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Article: Relationship between radioreceptor assay and radioimmunoassay estimates of prolactin in rat pituitary tissue, incubation medium, and serum: Effects of dialysis on measurements of the hormone

TitleRelationship between radioreceptor assay and radioimmunoassay estimates of prolactin in rat pituitary tissue, incubation medium, and serum: Effects of dialysis on measurements of the hormone
Authors
Issue Date1980
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 1980, v. 106 n. 1, p. 61-67 How to Cite?
AbstractThe relationships between radioreceptorassay (RRA) and RIA estimates of PRL in rat pituitary tissue, in medium in which rat adenohypophyses were incubated, and in serum were determined. The RRA involved 125I-labeled ovine PRL and hepatic PRL receptors from female rats which bore a PRL-secreting pituitary tumor (MtTW15). A homologous RIA from rat PRL was used for comparison. Highly significant correlations were obtained between both assays with the three types of samples, with r values of 0.90, 0.97, and 0.94, respectively. Regression analyses revealed that the RRA estimates of PRL in pituitary homogenate and incubation medium were slightly but significantly higher than those obtained with the RIA, but the RRA measured about 3.5 times more PRL in serum than did the RIA. The results indicate that the RRA gives estimates of PRL in rat serum that are, relative to RIA measurements, quantitatively similar to those obtained previously by bioassay. However, the RRA is subject to some degree of nonspecific interference from cross-reacting materials because serum from hypophysectomized rats with little or no radioimmunoassay active PRL showed significant activity in the RRA. Dialysis reduced the amount of radioreceptor assayable and radioimmunoassayable active PRL in serum to a greater degree than it did with pituitary homogenate or incubation medium, and it eliminated the nonspecific cross-reactivity from most of the serum samples from hypophysectomized rats. Overall, the results also indicate that, with respect to RRA and RIA detectability, PRL in pituitary tissue and incubation medium differs from the hormone in serum.
Persistent Identifierhttp://hdl.handle.net/10722/178409
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, FCen_US
dc.date.accessioned2012-12-19T09:47:33Z-
dc.date.available2012-12-19T09:47:33Z-
dc.date.issued1980en_US
dc.identifier.citationEndocrinology, 1980, v. 106 n. 1, p. 61-67en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10722/178409-
dc.description.abstractThe relationships between radioreceptorassay (RRA) and RIA estimates of PRL in rat pituitary tissue, in medium in which rat adenohypophyses were incubated, and in serum were determined. The RRA involved 125I-labeled ovine PRL and hepatic PRL receptors from female rats which bore a PRL-secreting pituitary tumor (MtTW15). A homologous RIA from rat PRL was used for comparison. Highly significant correlations were obtained between both assays with the three types of samples, with r values of 0.90, 0.97, and 0.94, respectively. Regression analyses revealed that the RRA estimates of PRL in pituitary homogenate and incubation medium were slightly but significantly higher than those obtained with the RIA, but the RRA measured about 3.5 times more PRL in serum than did the RIA. The results indicate that the RRA gives estimates of PRL in rat serum that are, relative to RIA measurements, quantitatively similar to those obtained previously by bioassay. However, the RRA is subject to some degree of nonspecific interference from cross-reacting materials because serum from hypophysectomized rats with little or no radioimmunoassay active PRL showed significant activity in the RRA. Dialysis reduced the amount of radioreceptor assayable and radioimmunoassayable active PRL in serum to a greater degree than it did with pituitary homogenate or incubation medium, and it eliminated the nonspecific cross-reactivity from most of the serum samples from hypophysectomized rats. Overall, the results also indicate that, with respect to RRA and RIA detectability, PRL in pituitary tissue and incubation medium differs from the hormone in serum.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_US
dc.relation.ispartofEndocrinologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDialysisen_US
dc.subject.meshEvaluation Studies As Topicen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshPituitary Gland, Anterior - Analysisen_US
dc.subject.meshProlactin - Analysis - Blooden_US
dc.subject.meshRadioimmunoassay - Methodsen_US
dc.subject.meshRadioligand Assayen_US
dc.subject.meshRatsen_US
dc.titleRelationship between radioreceptor assay and radioimmunoassay estimates of prolactin in rat pituitary tissue, incubation medium, and serum: Effects of dialysis on measurements of the hormoneen_US
dc.typeArticleen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/endo-106-1-61-
dc.identifier.pmid7349973en_US
dc.identifier.scopuseid_2-s2.0-0018917822en_US
dc.identifier.volume106en_US
dc.identifier.issue1en_US
dc.identifier.spage61en_US
dc.identifier.epage67en_US
dc.identifier.isiWOS:A1980HY97500011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US

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