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Article: Influence of restraint stress on the expression and the serine/threonine phosphatase activity of calcineurin in the rat brain

TitleInfluence of restraint stress on the expression and the serine/threonine phosphatase activity of calcineurin in the rat brain
Authors
Issue Date2001
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/36341/home
Citation
Synapse, 2001, v. 40 n. 2, p. 130-136 How to Cite?
AbstractTo investigate the role of calcineurin (CaN) in the pathogenesis of stress-related psychiatric disorders, we examined the expression of CaN A mRNA and the serine/threonine phosphatase activity of CaN in the rat brain following various restraint stress paradigms. Northern blot analysis revealed no significant changes in the levels of CaN A mRNA expression, in either the frontal cortex or the hippocampus, in response to a single restraint stress for either 15, 45, or 90 min. In addition, no significant change in the levels of CaN A mRNA was found 3 or 6 h after a single restraint stress for 90 min. In situ hybridization analysis revealed that a single restraint stress for 45 min had no influence on CaN A mRNA expression in the CA1 and CA3 pyramidal cell layers or in the dentate gyrus granule cell layer of the hippocampus. However, serine/threonine phosphatase activity was significantly increased in both regions in response to a single restraint stress for either 45 or 90 min. These results demonstrate that the single restraint stress paradigms used in this study increase the activity of CaN without any changes in the expression of CaN, suggesting that the activation of calmodulin and the increased levels of heat shock proteins in response to the restraint stress may upregulate the activity of CaN in the rat brain. © 2001 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/178218
ISSN
2015 Impact Factor: 2.026
2015 SCImago Journal Rankings: 1.059
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWu, YQen_US
dc.contributor.authorBejjani, BAen_US
dc.contributor.authorTsui, LCen_US
dc.contributor.authorMandel, Aen_US
dc.contributor.authorOsborne, LRen_US
dc.contributor.authorShaffer, LGen_US
dc.date.accessioned2012-12-19T09:43:29Z-
dc.date.available2012-12-19T09:43:29Z-
dc.date.issued2001en_US
dc.identifier.citationSynapse, 2001, v. 40 n. 2, p. 130-136en_US
dc.identifier.issn0887-4476en_US
dc.identifier.urihttp://hdl.handle.net/10722/178218-
dc.description.abstractTo investigate the role of calcineurin (CaN) in the pathogenesis of stress-related psychiatric disorders, we examined the expression of CaN A mRNA and the serine/threonine phosphatase activity of CaN in the rat brain following various restraint stress paradigms. Northern blot analysis revealed no significant changes in the levels of CaN A mRNA expression, in either the frontal cortex or the hippocampus, in response to a single restraint stress for either 15, 45, or 90 min. In addition, no significant change in the levels of CaN A mRNA was found 3 or 6 h after a single restraint stress for 90 min. In situ hybridization analysis revealed that a single restraint stress for 45 min had no influence on CaN A mRNA expression in the CA1 and CA3 pyramidal cell layers or in the dentate gyrus granule cell layer of the hippocampus. However, serine/threonine phosphatase activity was significantly increased in both regions in response to a single restraint stress for either 45 or 90 min. These results demonstrate that the single restraint stress paradigms used in this study increase the activity of CaN without any changes in the expression of CaN, suggesting that the activation of calmodulin and the increased levels of heat shock proteins in response to the restraint stress may upregulate the activity of CaN in the rat brain. © 2001 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/36341/homeen_US
dc.relation.ispartofSynapseen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain - Enzymology - Pathologyen_US
dc.subject.meshCalcineurin - Geneticsen_US
dc.subject.meshFrontal Lobe - Enzymology - Pathologyen_US
dc.subject.meshHippocampus - Enzymology - Pathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshPhosphoprotein Phosphatases - Geneticsen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshRestraint, Physical - Adverse Effectsen_US
dc.subject.meshStress, Physiological - Enzymology - Pathology - Physiopathologyen_US
dc.titleInfluence of restraint stress on the expression and the serine/threonine phosphatase activity of calcineurin in the rat brainen_US
dc.typeArticleen_US
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_US
dc.identifier.authorityTsui, LC=rp00058en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/syn.1034en_US
dc.identifier.pmid11252024-
dc.identifier.scopuseid_2-s2.0-0035087312en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035087312&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume40en_US
dc.identifier.issue2en_US
dc.identifier.spage130en_US
dc.identifier.epage136en_US
dc.identifier.isiWOS:000167552100005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWu, YQ=7406891685en_US
dc.identifier.scopusauthoridBejjani, BA=7003548811en_US
dc.identifier.scopusauthoridTsui, LC=7102754167en_US
dc.identifier.scopusauthoridMandel, A=7005727678en_US
dc.identifier.scopusauthoridOsborne, LR=35369973100en_US
dc.identifier.scopusauthoridShaffer, LG=7103378971en_US

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