File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Diffusion of butyrate through pig colonic mucus in vitro

TitleDiffusion of butyrate through pig colonic mucus in vitro
Authors
Issue Date1986
PublisherPortland Press Ltd. The Journal's web site is located at http://www.clinsci.org/
Citation
Clinical Science, 1986, v. 70 n. 3, p. 271-276 How to Cite?
AbstractUsing a modified equilibrium dialysis cell the rate of diffusion of butyrate through pig colonic mucus has been compared with that through other gels and unstirred layers. Relative diffusion coefficients were calculated for each layer. Layers of 8% polyacrylamide, and of caecal, mid-colonic and terminal colonic mucus, had coefficients that were 50-60% of the apparent free diffusion coefficient for butyrate, determined using layers made up of Millipore filters alone. The apparent free diffusion coefficients for butyrate (layers of agarose or filters) were 70% of previously determined values in the literature. This discrepancy can be explained by elements of the experimental procedure. All mucus layers differed significantly from layers of 2% agarose and Millipore filters but were not significantly different from layers of 8% polyacrylamide or from each other. Diffusion coefficients for butyrate in the mucus samples correlated with water content and carbohydrate content but had no relationship to protein content. The rate of diffusion of butyrate in colonic mucus layers was significantly reduced when compared with unstirred layers (P < 0.05). Whether this has an effect on the butyrate supply to colonocytes in vitro and whether mucus in colonic disease behaves differently are subjects for further investigation.
Persistent Identifierhttp://hdl.handle.net/10722/175641
ISSN
2015 Impact Factor: 4.996
2015 SCImago Journal Rankings: 2.427
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSmith, GWen_US
dc.contributor.authorWiggins, PMen_US
dc.contributor.authorLee, SPen_US
dc.contributor.authorTasmanJones, Cen_US
dc.date.accessioned2012-11-26T09:00:14Z-
dc.date.available2012-11-26T09:00:14Z-
dc.date.issued1986en_US
dc.identifier.citationClinical Science, 1986, v. 70 n. 3, p. 271-276en_US
dc.identifier.issn0143-5221en_US
dc.identifier.urihttp://hdl.handle.net/10722/175641-
dc.description.abstractUsing a modified equilibrium dialysis cell the rate of diffusion of butyrate through pig colonic mucus has been compared with that through other gels and unstirred layers. Relative diffusion coefficients were calculated for each layer. Layers of 8% polyacrylamide, and of caecal, mid-colonic and terminal colonic mucus, had coefficients that were 50-60% of the apparent free diffusion coefficient for butyrate, determined using layers made up of Millipore filters alone. The apparent free diffusion coefficients for butyrate (layers of agarose or filters) were 70% of previously determined values in the literature. This discrepancy can be explained by elements of the experimental procedure. All mucus layers differed significantly from layers of 2% agarose and Millipore filters but were not significantly different from layers of 8% polyacrylamide or from each other. Diffusion coefficients for butyrate in the mucus samples correlated with water content and carbohydrate content but had no relationship to protein content. The rate of diffusion of butyrate in colonic mucus layers was significantly reduced when compared with unstirred layers (P < 0.05). Whether this has an effect on the butyrate supply to colonocytes in vitro and whether mucus in colonic disease behaves differently are subjects for further investigation.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.clinsci.org/en_US
dc.relation.ispartofClinical Scienceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshButyrates - Metabolismen_US
dc.subject.meshButyric Aciden_US
dc.subject.meshButyric Acids - Metabolismen_US
dc.subject.meshColon - Metabolismen_US
dc.subject.meshDiffusionen_US
dc.subject.meshIntestinal Mucosa - Metabolismen_US
dc.subject.meshMucus - Analysis - Metabolismen_US
dc.subject.meshSwineen_US
dc.titleDiffusion of butyrate through pig colonic mucus in vitroen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3948476-
dc.identifier.scopuseid_2-s2.0-0022588074en_US
dc.identifier.volume70en_US
dc.identifier.issue3en_US
dc.identifier.spage271en_US
dc.identifier.epage276en_US
dc.identifier.isiWOS:A1986A401300008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridSmith, GW=7406739744en_US
dc.identifier.scopusauthoridWiggins, PM=7006787396en_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US
dc.identifier.scopusauthoridTasmanJones, C=7003303326en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats