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Article: Apoptotic photoreceptor cell death after traumatic retinal detachment in humans

TitleApoptotic photoreceptor cell death after traumatic retinal detachment in humans
Authors
Issue Date1995
PublisherAmerican Medical Association. The Journal's web site is located at http://www.archopthalmol.com
Citation
Archives Of Ophthalmology, 1995, v. 113 n. 7, p. 880-886 How to Cite?
AbstractObjective: To determine the mechanism of photoreceptor cell death after traumatic retinal detachment in humans. Design: Clinical records from 1975 to 1993 of 75 patients, whose eyes were enucleated after traumatic retinal detachment, were reviewed for age, sex, previous ocular or systemic medical history, interval from initial trauma to enucleation, visual acuity, and types of trauma. The patients were divided into five groups of 15 cases each, based on the interval from initial trauma to enucleation. The retinal tissue was examined for two markers of apoptosis: (1) nicked nuclear DNA in situ by the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick end labeling (TUNEL) technique and (2) apoptotic bodies by light and electron microscopy. Result: Of the 75 cases of ruptured globe and traumatic retinal detachment that were evaluated, 19 eyes (25.3%) showed TUNEL-positive labeling of photoreceptor cells. Nicked nuclear DNA was detected in photoreceptor cells of detached retinas as early as 8 hours after trauma. The detached retinas in seven of 15 eyes enucleated within 2 days after ocular trauma showed TUNEL-positive photoreceptor nuclei. The number of cases showing TUNEL-positive photoreceptor nuclei decreased as the interval between initial trauma and enucleation increased. The TUNEL-positive photoreceptor cells could still be seen in the detached retinas of two eyes enucleated 22 days after trauma. Light microscopy disclosed condensation and fragmentation of photoreceptor nuclei in the detached retinas. Electron microscopy showed structures resembling apoptotic bodies phagocytosed by neighboring cells in the TUNEL-positive retinas. Conclusions: Apoptosis is an important mechanism of photoreceptor cell degeneration in the early stage after traumatic retinal detachment in humans.
Persistent Identifierhttp://hdl.handle.net/10722/176340
ISSN
2014 Impact Factor: 4.399
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChang, CJen_US
dc.contributor.authorLai, WWen_US
dc.contributor.authorEdward, DPen_US
dc.contributor.authorTso, MOMen_US
dc.date.accessioned2012-11-26T09:10:40Z-
dc.date.available2012-11-26T09:10:40Z-
dc.date.issued1995en_US
dc.identifier.citationArchives Of Ophthalmology, 1995, v. 113 n. 7, p. 880-886en_US
dc.identifier.issn0003-9950en_US
dc.identifier.urihttp://hdl.handle.net/10722/176340-
dc.description.abstractObjective: To determine the mechanism of photoreceptor cell death after traumatic retinal detachment in humans. Design: Clinical records from 1975 to 1993 of 75 patients, whose eyes were enucleated after traumatic retinal detachment, were reviewed for age, sex, previous ocular or systemic medical history, interval from initial trauma to enucleation, visual acuity, and types of trauma. The patients were divided into five groups of 15 cases each, based on the interval from initial trauma to enucleation. The retinal tissue was examined for two markers of apoptosis: (1) nicked nuclear DNA in situ by the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick end labeling (TUNEL) technique and (2) apoptotic bodies by light and electron microscopy. Result: Of the 75 cases of ruptured globe and traumatic retinal detachment that were evaluated, 19 eyes (25.3%) showed TUNEL-positive labeling of photoreceptor cells. Nicked nuclear DNA was detected in photoreceptor cells of detached retinas as early as 8 hours after trauma. The detached retinas in seven of 15 eyes enucleated within 2 days after ocular trauma showed TUNEL-positive photoreceptor nuclei. The number of cases showing TUNEL-positive photoreceptor nuclei decreased as the interval between initial trauma and enucleation increased. The TUNEL-positive photoreceptor cells could still be seen in the detached retinas of two eyes enucleated 22 days after trauma. Light microscopy disclosed condensation and fragmentation of photoreceptor nuclei in the detached retinas. Electron microscopy showed structures resembling apoptotic bodies phagocytosed by neighboring cells in the TUNEL-positive retinas. Conclusions: Apoptosis is an important mechanism of photoreceptor cell degeneration in the early stage after traumatic retinal detachment in humans.en_US
dc.languageengen_US
dc.publisherAmerican Medical Association. The Journal's web site is located at http://www.archopthalmol.comen_US
dc.relation.ispartofArchives of Ophthalmologyen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshApoptosisen_US
dc.subject.meshCell Deathen_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshDna - Analysisen_US
dc.subject.meshDna Damageen_US
dc.subject.meshDna Nucleotidylexotransferaseen_US
dc.subject.meshEye Enucleationen_US
dc.subject.meshEye Injuries, Penetrating - Complications - Surgeryen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshOrbit - Injuriesen_US
dc.subject.meshPhotoreceptor Cells - Chemistry - Pathologyen_US
dc.subject.meshRetina - Injuries - Pathology - Surgeryen_US
dc.subject.meshRetinal Detachment - Etiology - Pathology - Surgeryen_US
dc.subject.meshRetrospective Studiesen_US
dc.titleApoptotic photoreceptor cell death after traumatic retinal detachment in humansen_US
dc.typeArticleen_US
dc.identifier.emailLai, WW: wicolai@hku.hken_US
dc.identifier.authorityLai, WW=rp00531en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7605279-
dc.identifier.scopuseid_2-s2.0-0028791777en_US
dc.identifier.volume113en_US
dc.identifier.issue7en_US
dc.identifier.spage880en_US
dc.identifier.epage886en_US
dc.identifier.isiWOS:A1995RH81600022-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChang, CJ=8721402600en_US
dc.identifier.scopusauthoridLai, WW=7402231098en_US
dc.identifier.scopusauthoridEdward, DP=7005234763en_US
dc.identifier.scopusauthoridTso, MOM=7102052117en_US

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