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Article: Interconversions of lipid aggregates in rat and model bile

TitleInterconversions of lipid aggregates in rat and model bile
Authors
Issue Date1991
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1991, v. 260 n. 1 23-1, p. G70-G79 How to Cite?
AbstractThe dynamic interchange of cholesterol and the phase transition between nonmicellar and micellar aggregates in rat and model bile were characterized with gel-permeation chromatography, quasi-elastic light scattering, turbidity measurements, and by radiolabeling lipid aggregates in bile. Cholesterol partitioned into either the micellar or nonmicellar phases independent of the lipid aggregate structure. In model bile, increasing bile salt concentrations led to a decrease in the relative proportion of nonmicellar aggregates beginning at 5 mM taurocholate (TC), while the relative cholesterol content of the nonmicellar fraction increased from 1.0 to 2.7 ± 2.0 (means ± SD). In rats, creation of a biliary fistula resulted in a decrease of bile salts from 41 to 4 mM. Mixed micelles increased from 25 to 120 AÅ in radius, while nonmicellar aggregates increased from 180 to 800 AÅ in radius. Addition of TC to model bile (cholesterol:lecithin = 1:1) vesicles with total lipid concentrations <7 mM yielded a progressive shift of vesicles (450 AÅ) to mixed micelles (30 AÅ). For mixtures with higher total lipid concentrations, addition of TC promoted substantial vesicle aggregation and resulted in formation of a third phase containing lipid aggregates larger in size than the initial vesicles. These results suggest that rapid exchange of cholesterol occurs in bile and that significant remodeling of vesicles can occur. These alterations in vesicles include both enrichment in cholesterol content and formation of larger aggregates during increases in bile salt concentration.
Persistent Identifierhttp://hdl.handle.net/10722/175666
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLittle, TEen_US
dc.contributor.authorLee, SPen_US
dc.contributor.authorMadani, Hen_US
dc.contributor.authorKaler, EWen_US
dc.contributor.authorChinn, Ken_US
dc.date.accessioned2012-11-26T09:00:21Z-
dc.date.available2012-11-26T09:00:21Z-
dc.date.issued1991en_US
dc.identifier.citationAmerican Journal Of Physiology - Gastrointestinal And Liver Physiology, 1991, v. 260 n. 1 23-1, p. G70-G79en_US
dc.identifier.issn0002-9513en_US
dc.identifier.urihttp://hdl.handle.net/10722/175666-
dc.description.abstractThe dynamic interchange of cholesterol and the phase transition between nonmicellar and micellar aggregates in rat and model bile were characterized with gel-permeation chromatography, quasi-elastic light scattering, turbidity measurements, and by radiolabeling lipid aggregates in bile. Cholesterol partitioned into either the micellar or nonmicellar phases independent of the lipid aggregate structure. In model bile, increasing bile salt concentrations led to a decrease in the relative proportion of nonmicellar aggregates beginning at 5 mM taurocholate (TC), while the relative cholesterol content of the nonmicellar fraction increased from 1.0 to 2.7 ± 2.0 (means ± SD). In rats, creation of a biliary fistula resulted in a decrease of bile salts from 41 to 4 mM. Mixed micelles increased from 25 to 120 AÅ in radius, while nonmicellar aggregates increased from 180 to 800 AÅ in radius. Addition of TC to model bile (cholesterol:lecithin = 1:1) vesicles with total lipid concentrations <7 mM yielded a progressive shift of vesicles (450 AÅ) to mixed micelles (30 AÅ). For mixtures with higher total lipid concentrations, addition of TC promoted substantial vesicle aggregation and resulted in formation of a third phase containing lipid aggregates larger in size than the initial vesicles. These results suggest that rapid exchange of cholesterol occurs in bile and that significant remodeling of vesicles can occur. These alterations in vesicles include both enrichment in cholesterol content and formation of larger aggregates during increases in bile salt concentration.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Gastrointestinal and Liver Physiologyen_US
dc.subject.mesh1,2-Dipalmitoylphosphatidylcholine - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBile - Physiologyen_US
dc.subject.meshCholesterol - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshMicellesen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshMolecular Conformationen_US
dc.subject.meshPhospholipids - Metabolismen_US
dc.subject.meshRatsen_US
dc.titleInterconversions of lipid aggregates in rat and model bileen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1987810-
dc.identifier.scopuseid_2-s2.0-0026060856en_US
dc.identifier.volume260en_US
dc.identifier.issue1 23-1en_US
dc.identifier.spageG70en_US
dc.identifier.epageG79en_US
dc.identifier.isiWOS:A1991EU06500055-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLittle, TE=35822432000en_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US
dc.identifier.scopusauthoridMadani, H=6701613342en_US
dc.identifier.scopusauthoridKaler, EW=7007157989en_US
dc.identifier.scopusauthoridChinn, K=36896420800en_US

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