File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Apoptosis in the retina following subretinal injection of human fetal retinal pigment epithelial cells

TitleApoptosis in the retina following subretinal injection of human fetal retinal pigment epithelial cells
Authors
Issue Date1996
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 1996, v. 37 n. 3, p. S783 How to Cite?
AbstractPurpose. To determine if apoptotic cell death occurs in the retina following subretinal transplantation of human fetal retinal pigment epithelial cells (HFRPE). Methods. Following a standard vitrectomy, 15 μl of cultured HFRPE suspensions were injected transvitreally into the subretinal space of the eyes of pigmented rabbits. 2, 7, and 30 days after the xenogenic transplantation, the animals were euthanized and their eyes enucleated. A second group of rabbits received subretinal injections of 15 μl of PBS and served as controls. The retinas were analyzed for nicked-nuclear DNA in situ by TdT-mediated biotin-dUTP nick-end-labeling (TUNEL) and visualized by fluorescent microscopy. Results. TUNEL-positive photoreceptor cells were observed in both HFRPE-transplanted and control retinas. 2 days after the transplantation, intense and extensive labeling was noted in the outer nuclear layer. The labeled photoreceptor cells were distributed predominantly along the areas of injection. Scattered TUNEL-positive photoreceptor cells were seen in the 7 day group and only occasional labeling was noted in the 30-day group. In addition, the transplanted retinas exhibited more labeling than those of the control group. Conclusions. This study demonstrated that apoptotic photoreceptor cell death occurred following HFRPE transplantation. The retinal detachment created by the subretinal injections may account partly for this observation in both the transplant and control groups. In addition, the more extensive labeling observed in the transplant group may be secondary to an additional insult that resulted from the introduction of foreign cells to the subretinal space.
Persistent Identifierhttp://hdl.handle.net/10722/176456
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008

 

DC FieldValueLanguage
dc.contributor.authorLai, WWen_US
dc.contributor.authorGabrielian, Ken_US
dc.contributor.authorOganesian, Aen_US
dc.contributor.authorPatel, SCen_US
dc.contributor.authorErnest, JTen_US
dc.date.accessioned2012-11-26T09:11:23Z-
dc.date.available2012-11-26T09:11:23Z-
dc.date.issued1996en_US
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 1996, v. 37 n. 3, p. S783en_US
dc.identifier.issn0146-0404en_US
dc.identifier.urihttp://hdl.handle.net/10722/176456-
dc.description.abstractPurpose. To determine if apoptotic cell death occurs in the retina following subretinal transplantation of human fetal retinal pigment epithelial cells (HFRPE). Methods. Following a standard vitrectomy, 15 μl of cultured HFRPE suspensions were injected transvitreally into the subretinal space of the eyes of pigmented rabbits. 2, 7, and 30 days after the xenogenic transplantation, the animals were euthanized and their eyes enucleated. A second group of rabbits received subretinal injections of 15 μl of PBS and served as controls. The retinas were analyzed for nicked-nuclear DNA in situ by TdT-mediated biotin-dUTP nick-end-labeling (TUNEL) and visualized by fluorescent microscopy. Results. TUNEL-positive photoreceptor cells were observed in both HFRPE-transplanted and control retinas. 2 days after the transplantation, intense and extensive labeling was noted in the outer nuclear layer. The labeled photoreceptor cells were distributed predominantly along the areas of injection. Scattered TUNEL-positive photoreceptor cells were seen in the 7 day group and only occasional labeling was noted in the 30-day group. In addition, the transplanted retinas exhibited more labeling than those of the control group. Conclusions. This study demonstrated that apoptotic photoreceptor cell death occurred following HFRPE transplantation. The retinal detachment created by the subretinal injections may account partly for this observation in both the transplant and control groups. In addition, the more extensive labeling observed in the transplant group may be secondary to an additional insult that resulted from the introduction of foreign cells to the subretinal space.en_US
dc.languageengen_US
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_US
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_US
dc.titleApoptosis in the retina following subretinal injection of human fetal retinal pigment epithelial cellsen_US
dc.typeArticleen_US
dc.identifier.emailLai, WW: wicolai@hku.hken_US
dc.identifier.authorityLai, WW=rp00531en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-33750149705en_US
dc.identifier.volume37en_US
dc.identifier.issue3en_US
dc.identifier.spageS783en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLai, WW=7402231098en_US
dc.identifier.scopusauthoridGabrielian, K=6602320216en_US
dc.identifier.scopusauthoridOganesian, A=14719641400en_US
dc.identifier.scopusauthoridPatel, SC=7403903292en_US
dc.identifier.scopusauthoridErnest, JT=7006438134en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats