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Article: Characterization of D1 receptors mediating dopamine-stimulated growth hormone release from pituitary cells of the goldfish, Carassius auratus

TitleCharacterization of D1 receptors mediating dopamine-stimulated growth hormone release from pituitary cells of the goldfish, Carassius auratus
Authors
Issue Date1993
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 1993, v. 133 n. 2, p. 577-584 How to Cite?
AbstractPreviously, we demonstrated that dopamine (DA) stimulates GH release from the pituitary of goldfish, and this action is mediated by D1-like receptors. In the current study, we have provided evidence for the presence of D1- specific binding sites in the pituitary cells of goldfish. These D1-binding sites were found to be saturable, stereospecific, and selective for D1 ligands. The rank order of binding affinity of these D1-binding sites is (+)SCH23390 > SKF83566 >> (-)SCH23390 > domperidone > LY171555 >> serotonin. The association of these D1-binding sites with [3H]SCH23390, a D1-specific radioligand, was rapid, reversible, and exhibited a high binding affinity in the nanomolar range. The K(d) values were estimated to be 33.7 ± 8.5 nM for mixed populations of pituitary cells and 10.9 ± 2.5 nM for pituitary cell preparations enriched with somatotrophs. Autoradiographic studies revealed that specific binding of [3H]SCH23390 was predominantly localized in the pars distalis, not in the neurointermediate lobe of the goldfish pituitary. Furthermore, these D1-binding sites in the goldfish pituitary cells could be functionally correlated with the GH-releasing actions of DA. Since these D1- binding sites exhibited the expected pharmacological properties of mammalian D1 receptors, we conclude that DA D1 receptors are present in the goldfish pituitary and are responsible for the mediation of DA D1-stimulated GH release. The apparent similarities of the D1 receptor pharmacology between goldfish and mammals also suggests that DA D1 receptors are highly conserved during vertebrate evolution.
Persistent Identifierhttp://hdl.handle.net/10722/178545
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, AOLen_US
dc.contributor.authorChang, JPen_US
dc.contributor.authorPeter, REen_US
dc.date.accessioned2012-12-19T09:48:18Z-
dc.date.available2012-12-19T09:48:18Z-
dc.date.issued1993en_US
dc.identifier.citationEndocrinology, 1993, v. 133 n. 2, p. 577-584en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10722/178545-
dc.description.abstractPreviously, we demonstrated that dopamine (DA) stimulates GH release from the pituitary of goldfish, and this action is mediated by D1-like receptors. In the current study, we have provided evidence for the presence of D1- specific binding sites in the pituitary cells of goldfish. These D1-binding sites were found to be saturable, stereospecific, and selective for D1 ligands. The rank order of binding affinity of these D1-binding sites is (+)SCH23390 > SKF83566 >> (-)SCH23390 > domperidone > LY171555 >> serotonin. The association of these D1-binding sites with [3H]SCH23390, a D1-specific radioligand, was rapid, reversible, and exhibited a high binding affinity in the nanomolar range. The K(d) values were estimated to be 33.7 ± 8.5 nM for mixed populations of pituitary cells and 10.9 ± 2.5 nM for pituitary cell preparations enriched with somatotrophs. Autoradiographic studies revealed that specific binding of [3H]SCH23390 was predominantly localized in the pars distalis, not in the neurointermediate lobe of the goldfish pituitary. Furthermore, these D1-binding sites in the goldfish pituitary cells could be functionally correlated with the GH-releasing actions of DA. Since these D1- binding sites exhibited the expected pharmacological properties of mammalian D1 receptors, we conclude that DA D1 receptors are present in the goldfish pituitary and are responsible for the mediation of DA D1-stimulated GH release. The apparent similarities of the D1 receptor pharmacology between goldfish and mammals also suggests that DA D1 receptors are highly conserved during vertebrate evolution.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_US
dc.relation.ispartofEndocrinologyen_US
dc.subject.mesh2,3,4,5-Tetrahydro-7,8-Dihydroxy-1-Phenyl-1H-3-Benzazepine - Analogs & Derivatives - Metabolism - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshBenzazepines - Metabolismen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshDomperidone - Metabolismen_US
dc.subject.meshDopamine - Pharmacologyen_US
dc.subject.meshErgolines - Metabolismen_US
dc.subject.meshGoldfish - Physiologyen_US
dc.subject.meshGrowth Hormone - Secretionen_US
dc.subject.meshKineticsen_US
dc.subject.meshPituitary Gland - Drug Effects - Secretionen_US
dc.subject.meshQuinpiroleen_US
dc.subject.meshReceptors, Dopamine D1 - Physiologyen_US
dc.subject.meshSerotonin - Metabolismen_US
dc.subject.meshSomatostatin - Pharmacologyen_US
dc.titleCharacterization of D1 receptors mediating dopamine-stimulated growth hormone release from pituitary cells of the goldfish, Carassius auratusen_US
dc.typeArticleen_US
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_US
dc.identifier.authorityWong, AOL=rp00806en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/en.133.2.577en_US
dc.identifier.pmid8102094-
dc.identifier.scopuseid_2-s2.0-0027275082en_US
dc.identifier.volume133en_US
dc.identifier.issue2en_US
dc.identifier.spage577en_US
dc.identifier.epage584en_US
dc.identifier.isiWOS:A1993LQ84600023-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, AOL=7403147570en_US
dc.identifier.scopusauthoridChang, JP=7601547649en_US
dc.identifier.scopusauthoridPeter, RE=7202909690en_US

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