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Article: Isolation and partial characterization of a soluble mucin in human pancreatic juice.

TitleIsolation and partial characterization of a soluble mucin in human pancreatic juice.
Authors
Issue Date1991
PublisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?bcb
Citation
Biochemistry And Cell Biology = Biochimie Et Biologie Cellulaire, 1991, v. 69 n. 8, p. 566-571 How to Cite?
AbstractMorphological and histochemical abnormalities in pancreatic mucin occur in many pancreatic disorders. However, the composition of pancreatic mucin is poorly understood. Purified mucin was isolated from pure pancreatic juice by sequential chromatography on Sepharose CL-2B and CL-4B followed by CsCl density gradient ultracentrifugation. The mucin preparation consists of 24% protein and 73% carbohydrate. Reduction of the macromolecule (greater than 2 x 10(6)) by mercaptoethanol resulted in the formation of subunits of molecular weight 500,000 and released several small molecular weight proteins, including a glycoprotein of an average molecular weight of 116,000. Cellulose acetate electrophoresis separated the mucin into three species of different staining properties for periodic acid-Schiff reagent and Alcian blue, suggesting the presence of microheterogeneity with respect to sulphation and sialation. Threonine, serine, and proline composed 48% of the total amino acids, while the oligosaccharide moiety contained N-acetylglucosamine, N-acetylgalactosamine, fucose, galactose, sialic acid, and sulphate. We also detected the presence of C16:0 and C18:0 fatty acids which were probably noncovalently bound to the pancreatic mucin.
Persistent Identifierhttp://hdl.handle.net/10722/175667
ISSN
2015 Impact Factor: 1.527
2015 SCImago Journal Rankings: 0.859
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, SPen_US
dc.contributor.authorChoong, YSen_US
dc.contributor.authorPark, HZen_US
dc.date.accessioned2012-11-26T09:00:22Z-
dc.date.available2012-11-26T09:00:22Z-
dc.date.issued1991en_US
dc.identifier.citationBiochemistry And Cell Biology = Biochimie Et Biologie Cellulaire, 1991, v. 69 n. 8, p. 566-571en_US
dc.identifier.issn0829-8211en_US
dc.identifier.urihttp://hdl.handle.net/10722/175667-
dc.description.abstractMorphological and histochemical abnormalities in pancreatic mucin occur in many pancreatic disorders. However, the composition of pancreatic mucin is poorly understood. Purified mucin was isolated from pure pancreatic juice by sequential chromatography on Sepharose CL-2B and CL-4B followed by CsCl density gradient ultracentrifugation. The mucin preparation consists of 24% protein and 73% carbohydrate. Reduction of the macromolecule (greater than 2 x 10(6)) by mercaptoethanol resulted in the formation of subunits of molecular weight 500,000 and released several small molecular weight proteins, including a glycoprotein of an average molecular weight of 116,000. Cellulose acetate electrophoresis separated the mucin into three species of different staining properties for periodic acid-Schiff reagent and Alcian blue, suggesting the presence of microheterogeneity with respect to sulphation and sialation. Threonine, serine, and proline composed 48% of the total amino acids, while the oligosaccharide moiety contained N-acetylglucosamine, N-acetylgalactosamine, fucose, galactose, sialic acid, and sulphate. We also detected the presence of C16:0 and C18:0 fatty acids which were probably noncovalently bound to the pancreatic mucin.en_US
dc.languageengen_US
dc.publisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?bcben_US
dc.relation.ispartofBiochemistry and cell biology = Biochimie et biologie cellulaireen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAmino Acids - Analysisen_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshElectrophoresis, Cellulose Acetateen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMonosaccharides - Analysisen_US
dc.subject.meshMucins - Chemistry - Isolation & Purificationen_US
dc.subject.meshPancreatic Juice - Chemistryen_US
dc.subject.meshSulfates - Analysisen_US
dc.titleIsolation and partial characterization of a soluble mucin in human pancreatic juice.en_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1139/o91-084-
dc.identifier.pmid1760160-
dc.identifier.scopuseid_2-s2.0-0026202057en_US
dc.identifier.volume69en_US
dc.identifier.issue8en_US
dc.identifier.spage566en_US
dc.identifier.epage571en_US
dc.identifier.isiWOS:A1991GF82400011-
dc.publisher.placeCanadaen_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US
dc.identifier.scopusauthoridChoong, YS=36797592600en_US
dc.identifier.scopusauthoridPark, HZ=7601570519en_US

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