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Article: Bile salts stimulate mucin secretion by cultured dog gallbladder epithelial cells independent of their detergent effect

TitleBile salts stimulate mucin secretion by cultured dog gallbladder epithelial cells independent of their detergent effect
Authors
Issue Date1998
PublisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.org
Citation
Biochemical Journal, 1998, v. 332 n. 1, p. 257-262 How to Cite?
Abstract1. Bile salts stimulate mucin secretion by the gallbladder epithelium. We have investigated whether this stimulatory effect is due to a detergent effect of bile salts. 2. The bile salts taurocholic acid (TC) and tauroursodeoxycholic acid (TUDC) and the detergents Triton X-100 (12.5-400 μM) and Tween-20 (0.1-3.2 mM) were applied to monolayers of cultured dog gallbladder epithelial cells. Mucin secretion was studied by measuring the secretion of [3H]N-acetyl-D-glucosamine-labelled glycoproteins. We also attempted to alter the fluidity of the apical membrane of the cells through extraction of cholesterol with β-cyclodextrin (2.5-15 mM). The effect on TUDC-induced mucin secretion was studied. Cell viability was assessed by measuring lactate dehydrogenase (LDH) leakage or 51Cr release. 3. In contrast with the bile salts, the detergents were not able to cause an increase in mucin secretion without causing concomitant cell lysis. Concentrations of detergent that increased mucin release (> 100 μM Triton X-100, > 0.8 mM Tween-20), caused increased LDH release. Incubation with β-cyclodextrin resulted in effective extraction of cholesterol without causing an increase in 51Cr release. However, no effect of the presumed altered membrane fluidity on TUDC (10 mM)-induced mucin secretion was observed. 4. The stimulatory effect of bile salts on mucin secretion by gallbladder epithelial cells is not affected by the fluidity of the apical membrane of the cells and also cannot be mimicked by other detergents. We conclude that the ability of bile salts to cause mucin secretion by the gallbladder epithelium is not determined by their detergent properties.
Persistent Identifierhttp://hdl.handle.net/10722/175792
ISSN
2015 Impact Factor: 3.562
2015 SCImago Journal Rankings: 2.582
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKlinkspoor, JHen_US
dc.contributor.authorYoshida, Ten_US
dc.contributor.authorLee, SPen_US
dc.date.accessioned2012-11-26T09:01:19Z-
dc.date.available2012-11-26T09:01:19Z-
dc.date.issued1998en_US
dc.identifier.citationBiochemical Journal, 1998, v. 332 n. 1, p. 257-262en_US
dc.identifier.issn0264-6021en_US
dc.identifier.urihttp://hdl.handle.net/10722/175792-
dc.description.abstract1. Bile salts stimulate mucin secretion by the gallbladder epithelium. We have investigated whether this stimulatory effect is due to a detergent effect of bile salts. 2. The bile salts taurocholic acid (TC) and tauroursodeoxycholic acid (TUDC) and the detergents Triton X-100 (12.5-400 μM) and Tween-20 (0.1-3.2 mM) were applied to monolayers of cultured dog gallbladder epithelial cells. Mucin secretion was studied by measuring the secretion of [3H]N-acetyl-D-glucosamine-labelled glycoproteins. We also attempted to alter the fluidity of the apical membrane of the cells through extraction of cholesterol with β-cyclodextrin (2.5-15 mM). The effect on TUDC-induced mucin secretion was studied. Cell viability was assessed by measuring lactate dehydrogenase (LDH) leakage or 51Cr release. 3. In contrast with the bile salts, the detergents were not able to cause an increase in mucin secretion without causing concomitant cell lysis. Concentrations of detergent that increased mucin release (> 100 μM Triton X-100, > 0.8 mM Tween-20), caused increased LDH release. Incubation with β-cyclodextrin resulted in effective extraction of cholesterol without causing an increase in 51Cr release. However, no effect of the presumed altered membrane fluidity on TUDC (10 mM)-induced mucin secretion was observed. 4. The stimulatory effect of bile salts on mucin secretion by gallbladder epithelial cells is not affected by the fluidity of the apical membrane of the cells and also cannot be mimicked by other detergents. We conclude that the ability of bile salts to cause mucin secretion by the gallbladder epithelium is not determined by their detergent properties.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.orgen_US
dc.relation.ispartofBiochemical Journalen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBile Acids And Salts - Pharmacologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCholesterol - Metabolismen_US
dc.subject.meshCyclodextrins - Pharmacologyen_US
dc.subject.meshDetergents - Pharmacologyen_US
dc.subject.meshDogsen_US
dc.subject.meshGallbladder - Drug Effectsen_US
dc.subject.meshGlycoproteins - Secretionen_US
dc.subject.meshMembrane Fluidity - Drug Effects - Physiologyen_US
dc.subject.meshMucins - Secretionen_US
dc.subject.meshOctoxynol - Pharmacologyen_US
dc.subject.meshPolysorbates - Pharmacologyen_US
dc.subject.meshTaurochenodeoxycholic Acid - Pharmacologyen_US
dc.subject.meshTaurocholic Acid - Pharmacologyen_US
dc.subject.meshBeta-Cyclodextrinsen_US
dc.titleBile salts stimulate mucin secretion by cultured dog gallbladder epithelial cells independent of their detergent effecten_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9576876-
dc.identifier.scopuseid_2-s2.0-0032524858en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032524858&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume332en_US
dc.identifier.issue1en_US
dc.identifier.spage257en_US
dc.identifier.epage262en_US
dc.identifier.isiWOS:000073881900033-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridKlinkspoor, JH=6602590656en_US
dc.identifier.scopusauthoridYoshida, T=7501317704en_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US

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