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Article: Mechanism(s) by which adrenalectomy and corticosterone influence prolactin release in the rat

TitleMechanism(s) by which adrenalectomy and corticosterone influence prolactin release in the rat
Authors
Issue Date1980
PublisherSociety for Endocrinology. The Journal's web site is located at http://joe.endocrinology-journals.org
Citation
Journal Of Endocrinology, 1980, v. 87 n. 1, p. 131-140 How to Cite?
AbstractThe possible direct effect of corticosterone on release of pituitary prolactin was examined in a system using incubation for 8 h. Corticosterone at either 0.1 or 1 μg/ml medium had no significant effect on in-vitro prolactin release but 10 or 100 μg/ml medium produced a significant inhibition of release of prolactin. Release of LH, FSH and thyroid-stimulating hormone were not altered by 0.1, 1 or 10 μg corticosterone/ml, indicating that its action at the concentration of 10 μg/ml was specific on release of prolactin. Corticosterone injected at doses of 1 or 5 mg/kg into hypophysectomized rats with two pituitary grafts underneath the kidney capsule produced a significant fall in serum levels of prolactin when compared with control hypophysectomized rats with two pituitary grafts. Examination with the electron microscope showed that about one third of the lactotrophes from adrenalectomized rats after corticosterone injection exhibited patterns which suggested a decrease in protein synthesis when compared with lactotrophes from adrenalectomized rats given only the vehicle injection. These observations indicated that inhibition of release of prolactin by corticosterone could be exerted directly on the pituitary gland, that the rise of serum levels of prolactin after adrenalectomy might have been due to the removal of direct inhibition by corticosterone. Male rats were adrenalectomized and 2-3 weeks later, concentrations of dopamine and noradrenaline in the medial basal hypothalamus were measured and found not to be different from values in intact rats. Dopamine metabolism also was not altered in the median eminence. The dopaminergic agonist, L-DOPA, inhibited, and the antagonists, pimozide and haloperidol, stimulated release of prolactin in both adrenalectomized and intact rats. Serotonin (5-HT) metabolism in the medial basal hypothalamus and anterior hypothalamus of adrenalectomized rats was not significantly different from values in intact rats, but a higher concentration of 5-HT was observed in the medial basal hypothalamus of adrenalectomized rats when compared with the values in intact rats. A serotonergic agonist, fluoxetine, and an antagonist, cyproheptadine, had no apparent effect on release of prolactin in intact rats, but fluoxetine produced a significant rise, and cyproheptadine, a significant lowering of serum levels of prolactin in adrenalectomized rats. These results suggest that 5-HT, but not dopamine, may be involved in the rise of prolactin after adrenalectomy.
Persistent Identifierhttp://hdl.handle.net/10722/178410
ISSN
2015 Impact Factor: 4.498
2015 SCImago Journal Rankings: 1.910
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, FCen_US
dc.contributor.authorChen, HTen_US
dc.contributor.authorVerkaik, SJen_US
dc.contributor.authorSteger, RWen_US
dc.contributor.authorPeluso, JJen_US
dc.contributor.authorCampbell, GAen_US
dc.contributor.authorMeites, Jen_US
dc.date.accessioned2012-12-19T09:47:34Z-
dc.date.available2012-12-19T09:47:34Z-
dc.date.issued1980en_US
dc.identifier.citationJournal Of Endocrinology, 1980, v. 87 n. 1, p. 131-140en_US
dc.identifier.issn0022-0795en_US
dc.identifier.urihttp://hdl.handle.net/10722/178410-
dc.description.abstractThe possible direct effect of corticosterone on release of pituitary prolactin was examined in a system using incubation for 8 h. Corticosterone at either 0.1 or 1 μg/ml medium had no significant effect on in-vitro prolactin release but 10 or 100 μg/ml medium produced a significant inhibition of release of prolactin. Release of LH, FSH and thyroid-stimulating hormone were not altered by 0.1, 1 or 10 μg corticosterone/ml, indicating that its action at the concentration of 10 μg/ml was specific on release of prolactin. Corticosterone injected at doses of 1 or 5 mg/kg into hypophysectomized rats with two pituitary grafts underneath the kidney capsule produced a significant fall in serum levels of prolactin when compared with control hypophysectomized rats with two pituitary grafts. Examination with the electron microscope showed that about one third of the lactotrophes from adrenalectomized rats after corticosterone injection exhibited patterns which suggested a decrease in protein synthesis when compared with lactotrophes from adrenalectomized rats given only the vehicle injection. These observations indicated that inhibition of release of prolactin by corticosterone could be exerted directly on the pituitary gland, that the rise of serum levels of prolactin after adrenalectomy might have been due to the removal of direct inhibition by corticosterone. Male rats were adrenalectomized and 2-3 weeks later, concentrations of dopamine and noradrenaline in the medial basal hypothalamus were measured and found not to be different from values in intact rats. Dopamine metabolism also was not altered in the median eminence. The dopaminergic agonist, L-DOPA, inhibited, and the antagonists, pimozide and haloperidol, stimulated release of prolactin in both adrenalectomized and intact rats. Serotonin (5-HT) metabolism in the medial basal hypothalamus and anterior hypothalamus of adrenalectomized rats was not significantly different from values in intact rats, but a higher concentration of 5-HT was observed in the medial basal hypothalamus of adrenalectomized rats when compared with the values in intact rats. A serotonergic agonist, fluoxetine, and an antagonist, cyproheptadine, had no apparent effect on release of prolactin in intact rats, but fluoxetine produced a significant rise, and cyproheptadine, a significant lowering of serum levels of prolactin in adrenalectomized rats. These results suggest that 5-HT, but not dopamine, may be involved in the rise of prolactin after adrenalectomy.en_US
dc.languageengen_US
dc.publisherSociety for Endocrinology. The Journal's web site is located at http://joe.endocrinology-journals.orgen_US
dc.relation.ispartofJournal of Endocrinologyen_US
dc.subject.meshAdrenalectomyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCatecholamines - Metabolismen_US
dc.subject.meshCorticosterone - Pharmacologyen_US
dc.subject.meshHypophysectomyen_US
dc.subject.meshHypothalamus - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshPituitary Gland - Drug Effects - Ultrastructureen_US
dc.subject.meshProlactin - Blood - Secretionen_US
dc.subject.meshRatsen_US
dc.subject.meshSerotonin - Metabolismen_US
dc.titleMechanism(s) by which adrenalectomy and corticosterone influence prolactin release in the raten_US
dc.typeArticleen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1677/joe.0.0870131-
dc.identifier.pmid7430912-
dc.identifier.scopuseid_2-s2.0-0018966867en_US
dc.identifier.volume87en_US
dc.identifier.issue1en_US
dc.identifier.spage131en_US
dc.identifier.epage140en_US
dc.identifier.isiWOS:A1980KM62500016-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US
dc.identifier.scopusauthoridChen, HT=7501614739en_US
dc.identifier.scopusauthoridVerkaik, SJ=6504144473en_US
dc.identifier.scopusauthoridSteger, RW=7006481900en_US
dc.identifier.scopusauthoridPeluso, JJ=7006739031en_US
dc.identifier.scopusauthoridCampbell, GA=7401889841en_US
dc.identifier.scopusauthoridMeites, J=7101645729en_US

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