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Article: Homologous and heterologous growth hormones fail to stimulate avian cartilage growth in vitro

TitleHomologous and heterologous growth hormones fail to stimulate avian cartilage growth in vitro
Authors
Issue Date1985
PublisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.org
Citation
Journal Of Clinical Endocrinology And Metabolism, 1985, v. 60 n. 4, p. 747-750 How to Cite?
AbstractRecent reports that GH has a direct effect on growing cartilage have raised questions as to the role of somatomedins (Sm) in cartilage growth. To test the hypothesis that GH directly stimulates cartilage growth, we added homologous and heterologous GHs to organ cultures of embryonic chick pelvic cartilage. Pelvic rudiments from 9-day-old chick embryos were incubated in serum-free medium for 3 days in medium alone or medium containing chicken GH, turkey GH, bovine GH, human GH, and bovine GH produced by recombinant DNA methodology. None of the GH preparations studied stimulated avian cartilage growth in vitro. However, cartilage wet weight increased in response to sera from normal and growth hormone-treated hypophysectomized rats. In addition, 20 ng/ml purified Sm-C caused a 78% increase in cartilage weight above that of cartilage incubated in medium alone. Insulin also caused an increase in cartilage weight, but in concentrations 50,000-100,000 times that of Sm-C. Our studies demonstrate that homologous and heterologous GH have no effect on growing avian cartilage and support the hypothesis that Sm directly mediate cartilage growth.
Persistent Identifierhttp://hdl.handle.net/10722/178432
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.940
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBurch, WMen_US
dc.contributor.authorCorda, Gen_US
dc.contributor.authorKopchick, JJen_US
dc.contributor.authorLeung, FCen_US
dc.date.accessioned2012-12-19T09:47:40Z-
dc.date.available2012-12-19T09:47:40Z-
dc.date.issued1985en_US
dc.identifier.citationJournal Of Clinical Endocrinology And Metabolism, 1985, v. 60 n. 4, p. 747-750en_US
dc.identifier.issn0021-972Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/178432-
dc.description.abstractRecent reports that GH has a direct effect on growing cartilage have raised questions as to the role of somatomedins (Sm) in cartilage growth. To test the hypothesis that GH directly stimulates cartilage growth, we added homologous and heterologous GHs to organ cultures of embryonic chick pelvic cartilage. Pelvic rudiments from 9-day-old chick embryos were incubated in serum-free medium for 3 days in medium alone or medium containing chicken GH, turkey GH, bovine GH, human GH, and bovine GH produced by recombinant DNA methodology. None of the GH preparations studied stimulated avian cartilage growth in vitro. However, cartilage wet weight increased in response to sera from normal and growth hormone-treated hypophysectomized rats. In addition, 20 ng/ml purified Sm-C caused a 78% increase in cartilage weight above that of cartilage incubated in medium alone. Insulin also caused an increase in cartilage weight, but in concentrations 50,000-100,000 times that of Sm-C. Our studies demonstrate that homologous and heterologous GH have no effect on growing avian cartilage and support the hypothesis that Sm directly mediate cartilage growth.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.orgen_US
dc.relation.ispartofJournal of Clinical Endocrinology and Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCartilage - Growth & Developmenten_US
dc.subject.meshCattleen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshChickensen_US
dc.subject.meshGrowth Hormone - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInsulin-Like Growth Factor Ien_US
dc.subject.meshSomatomedins - Pharmacologyen_US
dc.subject.meshSpecies Specificityen_US
dc.subject.meshTurkeysen_US
dc.titleHomologous and heterologous growth hormones fail to stimulate avian cartilage growth in vitroen_US
dc.typeArticleen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/jcem-60-4-747-
dc.identifier.pmid4038715-
dc.identifier.scopuseid_2-s2.0-0021912171en_US
dc.identifier.volume60en_US
dc.identifier.issue4en_US
dc.identifier.spage747en_US
dc.identifier.epage750en_US
dc.identifier.isiWOS:A1985ADU0900021-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBurch, WM=7005878497en_US
dc.identifier.scopusauthoridCorda, G=6603920466en_US
dc.identifier.scopusauthoridKopchick, JJ=7005438073en_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US

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