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Article: Phosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK gene

TitlePhosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK gene
Authors
Issue Date1990
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 1990, v. 18 n. 24, p. 7331-7337 How to Cite?
AbstractYeast repressor activator protein 1 (RAP1) binds in vitro to specific DNA sequences that are found in diverse genetic elements. Expression of the yeast phosphoglycerate kinase gene (PGK) requires the binding of RAP1 to the activator core sequence within the upstream activating sequence (UAS) of PGK. A DNA fragment Z + which contains the activator core sequence of the PGK(UAS) has been shown to bind RAP1. Here we report that phosphatase treatment of RAP1 affected its binding to the PGK(UAS) but that this depended on the nature of the sequence flanking the 5' end of the activator core sequence. When the sequence flanking the 5' end of the activator core sequence was different from the PGK RAP1-binding site, phosphatase treatment of RAP1 decreased its binding to the DNA. When the 5' end of the binding site was a match to the PGK RAP1-binding site dephosphorylation of RAP1 increased RAP1 binding to the DNA. These observations were reproduced when the minimal functional DNA-binding domain of the RAP1 protein was used, implicating a phosphorylation-dependent binding of RAP1. This is the first evidence for phosphorylation-dependent binding of RAP1.
Persistent Identifierhttp://hdl.handle.net/10722/178503
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsang, JSHen_US
dc.contributor.authorHenry, YALen_US
dc.contributor.authorChambers, Aen_US
dc.contributor.authorKingsman, AJen_US
dc.contributor.authorKingsman, SMen_US
dc.date.accessioned2012-12-19T09:48:04Z-
dc.date.available2012-12-19T09:48:04Z-
dc.date.issued1990en_US
dc.identifier.citationNucleic Acids Research, 1990, v. 18 n. 24, p. 7331-7337en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://hdl.handle.net/10722/178503-
dc.description.abstractYeast repressor activator protein 1 (RAP1) binds in vitro to specific DNA sequences that are found in diverse genetic elements. Expression of the yeast phosphoglycerate kinase gene (PGK) requires the binding of RAP1 to the activator core sequence within the upstream activating sequence (UAS) of PGK. A DNA fragment Z + which contains the activator core sequence of the PGK(UAS) has been shown to bind RAP1. Here we report that phosphatase treatment of RAP1 affected its binding to the PGK(UAS) but that this depended on the nature of the sequence flanking the 5' end of the activator core sequence. When the sequence flanking the 5' end of the activator core sequence was different from the PGK RAP1-binding site, phosphatase treatment of RAP1 decreased its binding to the DNA. When the 5' end of the binding site was a match to the PGK RAP1-binding site dephosphorylation of RAP1 increased RAP1 binding to the DNA. These observations were reproduced when the minimal functional DNA-binding domain of the RAP1 protein was used, implicating a phosphorylation-dependent binding of RAP1. This is the first evidence for phosphorylation-dependent binding of RAP1.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshDna, Fungal - Genetics - Metabolismen_US
dc.subject.meshDna-Binding Proteins - Metabolismen_US
dc.subject.meshFungal Proteins - Metabolismen_US
dc.subject.meshGene Expression Regulation, Fungalen_US
dc.subject.meshGenes, Fungalen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPhosphoglycerate Kinase - Genetics - Metabolismen_US
dc.subject.meshPhosphoric Monoester Hydrolases - Metabolismen_US
dc.subject.meshPhosphorylationen_US
dc.subject.meshRegulatory Sequences, Nucleic Aciden_US
dc.subject.meshSaccharomyces Cerevisiae - Enzymology - Geneticsen_US
dc.subject.meshTranscription Factorsen_US
dc.titlePhosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK geneen_US
dc.typeArticleen_US
dc.identifier.emailTsang, JSH: jshtsang@hku.hken_US
dc.identifier.authorityTsang, JSH=rp00792en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/nar/18.24.7331-
dc.identifier.pmid2175432-
dc.identifier.scopuseid_2-s2.0-0025648758en_US
dc.identifier.volume18en_US
dc.identifier.issue24en_US
dc.identifier.spage7331en_US
dc.identifier.epage7337en_US
dc.identifier.isiWOS:A1990EQ47900016-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridTsang, JSH=7102483508en_US
dc.identifier.scopusauthoridHenry, YAL=26642929300en_US
dc.identifier.scopusauthoridChambers, A=35563879400en_US
dc.identifier.scopusauthoridKingsman, AJ=7006672393en_US
dc.identifier.scopusauthoridKingsman, SM=7004417560en_US

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