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Article: Evaluation of antitumor effect of tumor necrosis factor in terms of protein metabolism and cell cycle kinetics

TitleEvaluation of antitumor effect of tumor necrosis factor in terms of protein metabolism and cell cycle kinetics
Authors
Issue Date1993
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Cell Physiology, 1993, v. 265 n. 2 34-2, p. C365-C374 How to Cite?
AbstractTo determine the significance of protein breakdown in regulating tumor growth and to better understand the antitumor mechanism of tumor necrosis factor in vivo, we measured the effects of a 6-h constant intravenous infusion of human recombinant tumor necrosis factor-α (rHuTNF) on tumor protein metabolism and cell cycle kinetics in rats bearing the Walker-256 carcinosarcoma. Protein metabolism was investigated with the use of [14C]leucine infusion; estimates of tumor cell cycle kinetics were obtained in vivo by use of 5-bromo-2'-deoxyuridine (BrdUrd) pulse labeling and bivariate BrdUrd/DNA analysis by flow cytometry. Reduction in tumor growth by rHuTNF was associated with a dose-dependent increase in tumor proteolysis but no change in tumor protein synthesis. At the cellular level, rHuTNF had a significant cytostatic effect on G2/M cells and caused a marked decrease in the fraction of cells capable of BrdUrd uptake. Release of BrdUrd, an indicator of cell death, was noted in only 7.5% of tumor cells labeled at the beginning of rHuTNF infusion. These results suggest that either tumor protein breakdown may influence cell cycle activity by regulating cytoplasmic protein mass or that tumor proteolysis may be a compensatory mechanism for limiting cytoplasmic size when cellular division is interrupted suddenly.
Persistent Identifierhttp://hdl.handle.net/10722/178546
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWan, JMen_US
dc.contributor.authorFogt, Fen_US
dc.contributor.authorBistrian, BRen_US
dc.contributor.authorIstfan, NWen_US
dc.date.accessioned2012-12-19T09:48:18Z-
dc.date.available2012-12-19T09:48:18Z-
dc.date.issued1993en_US
dc.identifier.citationAmerican Journal Of Physiology - Cell Physiology, 1993, v. 265 n. 2 34-2, p. C365-C374en_US
dc.identifier.issn0002-9513en_US
dc.identifier.urihttp://hdl.handle.net/10722/178546-
dc.description.abstractTo determine the significance of protein breakdown in regulating tumor growth and to better understand the antitumor mechanism of tumor necrosis factor in vivo, we measured the effects of a 6-h constant intravenous infusion of human recombinant tumor necrosis factor-α (rHuTNF) on tumor protein metabolism and cell cycle kinetics in rats bearing the Walker-256 carcinosarcoma. Protein metabolism was investigated with the use of [14C]leucine infusion; estimates of tumor cell cycle kinetics were obtained in vivo by use of 5-bromo-2'-deoxyuridine (BrdUrd) pulse labeling and bivariate BrdUrd/DNA analysis by flow cytometry. Reduction in tumor growth by rHuTNF was associated with a dose-dependent increase in tumor proteolysis but no change in tumor protein synthesis. At the cellular level, rHuTNF had a significant cytostatic effect on G2/M cells and caused a marked decrease in the fraction of cells capable of BrdUrd uptake. Release of BrdUrd, an indicator of cell death, was noted in only 7.5% of tumor cells labeled at the beginning of rHuTNF infusion. These results suggest that either tumor protein breakdown may influence cell cycle activity by regulating cytoplasmic protein mass or that tumor proteolysis may be a compensatory mechanism for limiting cytoplasmic size when cellular division is interrupted suddenly.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntineoplastic Agents - Pharmacologyen_US
dc.subject.meshBromodeoxyuridine - Pharmacokineticsen_US
dc.subject.meshCarcinosarcoma - Metabolism - Pathologyen_US
dc.subject.meshCell Cycleen_US
dc.subject.meshCell Divisionen_US
dc.subject.meshDna, Neoplasm - Metabolismen_US
dc.subject.meshLeucine - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshNeoplasm Proteins - Metabolismen_US
dc.subject.meshNeoplasm Transplantationen_US
dc.subject.meshPeritoneal Neoplasms - Metabolism - Pathologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshTumor Necrosis Factor-Alpha - Pharmacologyen_US
dc.titleEvaluation of antitumor effect of tumor necrosis factor in terms of protein metabolism and cell cycle kineticsen_US
dc.typeArticleen_US
dc.identifier.emailWan, JM: jmfwan@hku.hken_US
dc.identifier.authorityWan, JM=rp00798en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8368266-
dc.identifier.scopuseid_2-s2.0-0027317333en_US
dc.identifier.volume265en_US
dc.identifier.issue2 34-2en_US
dc.identifier.spageC365en_US
dc.identifier.epageC374en_US
dc.identifier.isiWOS:A1993LW03100008-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWan, JM=8930305000en_US
dc.identifier.scopusauthoridFogt, F=7006446193en_US
dc.identifier.scopusauthoridBistrian, BR=35463916700en_US
dc.identifier.scopusauthoridIstfan, NW=7003819779en_US

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