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Article: Racemization assessment in alkali treated dietary proteins using high-performance liquid chromatography
Title | Racemization assessment in alkali treated dietary proteins using high-performance liquid chromatography |
---|---|
Authors | |
Keywords | oat protein racemization rapeseed protein |
Issue Date | 1989 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/nutres |
Citation | Nutrition Research, 1989, v. 9 n. 9, p. 1053-1065 How to Cite? |
Abstract | Racemization of amino acids was studied in alkali treated β-casein, oat protein (Avena sativa) and rapeseed protein (Brasica napus). Amino acid hydrolyzates containing enantiomeric amino acids, L-Xxx and D-Xxx, were converted into mixtures of N-ethoxycarbonyl-(Eoc-) derivatized diastereomeric dipeptide pairs, Eoc-Phe-L-Xxx, Eoc-Phe-D-Xxx and Eoc-Val-L-Xxx, Eoc-Val-D-Xxx upon the reaction with ethoxycarbonylphenylalanine N-hydroxysuccinimide ester (Eoc-Phe-ONSU) and ethoxycarbonylvaline N-hydroxysuccinimide ester (Eoc-Val-ONSU), respectively. The epimeric products were separated and determined by reversed phase high-performance liquid chromatography and the content of D-isomers was calculated. Racemization of β-casein using this method was in agreement with gas chromatography-mass spectrometry techniques described in the literature. In oat and rapeseed proteins exposed to alkaline conditions for 3 and 24 hours in 0.1 N NaOH at 65°C, the extend of racemization followed the sequence Ser>Asp>Phe>Glu>Val in most of the samples. In preliminar experiments, alkali-treated sunflower seed protein (Helian-thus annuus) samples were examined for the content of D-Phe. The reported HPLC technique represents a simple method for a practical assessment of racemization in dietary proteins. |
Persistent Identifier | http://hdl.handle.net/10722/178469 |
ISSN | 2023 Impact Factor: 3.4 2023 SCImago Journal Rankings: 0.951 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Paquet, A | en_US |
dc.contributor.author | Ma, CY | en_US |
dc.date.accessioned | 2012-12-19T09:47:52Z | - |
dc.date.available | 2012-12-19T09:47:52Z | - |
dc.date.issued | 1989 | en_US |
dc.identifier.citation | Nutrition Research, 1989, v. 9 n. 9, p. 1053-1065 | en_US |
dc.identifier.issn | 0271-5317 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178469 | - |
dc.description.abstract | Racemization of amino acids was studied in alkali treated β-casein, oat protein (Avena sativa) and rapeseed protein (Brasica napus). Amino acid hydrolyzates containing enantiomeric amino acids, L-Xxx and D-Xxx, were converted into mixtures of N-ethoxycarbonyl-(Eoc-) derivatized diastereomeric dipeptide pairs, Eoc-Phe-L-Xxx, Eoc-Phe-D-Xxx and Eoc-Val-L-Xxx, Eoc-Val-D-Xxx upon the reaction with ethoxycarbonylphenylalanine N-hydroxysuccinimide ester (Eoc-Phe-ONSU) and ethoxycarbonylvaline N-hydroxysuccinimide ester (Eoc-Val-ONSU), respectively. The epimeric products were separated and determined by reversed phase high-performance liquid chromatography and the content of D-isomers was calculated. Racemization of β-casein using this method was in agreement with gas chromatography-mass spectrometry techniques described in the literature. In oat and rapeseed proteins exposed to alkaline conditions for 3 and 24 hours in 0.1 N NaOH at 65°C, the extend of racemization followed the sequence Ser>Asp>Phe>Glu>Val in most of the samples. In preliminar experiments, alkali-treated sunflower seed protein (Helian-thus annuus) samples were examined for the content of D-Phe. The reported HPLC technique represents a simple method for a practical assessment of racemization in dietary proteins. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/nutres | en_US |
dc.relation.ispartof | Nutrition Research | en_US |
dc.subject | oat protein | - |
dc.subject | racemization | - |
dc.subject | rapeseed protein | - |
dc.title | Racemization assessment in alkali treated dietary proteins using high-performance liquid chromatography | en_US |
dc.type | Article | en_US |
dc.identifier.email | Ma, CY: macy@hkucc.hku.hk | en_US |
dc.identifier.authority | Ma, CY=rp00759 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.scopus | eid_2-s2.0-0024386971 | en_US |
dc.identifier.volume | 9 | en_US |
dc.identifier.issue | 9 | en_US |
dc.identifier.spage | 1053 | en_US |
dc.identifier.epage | 1065 | en_US |
dc.identifier.isi | WOS:A1989AU92900012 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Paquet, A=7003782027 | en_US |
dc.identifier.scopusauthorid | Ma, CY=7402924944 | en_US |
dc.identifier.issnl | 0271-5317 | - |