File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The degradation of 5'-deoxy-5-fluorouridine by pyrimidine nucleoside phosphorylase in normal and cancer tissues

TitleThe degradation of 5'-deoxy-5-fluorouridine by pyrimidine nucleoside phosphorylase in normal and cancer tissues
Authors
Issue Date1985
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/cca
Citation
Clinica Chimica Acta, 1985, v. 149 n. 2-3, p. 175-183 How to Cite?
AbstractWe describe an assay procedure for the quantification of pyrimidine nucleoside phosphorylase, the enzyme thought to degrade the pro-drug 5'-deoxy-5-fluorouridine to 5-fluorouracil. The method is based on the known differences in the ultraviolet absorption spectra in alkaline medium between pyrimidines and their nucleosides. Analogous to the cleavage of uridine by uridine phosphorylase, the enzymatic degradation of 5'-deoxy-5-fluorouridine in the presence of inorganic phosphate yields 5-fluorouracil and ribose-1-phosphate. We have shown that the rate of phosphorolysis of this pyrimidine nucleoside could be readily measured by spectrophotometry. The method described is simple, specific for the 'pro-drug' as well as reproducible. We have also applied this method to define tissue enzyme activities in extracts of human tumours, normal tissues of the same organ and tissues of mice.
Persistent Identifierhttp://hdl.handle.net/10722/175635
ISSN
2015 Impact Factor: 2.799
2015 SCImago Journal Rankings: 1.040
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChoong, YSen_US
dc.contributor.authorLee, SPen_US
dc.date.accessioned2012-11-26T09:00:12Z-
dc.date.available2012-11-26T09:00:12Z-
dc.date.issued1985en_US
dc.identifier.citationClinica Chimica Acta, 1985, v. 149 n. 2-3, p. 175-183en_US
dc.identifier.issn0009-8981en_US
dc.identifier.urihttp://hdl.handle.net/10722/175635-
dc.description.abstractWe describe an assay procedure for the quantification of pyrimidine nucleoside phosphorylase, the enzyme thought to degrade the pro-drug 5'-deoxy-5-fluorouridine to 5-fluorouracil. The method is based on the known differences in the ultraviolet absorption spectra in alkaline medium between pyrimidines and their nucleosides. Analogous to the cleavage of uridine by uridine phosphorylase, the enzymatic degradation of 5'-deoxy-5-fluorouridine in the presence of inorganic phosphate yields 5-fluorouracil and ribose-1-phosphate. We have shown that the rate of phosphorolysis of this pyrimidine nucleoside could be readily measured by spectrophotometry. The method described is simple, specific for the 'pro-drug' as well as reproducible. We have also applied this method to define tissue enzyme activities in extracts of human tumours, normal tissues of the same organ and tissues of mice.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ccaen_US
dc.relation.ispartofClinica Chimica Actaen_US
dc.subject.meshAnimalsen_US
dc.subject.meshColon - Enzymologyen_US
dc.subject.meshFloxuridine - Metabolismen_US
dc.subject.meshFluorouracil - Metabolismen_US
dc.subject.meshGastrointestinal Neoplasms - Enzymologyen_US
dc.subject.meshHumansen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshIntestine, Small - Enzymologyen_US
dc.subject.meshKineticsen_US
dc.subject.meshLymph Nodes - Enzymologyen_US
dc.subject.meshMiceen_US
dc.subject.meshPentosyltransferases - Metabolismen_US
dc.subject.meshPyrimidine Phosphorylasesen_US
dc.subject.meshSpectrophotometry, Ultravioleten_US
dc.subject.meshTissue Distributionen_US
dc.titleThe degradation of 5'-deoxy-5-fluorouridine by pyrimidine nucleoside phosphorylase in normal and cancer tissuesen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0009-8981(85)90331-6-
dc.identifier.pmid3161668-
dc.identifier.scopuseid_2-s2.0-0021871124en_US
dc.identifier.volume149en_US
dc.identifier.issue2-3en_US
dc.identifier.spage175en_US
dc.identifier.epage183en_US
dc.identifier.isiWOS:A1985APD1300008-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChoong, YS=36797593200en_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats