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- Publisher Website: 10.1080/095530099139575
- Scopus: eid_2-s2.0-0032851159
- PMID: 10528918
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Article: Combined FISH with pan-telomeric PNA and whole chromosome-specific DNA probes to detect complete and incomplete chromosomal exchanges in human lymphocytes
Title | Combined FISH with pan-telomeric PNA and whole chromosome-specific DNA probes to detect complete and incomplete chromosomal exchanges in human lymphocytes |
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Authors | |
Issue Date | 1999 |
Publisher | Informa Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09553002.asp |
Citation | International Journal Of Radiation Biology, 1999, v. 75 n. 9, p. 1107-1112 How to Cite? |
Abstract | Purpose: To combine FISH with pan-telomeric peptide nucleic acid (PNA) and whole chromosome-specific DNA probes to detect complete and incomplete chromosome exchanges in human lymphocytes. Materials and methods: Human lymphocytes were irradiated in vitro with 0.9 Gy low dose-rate (0.019 Gy/h) tritium β-rays. Metaphase spreads were treated with RNase, fixed in 1:3 acetic acid:methanol, and then further treated with KCl, proteinase K and fixed in 4% paraformaldehyde. Slides were denatured, hybridized for 1.5 h with an FITC-labelled telomeric PNA probe, and rehybridized overnight with a spectrum-orange whole-chromosome probe specific for chromosomes 1, 2 and 4. Hybridized spreads were washed with 70% formamide/20 x SSC and counterstained with DAPI. Results: All three pairs of labelled chromosomes together with 92 telomeres were readily visible after hybridization. The whole chromosomes 1, 2 and 4 were painted orange, and all telomeres were stained green. Unpainted chromosomes were counterstained blue. In the observed 680 chromosome aberrations induced by tritium β-rays in human lymphocytes after 52 h of culture, no evidence of telomere addition was detected. Incomplete and hidden complete exchanges and terminal deletions were definitively discriminated. Conclusion: The simultaneous detection of telomeres and specific whole chromosomes allows for the first time accurate analysis of complete and incomplete chromosome exchanges involving painted chromosomes in human lymphocytes. |
Persistent Identifier | http://hdl.handle.net/10722/178259 |
ISSN | 2023 Impact Factor: 2.1 2023 SCImago Journal Rankings: 0.545 |
References |
DC Field | Value | Language |
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dc.contributor.author | Deng, W | en_US |
dc.contributor.author | Lucas, JN | en_US |
dc.date.accessioned | 2012-12-19T09:44:48Z | - |
dc.date.available | 2012-12-19T09:44:48Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | International Journal Of Radiation Biology, 1999, v. 75 n. 9, p. 1107-1112 | en_US |
dc.identifier.issn | 0955-3002 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178259 | - |
dc.description.abstract | Purpose: To combine FISH with pan-telomeric peptide nucleic acid (PNA) and whole chromosome-specific DNA probes to detect complete and incomplete chromosome exchanges in human lymphocytes. Materials and methods: Human lymphocytes were irradiated in vitro with 0.9 Gy low dose-rate (0.019 Gy/h) tritium β-rays. Metaphase spreads were treated with RNase, fixed in 1:3 acetic acid:methanol, and then further treated with KCl, proteinase K and fixed in 4% paraformaldehyde. Slides were denatured, hybridized for 1.5 h with an FITC-labelled telomeric PNA probe, and rehybridized overnight with a spectrum-orange whole-chromosome probe specific for chromosomes 1, 2 and 4. Hybridized spreads were washed with 70% formamide/20 x SSC and counterstained with DAPI. Results: All three pairs of labelled chromosomes together with 92 telomeres were readily visible after hybridization. The whole chromosomes 1, 2 and 4 were painted orange, and all telomeres were stained green. Unpainted chromosomes were counterstained blue. In the observed 680 chromosome aberrations induced by tritium β-rays in human lymphocytes after 52 h of culture, no evidence of telomere addition was detected. Incomplete and hidden complete exchanges and terminal deletions were definitively discriminated. Conclusion: The simultaneous detection of telomeres and specific whole chromosomes allows for the first time accurate analysis of complete and incomplete chromosome exchanges involving painted chromosomes in human lymphocytes. | en_US |
dc.language | eng | en_US |
dc.publisher | Informa Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09553002.asp | en_US |
dc.relation.ispartof | International Journal of Radiation Biology | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Chromosome Aberrations | en_US |
dc.subject.mesh | Dna Probes | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | In Situ Hybridization, Fluorescence | en_US |
dc.subject.mesh | Lymphocytes - Ultrastructure | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Peptide Nucleic Acids | en_US |
dc.subject.mesh | Telomere | en_US |
dc.title | Combined FISH with pan-telomeric PNA and whole chromosome-specific DNA probes to detect complete and incomplete chromosomal exchanges in human lymphocytes | en_US |
dc.type | Article | en_US |
dc.identifier.email | Deng, W: wdeng@hkucc.hku.hk | en_US |
dc.identifier.authority | Deng, W=rp01640 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1080/095530099139575 | en_US |
dc.identifier.pmid | 10528918 | - |
dc.identifier.scopus | eid_2-s2.0-0032851159 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0032851159&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 75 | en_US |
dc.identifier.issue | 9 | en_US |
dc.identifier.spage | 1107 | en_US |
dc.identifier.epage | 1112 | en_US |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Deng, W=7202223673 | en_US |
dc.identifier.scopusauthorid | Lucas, JN=7402441937 | en_US |
dc.identifier.issnl | 0955-3002 | - |