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Article: Carbohydrate moieties of glycoproteins in human hepatic and gall-bladder bile, gall-bladder mucosa and gall stones

TitleCarbohydrate moieties of glycoproteins in human hepatic and gall-bladder bile, gall-bladder mucosa and gall stones
Authors
Issue Date1979
PublisherPortland Press Ltd. The Journal's web site is located at http://www.clinsci.org/
Citation
Clinical Science, 1979, v. 56 n. 6, p. 533-530 How to Cite?
Abstract1. The soluble glycoproteins of human bile, gallbladder mucosa and gall stones have been extracted and hydrolysed, and the monosaccharides analysed by gas-liquid chromatography. 2. Human biliary glycoproteins contained 55-75% of carbohydrate, the major monosaccharide components being galactose, fucose and N-acetylglucosamine, accounting for 70-85% of all the monosaccharides. Mannose, glucose, N-acetylgalactosamine and N-acetylneuraminic acid (sialic acid) were also present. N-Acetylneuraminic acid was present in large amounts in the gall-bladder mucosa and bile of one ulcerated and markedly inflamed gall bladder. 3. The proportion of monosaccharides in soluble glycoproteins of mucosa and bile were not different in samples from subjects with or without gall stones. 4. Gall stones were analysed for cholesterol, calcium and bilirubin and classified as 'cholesterol stones' (7/10) and 'pigment stones' (3/10). Both cholesterol and pigment stones contain a variable amount of glycoprotein. The pattern of carbohydrate consituents was similar to that present in the gall-bladder mucosa and bile in the same subject. There was also no major difference between the pattern found in 'cholesterol' and 'pigment' stones. 5. Evidence and argument are presented suggesting that some glycoprotein is secreted by the gall bladder and incorporated into gall stones. This calls for further work upon the influence of these carbohydrate-rich macromolecules on cholesterol solubilization in mixed micelles.
Persistent Identifierhttp://hdl.handle.net/10722/175609
ISSN
2015 Impact Factor: 4.996
2015 SCImago Journal Rankings: 2.427
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, SPen_US
dc.contributor.authorLim, THen_US
dc.contributor.authorScott, AJen_US
dc.date.accessioned2012-11-26T09:00:07Z-
dc.date.available2012-11-26T09:00:07Z-
dc.date.issued1979en_US
dc.identifier.citationClinical Science, 1979, v. 56 n. 6, p. 533-530en_US
dc.identifier.issn0143-5221en_US
dc.identifier.urihttp://hdl.handle.net/10722/175609-
dc.description.abstract1. The soluble glycoproteins of human bile, gallbladder mucosa and gall stones have been extracted and hydrolysed, and the monosaccharides analysed by gas-liquid chromatography. 2. Human biliary glycoproteins contained 55-75% of carbohydrate, the major monosaccharide components being galactose, fucose and N-acetylglucosamine, accounting for 70-85% of all the monosaccharides. Mannose, glucose, N-acetylgalactosamine and N-acetylneuraminic acid (sialic acid) were also present. N-Acetylneuraminic acid was present in large amounts in the gall-bladder mucosa and bile of one ulcerated and markedly inflamed gall bladder. 3. The proportion of monosaccharides in soluble glycoproteins of mucosa and bile were not different in samples from subjects with or without gall stones. 4. Gall stones were analysed for cholesterol, calcium and bilirubin and classified as 'cholesterol stones' (7/10) and 'pigment stones' (3/10). Both cholesterol and pigment stones contain a variable amount of glycoprotein. The pattern of carbohydrate consituents was similar to that present in the gall-bladder mucosa and bile in the same subject. There was also no major difference between the pattern found in 'cholesterol' and 'pigment' stones. 5. Evidence and argument are presented suggesting that some glycoprotein is secreted by the gall bladder and incorporated into gall stones. This calls for further work upon the influence of these carbohydrate-rich macromolecules on cholesterol solubilization in mixed micelles.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.clinsci.org/en_US
dc.relation.ispartofClinical Scienceen_US
dc.subject.meshAcetylglucosamine - Isolation & Purificationen_US
dc.subject.meshBile - Analysisen_US
dc.subject.meshCholelithiasis - Metabolismen_US
dc.subject.meshFucose - Isolation & Purificationen_US
dc.subject.meshGalactose - Isolation & Purificationen_US
dc.subject.meshGallbladder - Analysisen_US
dc.subject.meshGlycoproteins - Isolation & Purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshLiver - Analysisen_US
dc.subject.meshMonosaccharides - Isolation & Purificationen_US
dc.subject.meshMucous Membrane - Analysisen_US
dc.subject.meshSialic Acids - Isolation & Purificationen_US
dc.titleCarbohydrate moieties of glycoproteins in human hepatic and gall-bladder bile, gall-bladder mucosa and gall stonesen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid477239-
dc.identifier.scopuseid_2-s2.0-0018645420en_US
dc.identifier.volume56en_US
dc.identifier.issue6en_US
dc.identifier.spage533en_US
dc.identifier.epage530en_US
dc.identifier.isiWOS:A1979HB11200003-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US
dc.identifier.scopusauthoridLim, TH=55219829700en_US
dc.identifier.scopusauthoridScott, AJ=7403271310en_US

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