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Article: Determination of ofloxacin and moxifloxacin and their penetration in human aqueous and vitreous humor by using high-performance liquid chromatography fluorescence detection

TitleDetermination of ofloxacin and moxifloxacin and their penetration in human aqueous and vitreous humor by using high-performance liquid chromatography fluorescence detection
Authors
KeywordsAqueous Humor
Fluorescence Hplc
Moxifloxacin
Ofloxacin
Vitreous Humor
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabio
Citation
Analytical Biochemistry, 2006, v. 353 n. 1, p. 30-36 How to Cite?
AbstractInformation on comparing the penetration of ofloxacin and moxifloxacin in the human eye is unavailable, although these two antibiotics are commonly used in ophthalmic surgery. There is a need for a rapid, reliable, and sensitive methodology for their determination in ocular fluids. We developed a robust HPLC procedure with fluorescence detection for simultaneous analysis of ofloxacin and moxifloxacin in human and rabbit aqueous and vitreous samples. The linearity of the method ranged from 10 ng/ml to 100 μg/ml with r 2 > 0.996. Most inter- and intrabatch imprecision was about 5% (range 1.6-7.6%), recoveries between 95 and 104%, and accuracies between 93 and 104% at 0.1 and 1 μg/ml. The detection limits of both compounds were 10 ng/ml (0.028 nmol/ml for ofloxacin and 0.023 nmol/ml for moxifloxacin). No sample treatment was necessary for aqueous humor and only acetonitrile precipitation was required for vitreous humor. The chromatographic time was short, 22 min. We applied this method to study penetrations of ofloxacin and moxifloxacin in aqueous and vitreous humors of human and rabbits. There was no significant difference of penetration between the two antibiotics into aqueous and vitreous but ofloxacin was found at significantly higher concentrations in aqueous than in vitreous. We also detected contralateral transfer of the antibiotics in rabbit eyes. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/176450
ISSN
2015 Impact Factor: 2.243
2015 SCImago Journal Rankings: 0.725
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, KPen_US
dc.contributor.authorChu, KOen_US
dc.contributor.authorLai, WWKen_US
dc.contributor.authorChoy, KWen_US
dc.contributor.authorWang, CCen_US
dc.contributor.authorLam, DSCen_US
dc.contributor.authorPang, CPen_US
dc.date.accessioned2012-11-26T09:11:21Z-
dc.date.available2012-11-26T09:11:21Z-
dc.date.issued2006en_US
dc.identifier.citationAnalytical Biochemistry, 2006, v. 353 n. 1, p. 30-36en_US
dc.identifier.issn0003-2697en_US
dc.identifier.urihttp://hdl.handle.net/10722/176450-
dc.description.abstractInformation on comparing the penetration of ofloxacin and moxifloxacin in the human eye is unavailable, although these two antibiotics are commonly used in ophthalmic surgery. There is a need for a rapid, reliable, and sensitive methodology for their determination in ocular fluids. We developed a robust HPLC procedure with fluorescence detection for simultaneous analysis of ofloxacin and moxifloxacin in human and rabbit aqueous and vitreous samples. The linearity of the method ranged from 10 ng/ml to 100 μg/ml with r 2 > 0.996. Most inter- and intrabatch imprecision was about 5% (range 1.6-7.6%), recoveries between 95 and 104%, and accuracies between 93 and 104% at 0.1 and 1 μg/ml. The detection limits of both compounds were 10 ng/ml (0.028 nmol/ml for ofloxacin and 0.023 nmol/ml for moxifloxacin). No sample treatment was necessary for aqueous humor and only acetonitrile precipitation was required for vitreous humor. The chromatographic time was short, 22 min. We applied this method to study penetrations of ofloxacin and moxifloxacin in aqueous and vitreous humors of human and rabbits. There was no significant difference of penetration between the two antibiotics into aqueous and vitreous but ofloxacin was found at significantly higher concentrations in aqueous than in vitreous. We also detected contralateral transfer of the antibiotics in rabbit eyes. © 2006 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yabioen_US
dc.relation.ispartofAnalytical Biochemistryen_US
dc.subjectAqueous Humoren_US
dc.subjectFluorescence Hplcen_US
dc.subjectMoxifloxacinen_US
dc.subjectOfloxacinen_US
dc.subjectVitreous Humoren_US
dc.titleDetermination of ofloxacin and moxifloxacin and their penetration in human aqueous and vitreous humor by using high-performance liquid chromatography fluorescence detectionen_US
dc.typeArticleen_US
dc.identifier.emailLai, WWK: wicolai@hku.hken_US
dc.identifier.authorityLai, WWK=rp00531en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/01.rhu.0000200423.12270.18en_US
dc.identifier.pmid16484877-
dc.identifier.scopuseid_2-s2.0-33746565491en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33746565491&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume353en_US
dc.identifier.issue1en_US
dc.identifier.spage30en_US
dc.identifier.epage36en_US
dc.identifier.isiWOS:000235787400006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChan, KP=35336016800en_US
dc.identifier.scopusauthoridChu, KO=7402453672en_US
dc.identifier.scopusauthoridLai, WWK=7402231098en_US
dc.identifier.scopusauthoridChoy, KW=7005477052en_US
dc.identifier.scopusauthoridWang, CC=8859335700en_US
dc.identifier.scopusauthoridLam, DSC=35500200200en_US
dc.identifier.scopusauthoridPang, CP=7201425127en_US

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