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Article: Accuracy and sensitivity of DNA pooling with microsatellite repeats using capillary electrophoresis

TitleAccuracy and sensitivity of DNA pooling with microsatellite repeats using capillary electrophoresis
Authors
Issue Date1999
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ymcpr
Citation
Molecular And Cellular Probes, 1999, v. 13 n. 5, p. 359-365 How to Cite?
AbstractDNA pooling is a genetic screening method that combines DNA from many individuals in a single polymerase chain reaction (PCR) reaction to generate a representation of allele frequencies. The substantial saving in effort with DNA pooling over individual genotyping facilitates linkage disequilibrium scanning of the human genome using many thousands of genetic markers, and is applicable to mapping of complex diseases such as schizophrenia. However, the literature to date has not addressed several crucial technical aspects of DNA pooling. These include: DNA quantification; the choice of electrophoresis methods; sensitivity (the minimum reliably detectable difference between pools); and methods of dealing with 'plus-A' stutter. We have examined these points and make recommendations as to the best procedures to adopt as well as quantifying reproducibility and sensitivity. We conclude that, although allele frequencies derived from microsatellite pooling are distorted, differences of 5% or greater between pools can be reliably detected.
Persistent Identifierhttp://hdl.handle.net/10722/175801
ISSN
2015 Impact Factor: 1.565
2015 SCImago Journal Rankings: 0.610
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorBreen, Gen_US
dc.contributor.authorSham, Pen_US
dc.contributor.authorLi, Ten_US
dc.contributor.authorShaw, Den_US
dc.contributor.authorCollier, DAen_US
dc.contributor.authorSt Clair, Den_US
dc.date.accessioned2012-11-26T09:01:25Z-
dc.date.available2012-11-26T09:01:25Z-
dc.date.issued1999en_US
dc.identifier.citationMolecular And Cellular Probes, 1999, v. 13 n. 5, p. 359-365en_US
dc.identifier.issn0890-8508en_US
dc.identifier.urihttp://hdl.handle.net/10722/175801-
dc.description.abstractDNA pooling is a genetic screening method that combines DNA from many individuals in a single polymerase chain reaction (PCR) reaction to generate a representation of allele frequencies. The substantial saving in effort with DNA pooling over individual genotyping facilitates linkage disequilibrium scanning of the human genome using many thousands of genetic markers, and is applicable to mapping of complex diseases such as schizophrenia. However, the literature to date has not addressed several crucial technical aspects of DNA pooling. These include: DNA quantification; the choice of electrophoresis methods; sensitivity (the minimum reliably detectable difference between pools); and methods of dealing with 'plus-A' stutter. We have examined these points and make recommendations as to the best procedures to adopt as well as quantifying reproducibility and sensitivity. We conclude that, although allele frequencies derived from microsatellite pooling are distorted, differences of 5% or greater between pools can be reliably detected.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ymcpren_US
dc.relation.ispartofMolecular and Cellular Probesen_US
dc.subject.meshAllelesen_US
dc.subject.meshDna - Blooden_US
dc.subject.meshElectrophoresis, Capillary - Methodsen_US
dc.subject.meshFluorometryen_US
dc.subject.meshGenetic Testing - Economics - Methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshMicrosatellite Repeats - Geneticsen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSpectrophotometryen_US
dc.titleAccuracy and sensitivity of DNA pooling with microsatellite repeats using capillary electrophoresisen_US
dc.typeArticleen_US
dc.identifier.emailSham, P: pcsham@hku.hken_US
dc.identifier.authoritySham, P=rp00459en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/mcpr.1999.0259en_US
dc.identifier.pmid10508557-
dc.identifier.scopuseid_2-s2.0-0032879023en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032879023&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume13en_US
dc.identifier.issue5en_US
dc.identifier.spage359en_US
dc.identifier.epage365en_US
dc.identifier.isiWOS:000083117500004-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridBreen, G=15742166000en_US
dc.identifier.scopusauthoridSham, P=34573429300en_US
dc.identifier.scopusauthoridLi, T=36168387000en_US
dc.identifier.scopusauthoridShaw, D=7403341741en_US
dc.identifier.scopusauthoridCollier, DA=26642980600en_US
dc.identifier.scopusauthoridSt Clair, D=35354078200en_US

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