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Article: Characterization of cDNA and genomic clones encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase from Hevea brasiliensis

TitleCharacterization of cDNA and genomic clones encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase from Hevea brasiliensis
Authors
KeywordsCis-1,4-Polyisoprene
Gene Sequence
Isoprenoid Biosynthesis
Latex
Laticifer
Natural Rubber
Issue Date1991
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412
Citation
Plant Molecular Biology, 1991, v. 16 n. 4, p. 567-577 How to Cite?
AbstractHevea brasiliensis is the major producer of natural rubber which is cis-1,4-polyisoprene. The enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) is involved in the biosynthesis of rubber and other plant products. We have used a hamster HMGR cDNA clone as a heterologous hybridization probe to isolate and characterize cDNA and genomic clones of HMGR from H. brasiliensis. Sequence analysis revealed that these clones fall into two different classes, HMGR1 and HMGR2. Comparison of the two classes shows 86% nucleotide sequence homology and 95% amino acid homology. The carboxy-termini of Hevea HMGRs are highly homologous to those of hamster, yeast and Arabidopsis HMGR. The amino-terminus of Hevea HMGR contains two potential membrane-spanning domains as in Arabidopsis HMGR while seven such domains are found in the HMGRs of other organisms. The apparent molecular mass of Hevea HMGR was estimated in western blot analysis to be 59 kDa. Northern blot analysis indicated that the HMGR1 transcript of 2.4 kb is more highly-expressed in laticifer than in leaf. Genomic Southern analysis using 3′-end cDNA probes indicates the presence of at least two HMGR genes in Hevea. © 1991 Kluwer Academic Publishers.
Persistent Identifierhttp://hdl.handle.net/10722/178516
ISSN
2015 Impact Factor: 3.905
2015 SCImago Journal Rankings: 1.915
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChye, MLen_US
dc.contributor.authorKush, Aen_US
dc.contributor.authorTan, CTen_US
dc.contributor.authorChua, NHen_US
dc.date.accessioned2012-12-19T09:48:10Z-
dc.date.available2012-12-19T09:48:10Z-
dc.date.issued1991en_US
dc.identifier.citationPlant Molecular Biology, 1991, v. 16 n. 4, p. 567-577en_US
dc.identifier.issn0167-4412en_US
dc.identifier.urihttp://hdl.handle.net/10722/178516-
dc.description.abstractHevea brasiliensis is the major producer of natural rubber which is cis-1,4-polyisoprene. The enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) is involved in the biosynthesis of rubber and other plant products. We have used a hamster HMGR cDNA clone as a heterologous hybridization probe to isolate and characterize cDNA and genomic clones of HMGR from H. brasiliensis. Sequence analysis revealed that these clones fall into two different classes, HMGR1 and HMGR2. Comparison of the two classes shows 86% nucleotide sequence homology and 95% amino acid homology. The carboxy-termini of Hevea HMGRs are highly homologous to those of hamster, yeast and Arabidopsis HMGR. The amino-terminus of Hevea HMGR contains two potential membrane-spanning domains as in Arabidopsis HMGR while seven such domains are found in the HMGRs of other organisms. The apparent molecular mass of Hevea HMGR was estimated in western blot analysis to be 59 kDa. Northern blot analysis indicated that the HMGR1 transcript of 2.4 kb is more highly-expressed in laticifer than in leaf. Genomic Southern analysis using 3′-end cDNA probes indicates the presence of at least two HMGR genes in Hevea. © 1991 Kluwer Academic Publishers.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412en_US
dc.relation.ispartofPlant Molecular Biologyen_US
dc.subjectCis-1,4-Polyisopreneen_US
dc.subjectGene Sequenceen_US
dc.subjectIsoprenoid Biosynthesisen_US
dc.subjectLatexen_US
dc.subjectLaticiferen_US
dc.subjectNatural Rubberen_US
dc.titleCharacterization of cDNA and genomic clones encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase from Hevea brasiliensisen_US
dc.typeArticleen_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF00023422en_US
dc.identifier.pmid1714317-
dc.identifier.scopuseid_2-s2.0-0026145813en_US
dc.identifier.volume16en_US
dc.identifier.issue4en_US
dc.identifier.spage567en_US
dc.identifier.epage577en_US
dc.identifier.isiWOS:A1991FK21100007-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChye, ML=7003905460en_US
dc.identifier.scopusauthoridKush, A=6603781579en_US
dc.identifier.scopusauthoridTan, CT=7402818170en_US
dc.identifier.scopusauthoridChua, NH=36863130400en_US

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