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Article: Transcriptional control of the Saccharomyces cerevisiae PGK gene by RAP1

TitleTranscriptional control of the Saccharomyces cerevisiae PGK gene by RAP1
Authors
Issue Date1989
Citation
Molecular And Cellular Biology, 1989, v. 9 n. 12, p. 5516-5524 How to Cite?
AbstractThe promoter of the yeast glycolytic gene encoding phosphoglycerate kinase (PGK) contains an upstream activation sequence between bases -538 and -402 upstream of the initiating ATG. The upstream activation sequence contains multiple functional elements, including an essential region called the activator core (AC) sequence and three copies of the pentamer 5'-CTTCC-3'. The AC sequence shows strong homology to the consensus binding sites for the yeast proteins RAP1 (GRF1) and TUF. We have demonstrated that the yeast protein which interacts with the AC sequence is the DNA-binding protein RAP1. Expression of the PGK gene is found to be regulated according to the carbon source in the growth medium. PGK mRNA levels are high in yeast cells grown in glucose medium but low in yeast cells grown in media containing carbon sources such as pyruvate and acetate. This carbon source regulation of transcription was found to be mediated, in part, via regulation of RAP1 binding to the AC sequence. The promoters of many other yeast glycolytic genes also contain consensus RAP1-binding sites and copies of the CTTCC pentamer. This suggests that RAP1 may be involved in transcriptional control of many other glycolytic genes in addition to the PGK gene.
Persistent Identifierhttp://hdl.handle.net/10722/178468
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChambers, Aen_US
dc.contributor.authorTsang, JSHen_US
dc.contributor.authorStanway, Cen_US
dc.contributor.authorKinsgman, AJen_US
dc.contributor.authorKingsman, SMen_US
dc.date.accessioned2012-12-19T09:47:52Z-
dc.date.available2012-12-19T09:47:52Z-
dc.date.issued1989en_US
dc.identifier.citationMolecular And Cellular Biology, 1989, v. 9 n. 12, p. 5516-5524en_US
dc.identifier.issn0270-7306en_US
dc.identifier.urihttp://hdl.handle.net/10722/178468-
dc.description.abstractThe promoter of the yeast glycolytic gene encoding phosphoglycerate kinase (PGK) contains an upstream activation sequence between bases -538 and -402 upstream of the initiating ATG. The upstream activation sequence contains multiple functional elements, including an essential region called the activator core (AC) sequence and three copies of the pentamer 5'-CTTCC-3'. The AC sequence shows strong homology to the consensus binding sites for the yeast proteins RAP1 (GRF1) and TUF. We have demonstrated that the yeast protein which interacts with the AC sequence is the DNA-binding protein RAP1. Expression of the PGK gene is found to be regulated according to the carbon source in the growth medium. PGK mRNA levels are high in yeast cells grown in glucose medium but low in yeast cells grown in media containing carbon sources such as pyruvate and acetate. This carbon source regulation of transcription was found to be mediated, in part, via regulation of RAP1 binding to the AC sequence. The promoters of many other yeast glycolytic genes also contain consensus RAP1-binding sites and copies of the CTTCC pentamer. This suggests that RAP1 may be involved in transcriptional control of many other glycolytic genes in addition to the PGK gene.en_US
dc.languageengen_US
dc.relation.ispartofMolecular and Cellular Biologyen_US
dc.subject.meshAntibodiesen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshDna-Binding Proteins - Metabolismen_US
dc.subject.meshFungal Proteins - Biosynthesis - Metabolismen_US
dc.subject.meshGene Expression Regulation, Enzymologicen_US
dc.subject.meshGene Expression Regulation, Fungalen_US
dc.subject.meshGenes, Fungalen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPhosphoglycerate Kinase - Geneticsen_US
dc.subject.meshRna, Messenger - Analysis - Geneticsen_US
dc.subject.meshRestriction Mappingen_US
dc.subject.meshSaccharomyces Cerevisiae - Enzymology - Geneticsen_US
dc.subject.meshTranscription Factorsen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleTranscriptional control of the Saccharomyces cerevisiae PGK gene by RAP1en_US
dc.typeArticleen_US
dc.identifier.emailTsang, JSH: jshtsang@hku.hken_US
dc.identifier.authorityTsang, JSH=rp00792en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2685568-
dc.identifier.scopuseid_2-s2.0-0024374498en_US
dc.identifier.volume9en_US
dc.identifier.issue12en_US
dc.identifier.spage5516en_US
dc.identifier.epage5524en_US
dc.identifier.isiWOS:A1989CB55100029-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChambers, A=35563879400en_US
dc.identifier.scopusauthoridTsang, JSH=7102483508en_US
dc.identifier.scopusauthoridStanway, C=6603949543en_US
dc.identifier.scopusauthoridKinsgman, AJ=6504424443en_US
dc.identifier.scopusauthoridKingsman, SM=7004417560en_US

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