File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1093/nar/18.24.7331
- Scopus: eid_2-s2.0-0025648758
- PMID: 2175432
- WOS: WOS:A1990EQ47900016
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Phosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK gene
Title | Phosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK gene |
---|---|
Authors | |
Issue Date | 1990 |
Publisher | Oxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/ |
Citation | Nucleic Acids Research, 1990, v. 18 n. 24, p. 7331-7337 How to Cite? |
Abstract | Yeast repressor activator protein 1 (RAP1) binds in vitro to specific DNA sequences that are found in diverse genetic elements. Expression of the yeast phosphoglycerate kinase gene (PGK) requires the binding of RAP1 to the activator core sequence within the upstream activating sequence (UAS) of PGK. A DNA fragment Z + which contains the activator core sequence of the PGK(UAS) has been shown to bind RAP1. Here we report that phosphatase treatment of RAP1 affected its binding to the PGK(UAS) but that this depended on the nature of the sequence flanking the 5' end of the activator core sequence. When the sequence flanking the 5' end of the activator core sequence was different from the PGK RAP1-binding site, phosphatase treatment of RAP1 decreased its binding to the DNA. When the 5' end of the binding site was a match to the PGK RAP1-binding site dephosphorylation of RAP1 increased RAP1 binding to the DNA. These observations were reproduced when the minimal functional DNA-binding domain of the RAP1 protein was used, implicating a phosphorylation-dependent binding of RAP1. This is the first evidence for phosphorylation-dependent binding of RAP1. |
Persistent Identifier | http://hdl.handle.net/10722/178503 |
ISSN | 2023 Impact Factor: 16.6 2023 SCImago Journal Rankings: 7.048 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tsang, JSH | en_US |
dc.contributor.author | Henry, YAL | en_US |
dc.contributor.author | Chambers, A | en_US |
dc.contributor.author | Kingsman, AJ | en_US |
dc.contributor.author | Kingsman, SM | en_US |
dc.date.accessioned | 2012-12-19T09:48:04Z | - |
dc.date.available | 2012-12-19T09:48:04Z | - |
dc.date.issued | 1990 | en_US |
dc.identifier.citation | Nucleic Acids Research, 1990, v. 18 n. 24, p. 7331-7337 | en_US |
dc.identifier.issn | 0305-1048 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178503 | - |
dc.description.abstract | Yeast repressor activator protein 1 (RAP1) binds in vitro to specific DNA sequences that are found in diverse genetic elements. Expression of the yeast phosphoglycerate kinase gene (PGK) requires the binding of RAP1 to the activator core sequence within the upstream activating sequence (UAS) of PGK. A DNA fragment Z + which contains the activator core sequence of the PGK(UAS) has been shown to bind RAP1. Here we report that phosphatase treatment of RAP1 affected its binding to the PGK(UAS) but that this depended on the nature of the sequence flanking the 5' end of the activator core sequence. When the sequence flanking the 5' end of the activator core sequence was different from the PGK RAP1-binding site, phosphatase treatment of RAP1 decreased its binding to the DNA. When the 5' end of the binding site was a match to the PGK RAP1-binding site dephosphorylation of RAP1 increased RAP1 binding to the DNA. These observations were reproduced when the minimal functional DNA-binding domain of the RAP1 protein was used, implicating a phosphorylation-dependent binding of RAP1. This is the first evidence for phosphorylation-dependent binding of RAP1. | en_US |
dc.language | eng | en_US |
dc.publisher | Oxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/ | en_US |
dc.relation.ispartof | Nucleic Acids Research | en_US |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Binding Sites | en_US |
dc.subject.mesh | Dna, Fungal - Genetics - Metabolism | en_US |
dc.subject.mesh | Dna-Binding Proteins - Metabolism | en_US |
dc.subject.mesh | Fungal Proteins - Metabolism | en_US |
dc.subject.mesh | Gene Expression Regulation, Fungal | en_US |
dc.subject.mesh | Genes, Fungal | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Phosphoglycerate Kinase - Genetics - Metabolism | en_US |
dc.subject.mesh | Phosphoric Monoester Hydrolases - Metabolism | en_US |
dc.subject.mesh | Phosphorylation | en_US |
dc.subject.mesh | Regulatory Sequences, Nucleic Acid | en_US |
dc.subject.mesh | Saccharomyces Cerevisiae - Enzymology - Genetics | en_US |
dc.subject.mesh | Transcription Factors | en_US |
dc.title | Phosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK gene | en_US |
dc.type | Article | en_US |
dc.identifier.email | Tsang, JSH: jshtsang@hku.hk | en_US |
dc.identifier.authority | Tsang, JSH=rp00792 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1093/nar/18.24.7331 | - |
dc.identifier.pmid | 2175432 | - |
dc.identifier.pmcid | PMC332870 | - |
dc.identifier.scopus | eid_2-s2.0-0025648758 | en_US |
dc.identifier.volume | 18 | en_US |
dc.identifier.issue | 24 | en_US |
dc.identifier.spage | 7331 | en_US |
dc.identifier.epage | 7337 | en_US |
dc.identifier.isi | WOS:A1990EQ47900016 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Tsang, JSH=7102483508 | en_US |
dc.identifier.scopusauthorid | Henry, YAL=26642929300 | en_US |
dc.identifier.scopusauthorid | Chambers, A=35563879400 | en_US |
dc.identifier.scopusauthorid | Kingsman, AJ=7006672393 | en_US |
dc.identifier.scopusauthorid | Kingsman, SM=7004417560 | en_US |
dc.identifier.issnl | 0305-1048 | - |