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- Publisher Website: 10.1016/j.jpba.2004.09.050
- Scopus: eid_2-s2.0-12344335164
- PMID: 15664754
- WOS: WOS:000227014200017
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Article: Rapid determination of tramadol in human plasma by headspace solid-phase microextraction and capillary gas chromatography-mass spectrometry
Title | Rapid determination of tramadol in human plasma by headspace solid-phase microextraction and capillary gas chromatography-mass spectrometry |
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Authors | |
Keywords | Gas chromatography-mass spectrometry Headspace solid-phase microextraction Human plasma Tramadol |
Issue Date | 2005 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpba |
Citation | Journal Of Pharmaceutical And Biomedical Analysis, 2005, v. 37 n. 1, p. 143-147 How to Cite? |
Abstract | A simple, rapid and sensitive method for determination of tramadol in plasma samples was developed using headspace solid-phase microextraction (HS-SPME) and gas chromatography with mass spectrometry (GC-MS). The optimum conditions for the SPME procedure were: headspace extraction on a 65-μm polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber; 0.5 mL of plasma modified with 0.5 mL of sodium hydroxide (0.1 M); extraction temperature of 100°C, with stirring at 2000 rpm for 30 min. The calibration curve showed linearity in the range of 1-400 ng mL-1 with regression coefficient corresponding to 0.9986 and coefficient of the variation of the points of the calibration curve lower than 10%. The detection limit for tramadol in plasma was 0.2 ng mL-1. The proposed method was successfully applied to determination of tramadol in human plasma samples from 10 healthy volunteers after a single oral administration. © 2004 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/174928 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 0.584 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sha, YF | en_US |
dc.contributor.author | Shen, S | en_US |
dc.contributor.author | Duan, GL | en_US |
dc.date.accessioned | 2012-11-26T08:48:12Z | - |
dc.date.available | 2012-11-26T08:48:12Z | - |
dc.date.issued | 2005 | en_US |
dc.identifier.citation | Journal Of Pharmaceutical And Biomedical Analysis, 2005, v. 37 n. 1, p. 143-147 | en_US |
dc.identifier.issn | 0731-7085 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/174928 | - |
dc.description.abstract | A simple, rapid and sensitive method for determination of tramadol in plasma samples was developed using headspace solid-phase microextraction (HS-SPME) and gas chromatography with mass spectrometry (GC-MS). The optimum conditions for the SPME procedure were: headspace extraction on a 65-μm polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber; 0.5 mL of plasma modified with 0.5 mL of sodium hydroxide (0.1 M); extraction temperature of 100°C, with stirring at 2000 rpm for 30 min. The calibration curve showed linearity in the range of 1-400 ng mL-1 with regression coefficient corresponding to 0.9986 and coefficient of the variation of the points of the calibration curve lower than 10%. The detection limit for tramadol in plasma was 0.2 ng mL-1. The proposed method was successfully applied to determination of tramadol in human plasma samples from 10 healthy volunteers after a single oral administration. © 2004 Elsevier B.V. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpba | en_US |
dc.relation.ispartof | Journal of Pharmaceutical and Biomedical Analysis | en_US |
dc.subject | Gas chromatography-mass spectrometry | - |
dc.subject | Headspace solid-phase microextraction | - |
dc.subject | Human plasma | - |
dc.subject | Tramadol | - |
dc.subject.mesh | Electrophoresis, Capillary - Methods | en_US |
dc.subject.mesh | Gas Chromatography-Mass Spectrometry - Methods | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Time Factors | en_US |
dc.subject.mesh | Tramadol - Blood - Chemistry | en_US |
dc.title | Rapid determination of tramadol in human plasma by headspace solid-phase microextraction and capillary gas chromatography-mass spectrometry | en_US |
dc.type | Article | en_US |
dc.identifier.email | Shen, S: sshen@hkucc.hku.hk | en_US |
dc.identifier.authority | Shen, S=rp00775 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.jpba.2004.09.050 | en_US |
dc.identifier.pmid | 15664754 | - |
dc.identifier.scopus | eid_2-s2.0-12344335164 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-12344335164&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 37 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 143 | en_US |
dc.identifier.epage | 147 | en_US |
dc.identifier.isi | WOS:000227014200017 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Sha, YF=7102250309 | en_US |
dc.identifier.scopusauthorid | Shen, S=7403431266 | en_US |
dc.identifier.scopusauthorid | Duan, GL=7101615513 | en_US |
dc.identifier.issnl | 0731-7085 | - |