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Article: Relationship between bioassay and radioimmunoassay estimates of prolactin in rat serum

TitleRelationship between bioassay and radioimmunoassay estimates of prolactin in rat serum
Authors
Issue Date1978
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 1978, v. 103 n. 5, p. 1619-1628 How to Cite?
AbstractRIA and bioassay (BA) estimates of the concentration of PRL ([PRL]) in rat serum were compared using homologous assay systems. A rat mammary gland organ culture BA was developed which allowed objective and quantitative measurement of serum [PRL]. The serum samples were tested at two doses against three doses of the RP-1 rat PRL standard and the responses were quantified by a histometric procedure. The index of precision of the BA ranged from 0.1-0.4 and the secretory response was not affected by physiological concentrations of rat GH, T3, or by gonadal steroids. Serum samples taken from female rats during the estrous cycle, from suckled lactators, from rats bearing a PRL-secreting pituitary tumor, and from females that were treated with perphenazine were assayed. A highly significant correlation (r=0.99) was obtained between the two assay methods with 21 samples. However, the regression coefficient was 4.20 ± 0.55. Thus, although the two assays are highly correlated for measuring serum [PRL], the RIA measures only about 25% of the hormone that is detected by the mammary gland organ culture BA.
Persistent Identifierhttp://hdl.handle.net/10722/178401
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, FCen_US
dc.contributor.authorRussell, SMen_US
dc.contributor.authorNicoll, CSen_US
dc.date.accessioned2012-12-19T09:47:30Z-
dc.date.available2012-12-19T09:47:30Z-
dc.date.issued1978en_US
dc.identifier.citationEndocrinology, 1978, v. 103 n. 5, p. 1619-1628en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10722/178401-
dc.description.abstractRIA and bioassay (BA) estimates of the concentration of PRL ([PRL]) in rat serum were compared using homologous assay systems. A rat mammary gland organ culture BA was developed which allowed objective and quantitative measurement of serum [PRL]. The serum samples were tested at two doses against three doses of the RP-1 rat PRL standard and the responses were quantified by a histometric procedure. The index of precision of the BA ranged from 0.1-0.4 and the secretory response was not affected by physiological concentrations of rat GH, T3, or by gonadal steroids. Serum samples taken from female rats during the estrous cycle, from suckled lactators, from rats bearing a PRL-secreting pituitary tumor, and from females that were treated with perphenazine were assayed. A highly significant correlation (r=0.99) was obtained between the two assay methods with 21 samples. However, the regression coefficient was 4.20 ± 0.55. Thus, although the two assays are highly correlated for measuring serum [PRL], the RIA measures only about 25% of the hormone that is detected by the mammary gland organ culture BA.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_US
dc.relation.ispartofEndocrinologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Assayen_US
dc.subject.meshEstrusen_US
dc.subject.meshFemaleen_US
dc.subject.meshGrowth Hormone - Pharmacologyen_US
dc.subject.meshLactationen_US
dc.subject.meshMammary Glands, Animal - Metabolismen_US
dc.subject.meshOrgan Culture Techniquesen_US
dc.subject.meshPerphenazine - Pharmacologyen_US
dc.subject.meshPregnancyen_US
dc.subject.meshProlactin - Blooden_US
dc.subject.meshRadioimmunoassayen_US
dc.subject.meshRatsen_US
dc.subject.meshRegression Analysisen_US
dc.titleRelationship between bioassay and radioimmunoassay estimates of prolactin in rat serumen_US
dc.typeArticleen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/endo-103-5-1619-
dc.identifier.pmid570911en_US
dc.identifier.scopuseid_2-s2.0-0018116748en_US
dc.identifier.volume103en_US
dc.identifier.issue5en_US
dc.identifier.spage1619en_US
dc.identifier.epage1628en_US
dc.identifier.isiWOS:A1978FV57600014-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US
dc.identifier.scopusauthoridRussell, SM=7401537725en_US
dc.identifier.scopusauthoridNicoll, CS=7006466534en_US

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