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Article: The rice phytochrome gene: structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region.

TitleThe rice phytochrome gene: structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region.
Authors
Issue Date1989
PublisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantcell.org
Citation
The Plant Cell, 1989, v. 1 n. 3, p. 351-360 How to Cite?
AbstractWe have isolated and characterized both cDNA and genomic clones encoding the apoprotein of rice phytochrome. The mRNA produced from this gene is expressed at a low level in etiolated leaves. Following a flash of red light, the steady-state mRNA level decreases within 15 minutes, and is barely detectable after 2 hours. This effect is partially reversed by far red light demonstrating autoregulation of phytochrome mRNA levels. Nuclear run-on experiments show that this effect is exerted on transcription of the phytochrome gene. In etiolated plants, phytochrome mRNA is twofold higher in leaves than in roots, whereas the reverse is true in fully green plants where phytochrome mRNA accumulates despite illumination of the leaves. DNA gel blots and screening of libraries indicate the presence of only a single gene, allowing convenient study of the autoregulatory phenomenon for a specific phytochrome gene. Gel retardation analysis using a fragment from the 5' upstream region reveals that GT-1 is present in nuclear extracts of etiolated rice leaves and binds to sites conserved between rice and oat phytochrome genes.
Persistent Identifierhttp://hdl.handle.net/10722/178472
ISSN
2015 Impact Factor: 8.538
2015 SCImago Journal Rankings: 5.706

 

DC FieldValueLanguage
dc.contributor.authorKay, SAen_US
dc.contributor.authorKeith, Ben_US
dc.contributor.authorShinozaki, Ken_US
dc.contributor.authorChye, MLen_US
dc.contributor.authorChua, NHen_US
dc.date.accessioned2012-12-19T09:47:53Z-
dc.date.available2012-12-19T09:47:53Z-
dc.date.issued1989en_US
dc.identifier.citationThe Plant Cell, 1989, v. 1 n. 3, p. 351-360en_US
dc.identifier.issn1040-4651en_US
dc.identifier.urihttp://hdl.handle.net/10722/178472-
dc.description.abstractWe have isolated and characterized both cDNA and genomic clones encoding the apoprotein of rice phytochrome. The mRNA produced from this gene is expressed at a low level in etiolated leaves. Following a flash of red light, the steady-state mRNA level decreases within 15 minutes, and is barely detectable after 2 hours. This effect is partially reversed by far red light demonstrating autoregulation of phytochrome mRNA levels. Nuclear run-on experiments show that this effect is exerted on transcription of the phytochrome gene. In etiolated plants, phytochrome mRNA is twofold higher in leaves than in roots, whereas the reverse is true in fully green plants where phytochrome mRNA accumulates despite illumination of the leaves. DNA gel blots and screening of libraries indicate the presence of only a single gene, allowing convenient study of the autoregulatory phenomenon for a specific phytochrome gene. Gel retardation analysis using a fragment from the 5' upstream region reveals that GT-1 is present in nuclear extracts of etiolated rice leaves and binds to sites conserved between rice and oat phytochrome genes.en_US
dc.languageengen_US
dc.publisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantcell.orgen_US
dc.relation.ispartofThe Plant cellen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna - Metabolismen_US
dc.subject.meshDna-Binding Proteins - Metabolismen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOryza Sativa - Geneticsen_US
dc.subject.meshPhytochrome - Geneticsen_US
dc.subject.meshRestriction Mappingen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleThe rice phytochrome gene: structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region.en_US
dc.typeArticleen_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1105/tpc.1.3.351en_US
dc.identifier.pmid2535506-
dc.identifier.scopuseid_2-s2.0-0024619567en_US
dc.identifier.volume1en_US
dc.identifier.issue3en_US
dc.identifier.spage351en_US
dc.identifier.epage360en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKay, SA=7202568608en_US
dc.identifier.scopusauthoridKeith, B=7005242539en_US
dc.identifier.scopusauthoridShinozaki, K=22939092300en_US
dc.identifier.scopusauthoridChye, ML=7003905460en_US
dc.identifier.scopusauthoridChua, NH=7103077967en_US

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