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Article: The role of microsatellite instability in cervical intraepithelial neoplasia and squamous cell carcinoma of the cervix

TitleThe role of microsatellite instability in cervical intraepithelial neoplasia and squamous cell carcinoma of the cervix
Authors
Issue Date2003
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
Citation
Gynecologic Oncology, 2003, v. 89 n. 3, p. 434-439 How to Cite?
AbstractObjectives. This study was conducted to define the role of microsatellite instability (MSI) in cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC) of the cervix. We also tested the validity of using markers recommended for MSI study in colonic carcinoma by the National Cancer Institute (NCI) for cervical neoplasm. Methods. Twenty normal cervical, 24 low-grade CIN (CIN-L), 59 high-grade CIN (CIN-H), and 93 SCC tissues were examined for MSI after microdissection. A polymerase chain reaction based MSI detection was performed using five markers recommended by the NCI for colonic cancer (panel one) as well as five other markers (panel two) found to be informative in earlier studies. High-frequency MSI (MSI-H) was defined as instability in ≥2 of 5 loci if one panel was used and ≥30% of loci when more than five loci were used. Low-frequency MSI (MSI-L) was diagnosed if instability was noted but did not meet the criteria of MSI-H. Findings were correlated with clinicopathologic information. Results. The combined use of panel one and two markers showed no MSI in normal cervical or CIN-L tissue, MSI-L in 1 CIN-H (1.7%), MSI-L in 16 (17.2%), and MSI-H in 11 (11.8%) SCC, respectively. The NCI-recommended panel alone detected 19 of 27 MSI-positive SCC. MSI-positive was not related to patient age, disease stage, and tumor grade. The overall survival of MSI-positive patients was significantly worse than that of microsatellite stable patients (P = 0.02). An increasing trend of MSI-H rate with higher disease stages was noted (P = 0.035) but MSI-H was not associated with poor prognosis. Conclusions. The NCI recommended panel of markers might not be useful in MSI study for SCC and using more than five markers improves the MSI detection. MSI is rare in cervical dysplasia but is present in a subset of SCC. The association between MSI-positivity and prognosis awaits future confirmation. © 2003 Elsevier Science (USA). All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/175888
ISSN
2015 Impact Factor: 4.198
2015 SCImago Journal Rankings: 2.284
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, YFen_US
dc.contributor.authorCheung, THen_US
dc.contributor.authorPoon, KYen_US
dc.contributor.authorWang, VWen_US
dc.contributor.authorLi, JCBen_US
dc.contributor.authorLo, KWKen_US
dc.contributor.authorYim, SFen_US
dc.contributor.authorYu, MYen_US
dc.contributor.authorLahr, Gen_US
dc.contributor.authorChung, TKHen_US
dc.date.accessioned2012-11-26T09:02:11Z-
dc.date.available2012-11-26T09:02:11Z-
dc.date.issued2003en_US
dc.identifier.citationGynecologic Oncology, 2003, v. 89 n. 3, p. 434-439en_US
dc.identifier.issn0090-8258en_US
dc.identifier.urihttp://hdl.handle.net/10722/175888-
dc.description.abstractObjectives. This study was conducted to define the role of microsatellite instability (MSI) in cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC) of the cervix. We also tested the validity of using markers recommended for MSI study in colonic carcinoma by the National Cancer Institute (NCI) for cervical neoplasm. Methods. Twenty normal cervical, 24 low-grade CIN (CIN-L), 59 high-grade CIN (CIN-H), and 93 SCC tissues were examined for MSI after microdissection. A polymerase chain reaction based MSI detection was performed using five markers recommended by the NCI for colonic cancer (panel one) as well as five other markers (panel two) found to be informative in earlier studies. High-frequency MSI (MSI-H) was defined as instability in ≥2 of 5 loci if one panel was used and ≥30% of loci when more than five loci were used. Low-frequency MSI (MSI-L) was diagnosed if instability was noted but did not meet the criteria of MSI-H. Findings were correlated with clinicopathologic information. Results. The combined use of panel one and two markers showed no MSI in normal cervical or CIN-L tissue, MSI-L in 1 CIN-H (1.7%), MSI-L in 16 (17.2%), and MSI-H in 11 (11.8%) SCC, respectively. The NCI-recommended panel alone detected 19 of 27 MSI-positive SCC. MSI-positive was not related to patient age, disease stage, and tumor grade. The overall survival of MSI-positive patients was significantly worse than that of microsatellite stable patients (P = 0.02). An increasing trend of MSI-H rate with higher disease stages was noted (P = 0.035) but MSI-H was not associated with poor prognosis. Conclusions. The NCI recommended panel of markers might not be useful in MSI study for SCC and using more than five markers improves the MSI detection. MSI is rare in cervical dysplasia but is present in a subset of SCC. The association between MSI-positivity and prognosis awaits future confirmation. © 2003 Elsevier Science (USA). All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygynoen_US
dc.relation.ispartofGynecologic Oncologyen_US
dc.subject.meshAdulten_US
dc.subject.meshCarcinoma, Squamous Cell - Genetics - Pathologyen_US
dc.subject.meshCervical Intraepithelial Neoplasia - Genetics - Pathologyen_US
dc.subject.meshDna, Neoplasm - Blood - Geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMicrosatellite Repeats - Geneticsen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNeoplasm Stagingen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshUterine Cervical Neoplasms - Genetics - Pathologyen_US
dc.titleThe role of microsatellite instability in cervical intraepithelial neoplasia and squamous cell carcinoma of the cervixen_US
dc.typeArticleen_US
dc.identifier.emailLi, JCB: jamesli@hku.hken_US
dc.identifier.authorityLi, JCB=rp00496en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0090-8258(03)00134-3en_US
dc.identifier.pmid12798708-
dc.identifier.scopuseid_2-s2.0-0037836075en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037836075&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume89en_US
dc.identifier.issue3en_US
dc.identifier.spage434en_US
dc.identifier.epage439en_US
dc.identifier.isiWOS:000183682300016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, YF=7403041448en_US
dc.identifier.scopusauthoridCheung, TH=7103334169en_US
dc.identifier.scopusauthoridPoon, KY=7006268771en_US
dc.identifier.scopusauthoridWang, VW=7007164250en_US
dc.identifier.scopusauthoridLi, JCB=23103447500en_US
dc.identifier.scopusauthoridLo, KWK=24302880900en_US
dc.identifier.scopusauthoridYim, SF=7101624278en_US
dc.identifier.scopusauthoridYu, MY=35076866400en_US
dc.identifier.scopusauthoridLahr, G=6701321960en_US
dc.identifier.scopusauthoridChung, TKH=8866217800en_US

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