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Article: Characterisation of the DNA binding domain of the yeast RAP1 protein

TitleCharacterisation of the DNA binding domain of the yeast RAP1 protein
Authors
Issue Date1990
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 1990, v. 18 n. 9, p. 2617-2623 How to Cite?
AbstractThe 827 amino acid yeast RAP1 protein interacts with DNA to regulate gene expression at numerous unrelated loci in the yeast genome. By a combination of amino, carboxy and internal deletions, we have defined an internal 235 amino acid fragment of the yeast RAP1 protein that can bind efficiently to the RAP1 binding site of the PGK Upstream Activation Sequence (UAS). This domain spans residues 361 to 596 of the full length protein and lacks any homology to the DNA binding 'zinc finger' or helix-turn-helix' structural motifs. All the RAP1 binding sites we have tested bind domain 361-596, arguing that RAP1 binds all its chromosomal sites via this domain. The domain could not be further reduced in size suggesting that it represents the minimal functional DNA binding domain. The relevance of potential regions of secondary structure within the minimal binding domain is discussed.
Persistent Identifierhttp://hdl.handle.net/10722/178495
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHenry, YALen_US
dc.contributor.authorChambers, Aen_US
dc.contributor.authorTsang, JSHen_US
dc.contributor.authorKingsman, AJen_US
dc.contributor.authorKingsman, SMen_US
dc.date.accessioned2012-12-19T09:48:01Z-
dc.date.available2012-12-19T09:48:01Z-
dc.date.issued1990en_US
dc.identifier.citationNucleic Acids Research, 1990, v. 18 n. 9, p. 2617-2623en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://hdl.handle.net/10722/178495-
dc.description.abstractThe 827 amino acid yeast RAP1 protein interacts with DNA to regulate gene expression at numerous unrelated loci in the yeast genome. By a combination of amino, carboxy and internal deletions, we have defined an internal 235 amino acid fragment of the yeast RAP1 protein that can bind efficiently to the RAP1 binding site of the PGK Upstream Activation Sequence (UAS). This domain spans residues 361 to 596 of the full length protein and lacks any homology to the DNA binding 'zinc finger' or helix-turn-helix' structural motifs. All the RAP1 binding sites we have tested bind domain 361-596, arguing that RAP1 binds all its chromosomal sites via this domain. The domain could not be further reduced in size suggesting that it represents the minimal functional DNA binding domain. The relevance of potential regions of secondary structure within the minimal binding domain is discussed.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshDna, Fungal - Metabolismen_US
dc.subject.meshDna-Binding Proteins - Metabolismen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshFungal Proteins - Metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshProtein Conformationen_US
dc.subject.meshRegulatory Sequences, Nucleic Aciden_US
dc.subject.meshRestriction Mappingen_US
dc.subject.meshSaccharomyces Cerevisiae - Geneticsen_US
dc.subject.meshTranscription Factorsen_US
dc.titleCharacterisation of the DNA binding domain of the yeast RAP1 proteinen_US
dc.typeArticleen_US
dc.identifier.emailTsang, JSH: jshtsang@hku.hken_US
dc.identifier.authorityTsang, JSH=rp00792en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/nar/18.9.2617-
dc.identifier.pmid2187178-
dc.identifier.scopuseid_2-s2.0-0025363618en_US
dc.identifier.volume18en_US
dc.identifier.issue9en_US
dc.identifier.spage2617en_US
dc.identifier.epage2623en_US
dc.identifier.isiWOS:A1990DD51000004-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHenry, YAL=26642929300en_US
dc.identifier.scopusauthoridChambers, A=35563879400en_US
dc.identifier.scopusauthoridTsang, JSH=7102483508en_US
dc.identifier.scopusauthoridKingsman, AJ=7006672393en_US
dc.identifier.scopusauthoridKingsman, SM=7004417560en_US

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