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Article: A comparison of bovine growth hormone expression directed by bGH genomic or intronless DNA in transiently transfected eukaryotic cells.

TitleA comparison of bovine growth hormone expression directed by bGH genomic or intronless DNA in transiently transfected eukaryotic cells.
Authors
Pasleau, FLeung, FKopchick, JJ
Keywordsavian retrovirus LTR
DEAE-dextran transfections
expression vector
mouse L cells
Recombinant DNA
Issue Date1987
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene
Citation
Gene, 1987, v. 57 n. 1, p. 47-52 How to Cite?
DOI: http://dx.doi.org/10.1016/0378-1119(87)90175-2
AbstractTwo recombinant DNA plasmids were constructed with identical transcriptional and translational regulatory elements controlling expression of the bovine growth hormone (bGH) gene or the bGH gene lacking introns. Transient expression of these plasmids in culture eukaryotic cells, monitored by assaying secretion of bGH into the culture medium, was employed to examine the relative importance of introns in the expression of this gene. The bGH gene lacking introns is expressed more efficiently than the bGH gene in avian and mammalian cells.
Persistent Identifierhttp://hdl.handle.net/10722/178457
ISSN
0378-1119
2021 Impact Factor: 3.913
2020 SCImago Journal Rankings: 0.916
ISI Accession Number ID
WOS:A1987K756900006

 

DC FieldValueLanguage
dc.contributor.authorPasleau, Fen_US
dc.contributor.authorLeung, Fen_US
dc.contributor.authorKopchick, JJen_US
dc.date.accessioned2012-12-19T09:47:47Z-
dc.date.available2012-12-19T09:47:47Z-
dc.date.issued1987en_US
dc.identifier.citationGene, 1987, v. 57 n. 1, p. 47-52en_US
dc.identifier.issn0378-1119en_US
dc.identifier.urihttp://hdl.handle.net/10722/178457-
dc.description.abstractTwo recombinant DNA plasmids were constructed with identical transcriptional and translational regulatory elements controlling expression of the bovine growth hormone (bGH) gene or the bGH gene lacking introns. Transient expression of these plasmids in culture eukaryotic cells, monitored by assaying secretion of bGH into the culture medium, was employed to examine the relative importance of introns in the expression of this gene. The bGH gene lacking introns is expressed more efficiently than the bGH gene in avian and mammalian cells.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/geneen_US
dc.relation.ispartofGeneen_US
dc.subjectavian retrovirus LTR-
dc.subjectDEAE-dextran transfections-
dc.subjectexpression vector-
dc.subjectmouse L cells-
dc.subjectRecombinant DNA-
dc.subject.meshAnimalsen_US
dc.subject.meshCattleen_US
dc.subject.meshDna Restriction Enzymesen_US
dc.subject.meshGenesen_US
dc.subject.meshGenes, Regulatoren_US
dc.subject.meshGrowth Hormone - Geneticsen_US
dc.subject.meshIntronsen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTransfectionen_US
dc.titleA comparison of bovine growth hormone expression directed by bGH genomic or intronless DNA in transiently transfected eukaryotic cells.en_US
dc.typeArticleen_US
dc.identifier.emailLeung, F: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, F=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0378-1119(87)90175-2-
dc.identifier.pmid2828181-
dc.identifier.scopuseid_2-s2.0-0023199466en_US
dc.identifier.volume57en_US
dc.identifier.issue1en_US
dc.identifier.spage47en_US
dc.identifier.epage52en_US
dc.identifier.isiWOS:A1987K756900006-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridPasleau, F=6602126841en_US
dc.identifier.scopusauthoridLeung, F=7103078633en_US
dc.identifier.scopusauthoridKopchick, JJ=7005438073en_US
dc.identifier.issnl0378-1119-

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