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Article: Toxin-induced cell membrane injury in guinea pigs given lincomycin

TitleToxin-induced cell membrane injury in guinea pigs given lincomycin
Authors
Issue Date1982
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00313025.asp
Citation
Pathology, 1982, v. 14 n. 3, p. 317-322 How to Cite?
AbstractGuinea pigs treated with lincomycin developed colitis, acute cholecystitis and abnormalities in red blood cell morphology. The present study was designed to study the production of clostridial toxins after lincomycin treatment. Lincomycin produced abnormalities in conventional but not in germ-free guinea pigs. Clostridium difficile was cultured from cecal contents of conventional guinea pigs treated with lincomycin. Cecal filtrate from sick guinea pigs was subjected to Sepharose 4B-CL and Sephadex G-200 column chromatography, yielding a partially purified toxin. Both cecal filtrate and partially purified toxin samples contained a heat labile substance which was cytotoxic to human lung fibroblast WI-38 cells, and which was neutralized by Clostridium sordelli antitoxin. Toxin samples given orally or intraperitoneally killed normal guinea pigs. Finally, toxin samples induced red cell membrane changes in vitro as well as producing features of acute inflammation in healthy explants of guinea pig cecum and gallbladder in organ culture. Lincomycin treated guinea pigs produced Clostridum difficile toxins(s) which in turn caused diffuse cell membrane injury.
Persistent Identifierhttp://hdl.handle.net/10722/175627
ISSN
2015 Impact Factor: 2.968
2015 SCImago Journal Rankings: 1.049
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, SPen_US
dc.contributor.authorThomsen, LLen_US
dc.date.accessioned2012-11-26T09:00:11Z-
dc.date.available2012-11-26T09:00:11Z-
dc.date.issued1982en_US
dc.identifier.citationPathology, 1982, v. 14 n. 3, p. 317-322en_US
dc.identifier.issn0031-3025en_US
dc.identifier.urihttp://hdl.handle.net/10722/175627-
dc.description.abstractGuinea pigs treated with lincomycin developed colitis, acute cholecystitis and abnormalities in red blood cell morphology. The present study was designed to study the production of clostridial toxins after lincomycin treatment. Lincomycin produced abnormalities in conventional but not in germ-free guinea pigs. Clostridium difficile was cultured from cecal contents of conventional guinea pigs treated with lincomycin. Cecal filtrate from sick guinea pigs was subjected to Sepharose 4B-CL and Sephadex G-200 column chromatography, yielding a partially purified toxin. Both cecal filtrate and partially purified toxin samples contained a heat labile substance which was cytotoxic to human lung fibroblast WI-38 cells, and which was neutralized by Clostridium sordelli antitoxin. Toxin samples given orally or intraperitoneally killed normal guinea pigs. Finally, toxin samples induced red cell membrane changes in vitro as well as producing features of acute inflammation in healthy explants of guinea pig cecum and gallbladder in organ culture. Lincomycin treated guinea pigs produced Clostridum difficile toxins(s) which in turn caused diffuse cell membrane injury.en_US
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00313025.aspen_US
dc.relation.ispartofPathologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBacterial Toxins - Pharmacologyen_US
dc.subject.meshCholecystitis - Etiologyen_US
dc.subject.meshCholelithiasis - Etiologyen_US
dc.subject.meshClostridiumen_US
dc.subject.meshEnteritis - Etiologyen_US
dc.subject.meshErythrocyte Membrane - Drug Effects - Injuriesen_US
dc.subject.meshErythrocytes - Drug Effectsen_US
dc.subject.meshGerm-Free Lifeen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshLincomycin - Therapeutic Useen_US
dc.subject.meshToxicology - Methodsen_US
dc.titleToxin-induced cell membrane injury in guinea pigs given lincomycinen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.3109/00313028209061385-
dc.identifier.pmid7133762-
dc.identifier.scopuseid_2-s2.0-0020414466en_US
dc.identifier.volume14en_US
dc.identifier.issue3en_US
dc.identifier.spage317en_US
dc.identifier.epage322en_US
dc.identifier.isiWOS:A1982PF73900016-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US
dc.identifier.scopusauthoridThomsen, LL=55107476400en_US

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