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Article: Growth and function of isolated canine pancreatic ductal cells

TitleGrowth and function of isolated canine pancreatic ductal cells
Authors
Issue Date2000
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pancreasjournal.com
Citation
Pancreas, 2000, v. 20 n. 1, p. 67-76 How to Cite?
AbstractThese studies investigated the growth characteristics and functional properties of isolated canine pancreatic ductal epithelial cells. Cells were isolated from the accessory pancreatic duct and cultured by using three conditions: on vitrogen-coated petri dishes with fibroblast conditioned medium (nonpolarized); in vitrogen-coated Transwells above a fibroblast feeder layer (polarized); or as organotypic rafts above a fibroblast-embedded collagen layer (polarized). Growth characteristics, transepithelial resistances, and carbonic anhydrase and cyclic adenosine monophosphate (AMP) responses were evaluated. Under polarized conditions, the cells grew as monolayers with columnar epithelial characteristics. The monolayers developed high transepithelial resistance and became impervious to the passage of horseradish peroxidase. Epithelial growth factor (EGF) (2 ng/ml) stimulated ductal cell growth and accelerated the formation of a high-resistance monolayer. Forskolin (10 μM) rapidly decreased transepithelial resistance. Carbonic anhydrase activity, which was lower in nonpolarized compared with polarized conditions, was stimulated by carbachol (175 μM). Secretin, however, did not stimulate carbonic anhydrase activity in these cells. Although secretin stimulated adenylyl cyclase activity in early-passage cells, this response was lost in later-passage cells. Both vasoactive intestinal polypeptide (VIP; 1 μM) and forskolin (10 μM) consistently increased adenylyl cyclase activity. Isolated canine pancreatic ductal epithelial cells proliferate in vitro, develop high-resistance epithelial monolayers, and respond to stimuli that activate adenylyl cyclase. These cells should provide a useful model for regulatory studies of ductal cell functions.
Persistent Identifierhttp://hdl.handle.net/10722/175820
ISSN
2015 Impact Factor: 2.738
2015 SCImago Journal Rankings: 1.114
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Men_US
dc.contributor.authorSchleicher, RLen_US
dc.contributor.authorFink, ASen_US
dc.contributor.authorGunterSmith, Pen_US
dc.contributor.authorSavard, Cen_US
dc.contributor.authorNguyen, Ten_US
dc.contributor.authorLee, SPen_US
dc.date.accessioned2012-11-26T09:01:34Z-
dc.date.available2012-11-26T09:01:34Z-
dc.date.issued2000en_US
dc.identifier.citationPancreas, 2000, v. 20 n. 1, p. 67-76en_US
dc.identifier.issn0885-3177en_US
dc.identifier.urihttp://hdl.handle.net/10722/175820-
dc.description.abstractThese studies investigated the growth characteristics and functional properties of isolated canine pancreatic ductal epithelial cells. Cells were isolated from the accessory pancreatic duct and cultured by using three conditions: on vitrogen-coated petri dishes with fibroblast conditioned medium (nonpolarized); in vitrogen-coated Transwells above a fibroblast feeder layer (polarized); or as organotypic rafts above a fibroblast-embedded collagen layer (polarized). Growth characteristics, transepithelial resistances, and carbonic anhydrase and cyclic adenosine monophosphate (AMP) responses were evaluated. Under polarized conditions, the cells grew as monolayers with columnar epithelial characteristics. The monolayers developed high transepithelial resistance and became impervious to the passage of horseradish peroxidase. Epithelial growth factor (EGF) (2 ng/ml) stimulated ductal cell growth and accelerated the formation of a high-resistance monolayer. Forskolin (10 μM) rapidly decreased transepithelial resistance. Carbonic anhydrase activity, which was lower in nonpolarized compared with polarized conditions, was stimulated by carbachol (175 μM). Secretin, however, did not stimulate carbonic anhydrase activity in these cells. Although secretin stimulated adenylyl cyclase activity in early-passage cells, this response was lost in later-passage cells. Both vasoactive intestinal polypeptide (VIP; 1 μM) and forskolin (10 μM) consistently increased adenylyl cyclase activity. Isolated canine pancreatic ductal epithelial cells proliferate in vitro, develop high-resistance epithelial monolayers, and respond to stimuli that activate adenylyl cyclase. These cells should provide a useful model for regulatory studies of ductal cell functions.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pancreasjournal.comen_US
dc.relation.ispartofPancreasen_US
dc.subject.meshAdenylate Cyclase - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBicarbonates - Metabolismen_US
dc.subject.meshCarbachol - Pharmacologyen_US
dc.subject.meshCarbonic Anhydrases - Metabolismen_US
dc.subject.meshCell Culture Techniques - Instrumentation - Methodsen_US
dc.subject.meshCell Division - Drug Effectsen_US
dc.subject.meshCell Membrane Permeability - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCulture Media, Conditioned - Pharmacologyen_US
dc.subject.meshCyclic Amp - Pharmacologyen_US
dc.subject.meshDogsen_US
dc.subject.meshElectric Impedanceen_US
dc.subject.meshEnzyme Activation - Drug Effectsen_US
dc.subject.meshEpidermal Growth Factor - Pharmacologyen_US
dc.subject.meshEpithelial Cells - Drug Effects - Enzymologyen_US
dc.subject.meshFibroblasts - Secretionen_US
dc.subject.meshForskolin - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshPancreatic Ducts - Cytology - Drug Effects - Enzymologyen_US
dc.subject.meshSecretin - Pharmacologyen_US
dc.subject.meshVasoactive Intestinal Peptide - Pharmacologyen_US
dc.titleGrowth and function of isolated canine pancreatic ductal cellsen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/00006676-200001000-00010en_US
dc.identifier.pmid10630386-
dc.identifier.scopuseid_2-s2.0-0033980372en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033980372&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume20en_US
dc.identifier.issue1en_US
dc.identifier.spage67en_US
dc.identifier.epage76en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZhang, M=7601557136en_US
dc.identifier.scopusauthoridSchleicher, RL=35461049900en_US
dc.identifier.scopusauthoridFink, AS=7202322385en_US
dc.identifier.scopusauthoridGunterSmith, P=6602735292en_US
dc.identifier.scopusauthoridSavard, C=6701738621en_US
dc.identifier.scopusauthoridNguyen, T=35546959700en_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US

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