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- Publisher Website: 10.1007/BF00422284
- Scopus: eid_2-s2.0-0000560575
- WOS: WOS:A1988Q002900006
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Article: A monobromoacetate dehalogenase from Pseudomonas cepacia MBA4
Title | A monobromoacetate dehalogenase from Pseudomonas cepacia MBA4 |
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Authors | |
Keywords | Dehalogenase Monobromoacetic Acid Purification |
Issue Date | 1988 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00203/index.htm |
Citation | Archives Of Microbiology, 1988, v. 150 n. 5, p. 441-446 How to Cite? |
Abstract | Pseudomonas cepacia MBA4 able to utilize monobromoacetic acid as a sole source of carbon and energy was isolated from soil by enrichment culture. In batch culture the ability to utilize the substrate was conferred by a single halidohydrolase-type dehalogenase which demonstrated a high activity towards the enrichment substrate. The purified enzyme, designated as dehalogenase IVa by activity-stain polyacrylamide gel electrophoresis, had a relative molecular weight of 45,000 and was comprised of two electrophoretically identical subunits with relative molecular weights of 23,000. Dehalogenase IVa demonstrated isomer specificity, being active towards the L-isomer of 2-monochloropropionic acid only. The significance of activity-stain polyacrylamide gel electrophoresis in characterizing dehalogenases and their ubiquitous distribution among bacterial genera are discussed. © 1988 Springer-Verlag. |
Persistent Identifier | http://hdl.handle.net/10722/178363 |
ISSN | 2023 Impact Factor: 2.3 2023 SCImago Journal Rankings: 0.589 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Tsang, JSH | en_US |
dc.contributor.author | Sallis, PJ | en_US |
dc.contributor.author | Bull, AT | en_US |
dc.contributor.author | Hardman, DJ | en_US |
dc.date.accessioned | 2012-12-19T09:47:16Z | - |
dc.date.available | 2012-12-19T09:47:16Z | - |
dc.date.issued | 1988 | en_US |
dc.identifier.citation | Archives Of Microbiology, 1988, v. 150 n. 5, p. 441-446 | en_US |
dc.identifier.issn | 0302-8933 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178363 | - |
dc.description.abstract | Pseudomonas cepacia MBA4 able to utilize monobromoacetic acid as a sole source of carbon and energy was isolated from soil by enrichment culture. In batch culture the ability to utilize the substrate was conferred by a single halidohydrolase-type dehalogenase which demonstrated a high activity towards the enrichment substrate. The purified enzyme, designated as dehalogenase IVa by activity-stain polyacrylamide gel electrophoresis, had a relative molecular weight of 45,000 and was comprised of two electrophoretically identical subunits with relative molecular weights of 23,000. Dehalogenase IVa demonstrated isomer specificity, being active towards the L-isomer of 2-monochloropropionic acid only. The significance of activity-stain polyacrylamide gel electrophoresis in characterizing dehalogenases and their ubiquitous distribution among bacterial genera are discussed. © 1988 Springer-Verlag. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00203/index.htm | en_US |
dc.relation.ispartof | Archives of Microbiology | en_US |
dc.subject | Dehalogenase | en_US |
dc.subject | Monobromoacetic Acid | en_US |
dc.subject | Purification | en_US |
dc.title | A monobromoacetate dehalogenase from Pseudomonas cepacia MBA4 | en_US |
dc.type | Article | en_US |
dc.identifier.email | Tsang, JSH: jshtsang@hku.hk | en_US |
dc.identifier.authority | Tsang, JSH=rp00792 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/BF00422284 | en_US |
dc.identifier.scopus | eid_2-s2.0-0000560575 | en_US |
dc.identifier.volume | 150 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 441 | en_US |
dc.identifier.epage | 446 | en_US |
dc.identifier.isi | WOS:A1988Q002900006 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | Tsang, JSH=7102483508 | en_US |
dc.identifier.scopusauthorid | Sallis, PJ=36920491900 | en_US |
dc.identifier.scopusauthorid | Bull, AT=24538530400 | en_US |
dc.identifier.scopusauthorid | Hardman, DJ=7004649669 | en_US |
dc.identifier.issnl | 0302-8933 | - |