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Article: Cloning the human lysozyme cDNA: Inverted Alu repeat in the mRNA and in situ hybridization for macrophages and Paneth cells

TitleCloning the human lysozyme cDNA: Inverted Alu repeat in the mRNA and in situ hybridization for macrophages and Paneth cells
Authors
Issue Date1988
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 1988, v. 85 n. 17, p. 6227-6231 How to Cite?
AbstractLysozyme is a major secretory product of human and rodent macrophages and a useful marker for myelomonocytic cells. Based on the known human lysozyme amino acid sequence, oligonucleotides were synthesized and used as probes to screen a phorbol 12-myristate 13-acetate-treated U937 cDNA library. A full-length human lysozyme cDNA clone, pHL-2, was obtained and characterized. Sequence analysis shows that human lysozyme, like chicken lysozyme, has an 18-amino-acid-long signal peptide, but unlike the chicken lysozyme cDNA, the human lysozyme cDNA has a >1-kilobase-long 3' nontranslated sequence. Interestingly, within this 3' region, an inverted repeat of the Alu family of repetitive sequences was discovered. In RNA blot analyses, DNA probes prepared from pHL-2 can be used to detect lysozyme mRNA not only from human but also from mouse and rat. Moreover, by in situ hybridization, complementary RNA transcripts have been used as probes to detect lysozyme mRNA in mouse macrophages and Paneth cells. This human lysozyme cDNA clone is therefore likely to be a useful molecular probe for studying macrophage distribution and gene expression.
Persistent Identifierhttp://hdl.handle.net/10722/175998
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChung, LPen_US
dc.contributor.authorKeshav, Sen_US
dc.contributor.authorGordon, Sen_US
dc.date.accessioned2012-11-26T09:04:02Z-
dc.date.available2012-11-26T09:04:02Z-
dc.date.issued1988en_US
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 1988, v. 85 n. 17, p. 6227-6231en_US
dc.identifier.issn0027-8424en_US
dc.identifier.urihttp://hdl.handle.net/10722/175998-
dc.description.abstractLysozyme is a major secretory product of human and rodent macrophages and a useful marker for myelomonocytic cells. Based on the known human lysozyme amino acid sequence, oligonucleotides were synthesized and used as probes to screen a phorbol 12-myristate 13-acetate-treated U937 cDNA library. A full-length human lysozyme cDNA clone, pHL-2, was obtained and characterized. Sequence analysis shows that human lysozyme, like chicken lysozyme, has an 18-amino-acid-long signal peptide, but unlike the chicken lysozyme cDNA, the human lysozyme cDNA has a >1-kilobase-long 3' nontranslated sequence. Interestingly, within this 3' region, an inverted repeat of the Alu family of repetitive sequences was discovered. In RNA blot analyses, DNA probes prepared from pHL-2 can be used to detect lysozyme mRNA not only from human but also from mouse and rat. Moreover, by in situ hybridization, complementary RNA transcripts have been used as probes to detect lysozyme mRNA in mouse macrophages and Paneth cells. This human lysozyme cDNA clone is therefore likely to be a useful molecular probe for studying macrophage distribution and gene expression.en_US
dc.languageengen_US
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna - Geneticsen_US
dc.subject.meshEpithelium - Enzymologyen_US
dc.subject.meshGenesen_US
dc.subject.meshHumansen_US
dc.subject.meshMacrophages - Enzymologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMuramidase - Geneticsen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshRna, Messenger - Geneticsen_US
dc.subject.meshRepetitive Sequences, Nucleic Aciden_US
dc.titleCloning the human lysozyme cDNA: Inverted Alu repeat in the mRNA and in situ hybridization for macrophages and Paneth cellsen_US
dc.typeArticleen_US
dc.identifier.emailChung, LP: lpchung@hkucc.hku.hken_US
dc.identifier.authorityChung, LP=rp00249en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1073/pnas.85.17.6227-
dc.identifier.pmid3413092-
dc.identifier.scopuseid_2-s2.0-0023814379en_US
dc.identifier.volume85en_US
dc.identifier.issue17en_US
dc.identifier.spage6227en_US
dc.identifier.epage6231en_US
dc.identifier.isiWOS:A1988Q047600001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChung, LP=24315879100en_US
dc.identifier.scopusauthoridKeshav, S=7003367734en_US
dc.identifier.scopusauthoridGordon, S=35391350600en_US

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