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Article: Trafficking of cell-surface β-amyloid precursor protein: Evidence that a sorting intermediate participates in synaptic vesicle recycling

TitleTrafficking of cell-surface β-amyloid precursor protein: Evidence that a sorting intermediate participates in synaptic vesicle recycling
Authors
Issue Date1997
PublisherSociety for Neuroscience. The Journal's web site is located at http://www.jneurosci.org
Citation
Journal Of Neuroscience, 1997, v. 17 n. 1, p. 140-151 How to Cite?
AbstractWe recently demonstrated that the Alzheimer's β-amyloid precursor protein (APP) is internalized from the axonal cell surface. In this study, we use biochemical and cell biological methods to characterize endocytotic compartments that participate in trafficking of APP in central neurons. APP is present in presynaptic clathrin-coated vesicles purified from bovine brain, together with the recycling synaptic vesicle integral membrane proteins synaptophysin, synaptotagmin, and SV2. In contrast, APP is largely excluded from synaptic vesicles purified from rat brain. In primary cerebellar macroneurons, cell-surface APP is internalized with recycling synaptic vesicle integral membrane proteins but is subsequently sorted away from synaptic vesicles and transported retrogradely to the neuronal soma. Internalized APP partially co-localizes with rab5a-containing compartments in axons and with V-ATPase-containing compartments in both axons and neuronal soma. These results provide direct biochemical evidence that an obligate sorting compartment participates in the regeneration of synaptic vesicles during exo/endocytotic recycling at nerve terminals but do not preclude concurrent 'kiss-and-run' recycling. Moreover, APP is now, to our knowledge, the first demonstrated example of an axonal cell-surface protein that is internalized with recycling synaptic vesicle membrane proteins but is subsequently sorted away from synaptic vesicles.
Persistent Identifierhttp://hdl.handle.net/10722/176349
ISSN
2015 Impact Factor: 5.924
2015 SCImago Journal Rankings: 5.105
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMarquezSterling, NRen_US
dc.contributor.authorLo, ACYen_US
dc.contributor.authorSisodia, SSen_US
dc.contributor.authorKoo, EHen_US
dc.date.accessioned2012-11-26T09:10:45Z-
dc.date.available2012-11-26T09:10:45Z-
dc.date.issued1997en_US
dc.identifier.citationJournal Of Neuroscience, 1997, v. 17 n. 1, p. 140-151en_US
dc.identifier.issn0270-6474en_US
dc.identifier.urihttp://hdl.handle.net/10722/176349-
dc.description.abstractWe recently demonstrated that the Alzheimer's β-amyloid precursor protein (APP) is internalized from the axonal cell surface. In this study, we use biochemical and cell biological methods to characterize endocytotic compartments that participate in trafficking of APP in central neurons. APP is present in presynaptic clathrin-coated vesicles purified from bovine brain, together with the recycling synaptic vesicle integral membrane proteins synaptophysin, synaptotagmin, and SV2. In contrast, APP is largely excluded from synaptic vesicles purified from rat brain. In primary cerebellar macroneurons, cell-surface APP is internalized with recycling synaptic vesicle integral membrane proteins but is subsequently sorted away from synaptic vesicles and transported retrogradely to the neuronal soma. Internalized APP partially co-localizes with rab5a-containing compartments in axons and with V-ATPase-containing compartments in both axons and neuronal soma. These results provide direct biochemical evidence that an obligate sorting compartment participates in the regeneration of synaptic vesicles during exo/endocytotic recycling at nerve terminals but do not preclude concurrent 'kiss-and-run' recycling. Moreover, APP is now, to our knowledge, the first demonstrated example of an axonal cell-surface protein that is internalized with recycling synaptic vesicle membrane proteins but is subsequently sorted away from synaptic vesicles.en_US
dc.languageengen_US
dc.publisherSociety for Neuroscience. The Journal's web site is located at http://www.jneurosci.orgen_US
dc.relation.ispartofJournal of Neuroscienceen_US
dc.subject.meshAmyloid Beta-Protein Precursor - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCattleen_US
dc.subject.meshCell Membraneen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCerebellum - Metabolismen_US
dc.subject.meshCerebral Cortex - Cytology - Metabolismen_US
dc.subject.meshClathrin - Metabolismen_US
dc.subject.meshCoated Vesicles - Metabolismen_US
dc.subject.meshEndocytosisen_US
dc.subject.meshMembrane Proteins - Metabolismen_US
dc.subject.meshNeurons - Metabolismen_US
dc.subject.meshPresynaptic Terminals - Metabolismen_US
dc.subject.meshRats - Embryologyen_US
dc.subject.meshSynaptic Vesicles - Physiologyen_US
dc.titleTrafficking of cell-surface β-amyloid precursor protein: Evidence that a sorting intermediate participates in synaptic vesicle recyclingen_US
dc.typeArticleen_US
dc.identifier.emailLo, ACY: amylo@hkucc.hku.hken_US
dc.identifier.authorityLo, ACY=rp00425en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid8987743-
dc.identifier.scopuseid_2-s2.0-0031031006en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031031006&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume17en_US
dc.identifier.issue1en_US
dc.identifier.spage140en_US
dc.identifier.epage151en_US
dc.identifier.isiWOS:A1997WJ67700013-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMarquezSterling, NR=6507568012en_US
dc.identifier.scopusauthoridLo, ACY=7102780640en_US
dc.identifier.scopusauthoridSisodia, SS=7102763509en_US
dc.identifier.scopusauthoridKoo, EH=7005360256en_US

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