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Article: Constitutive mucin secretion linked to CFTR expression

TitleConstitutive mucin secretion linked to CFTR expression
Authors
Issue Date1994
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 1994, v. 203 n. 3, p. 1457-1462 How to Cite?
AbstractMucus plugging is a hallmark of cystic fibrosis, but the link between the defective gene product, the cystic fibrosis transmembrane conductance regulator, and the abnormal mucus phenotype is unclear. To demonstrate CFTR involvement in mucin glycoprotein secretion in epithelial cells, a retroviral vector was used to overexpress CFTR in gallbaldder epithelial cells, and constitutive mucin labeling and secretion were monitored. Achievement of high-level vector expression was confirmed by transduction with marker genes. Cells transduced with vectors carrying CFTR cDNA showed 5-fold increased expression of CFTR by Western blotting. Mucin labeling and secretion were 4-fold elevated in transduced cells. These results suggest constitutive mucin synthesis and secretion in gallbladder epithelial cells are regulated by CFTR.
Persistent Identifierhttp://hdl.handle.net/10722/175695
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKuver, Ren_US
dc.contributor.authorRamesh, Nen_US
dc.contributor.authorLau, Sen_US
dc.contributor.authorSavard, Cen_US
dc.contributor.authorLee, SPen_US
dc.contributor.authorOsborne, WRAen_US
dc.date.accessioned2012-11-26T09:00:33Z-
dc.date.available2012-11-26T09:00:33Z-
dc.date.issued1994en_US
dc.identifier.citationBiochemical And Biophysical Research Communications, 1994, v. 203 n. 3, p. 1457-1462en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/175695-
dc.description.abstractMucus plugging is a hallmark of cystic fibrosis, but the link between the defective gene product, the cystic fibrosis transmembrane conductance regulator, and the abnormal mucus phenotype is unclear. To demonstrate CFTR involvement in mucin glycoprotein secretion in epithelial cells, a retroviral vector was used to overexpress CFTR in gallbaldder epithelial cells, and constitutive mucin labeling and secretion were monitored. Achievement of high-level vector expression was confirmed by transduction with marker genes. Cells transduced with vectors carrying CFTR cDNA showed 5-fold increased expression of CFTR by Western blotting. Mucin labeling and secretion were 4-fold elevated in transduced cells. These results suggest constitutive mucin synthesis and secretion in gallbladder epithelial cells are regulated by CFTR.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodiesen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCell Lineen_US
dc.subject.meshChloride Channels - Biosynthesisen_US
dc.subject.meshCystic Fibrosis Transmembrane Conductance Regulatoren_US
dc.subject.meshDna, Complementary - Metabolismen_US
dc.subject.meshDogsen_US
dc.subject.meshEpithelium - Metabolismen_US
dc.subject.meshGallbladder - Metabolismen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshHomeostasisen_US
dc.subject.meshHumansen_US
dc.subject.meshKineticsen_US
dc.subject.meshMembrane Proteins - Analysis - Biosynthesisen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMucins - Biosynthesis - Secretionen_US
dc.subject.meshOligopeptides - Chemistry - Immunologyen_US
dc.subject.meshTransfectionen_US
dc.titleConstitutive mucin secretion linked to CFTR expressionen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/bbrc.1994.2348en_US
dc.identifier.pmid7524485-
dc.identifier.scopuseid_2-s2.0-0028061411en_US
dc.identifier.volume203en_US
dc.identifier.issue3en_US
dc.identifier.spage1457en_US
dc.identifier.epage1462en_US
dc.identifier.isiWOS:A1994PJ52600014-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKuver, R=6701723533en_US
dc.identifier.scopusauthoridRamesh, N=24424482700en_US
dc.identifier.scopusauthoridLau, S=7401596194en_US
dc.identifier.scopusauthoridSavard, C=6701738621en_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US
dc.identifier.scopusauthoridOsborne, WRA=7005020110en_US

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