusauthorid | Istfan, NW=7003819779 | en_US |
dc.identifier.issnl | 0002-9513 | - |