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postgraduate thesis: Transcriptional regulation of mouse secretin receptor in hypothalamic cells
| Title | Transcriptional regulation of mouse secretin receptor in hypothalamic cells |
|---|---|
| Authors | |
| Advisors | Advisor(s):Chow, BKC |
| Issue Date | 2011 |
| Publisher | The University of Hong Kong (Pokfulam, Hong Kong) |
| Citation | Yuan, Y. [袁媛]. (2011). Transcriptional regulation of mouse secretin receptor in hypothalamic cells. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775293 |
| Abstract |
As a neuropeptide, both secretin and secretin receptor are expressed in the central nervous system (CNS). It has been revealed that the activities of secretin on hypothalamic cells of rodents are important for osmoregulation and food intake. In the present study, embryonic mouse hypothalamic cell line N42 was used to study the promoter activity of mouse secretin receptor (mSR). By 5′ deletion analysis, a promoter element was identified within ?282 to ?443, relative to the ATG codon, and it contains a GC-box (-297 to -286), a ras responsive element (RRE) (-289 to -276) and an E-box (-416 to -411). Electrophoretic mobility shift assay (EMSA) and supershift analyses showed that Sp1 interacted with the GC-box, another zinc finger
As a neuropeptide, both secretin and secretin receptor are expressed in the central nervous system (CNS). It has been revealed that the activities of secretin on hypothalamic cells of rodents are important for osmoregulation and food intake. In the present study, embryonic mouse hypothalamic cell line N42 was used to study the promoter activity of mouse secretin receptor (mSR). By 5′ deletion analysis, a promoter element was identified within ?282 to ?443, relative to the ATG codon, and it contains a GC-box (-297 to -286), a ras responsive element (RRE) (-289 to -276) and an E-box (-416 to -411). Electrophoretic mobility shift assay (EMSA) and supershift analyses showed that Sp1 interacted with the GC-box, another zinc finger |
| Degree | Doctor of Philosophy |
| Subject | Secretin - Receptors. Transcription factors. Genetic transcription - Regulation. |
| Dept/Program | Biological Sciences |
| Persistent Identifier | http://hdl.handle.net/10722/174473 |
| HKU Library Item ID | b4775293 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Chow, BKC | - |
| dc.contributor.author | Yuan, Yuan | - |
| dc.contributor.author | 袁媛 | - |
| dc.date.issued | 2011 | - |
| dc.identifier.citation | Yuan, Y. [袁媛]. (2011). Transcriptional regulation of mouse secretin receptor in hypothalamic cells. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4775293 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/174473 | - |
| dc.description.abstract | As a neuropeptide, both secretin and secretin receptor are expressed in the central nervous system (CNS). It has been revealed that the activities of secretin on hypothalamic cells of rodents are important for osmoregulation and food intake. In the present study, embryonic mouse hypothalamic cell line N42 was used to study the promoter activity of mouse secretin receptor (mSR). By 5′ deletion analysis, a promoter element was identified within ?282 to ?443, relative to the ATG codon, and it contains a GC-box (-297 to -286), a ras responsive element (RRE) (-289 to -276) and an E-box (-416 to -411). Electrophoretic mobility shift assay (EMSA) and supershift analyses showed that Sp1 interacted with the GC-box, another zinc finger As a neuropeptide, both secretin and secretin receptor are expressed in the central nervous system (CNS). It has been revealed that the activities of secretin on hypothalamic cells of rodents are important for osmoregulation and food intake. In the present study, embryonic mouse hypothalamic cell line N42 was used to study the promoter activity of mouse secretin receptor (mSR). By 5′ deletion analysis, a promoter element was identified within ?282 to ?443, relative to the ATG codon, and it contains a GC-box (-297 to -286), a ras responsive element (RRE) (-289 to -276) and an E-box (-416 to -411). Electrophoretic mobility shift assay (EMSA) and supershift analyses showed that Sp1 interacted with the GC-box, another zinc finger | - |
| dc.language | eng | - |
| dc.publisher | The University of Hong Kong (Pokfulam, Hong Kong) | - |
| dc.relation.ispartof | HKU Theses Online (HKUTO) | - |
| dc.rights | The author retains all proprietary rights, (such as patent rights) and the right to use in future works. | - |
| dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
| dc.source.uri | http://hub.hku.hk/bib/B47752932 | - |
| dc.subject.lcsh | Secretin - Receptors. | - |
| dc.subject.lcsh | Transcription factors. | - |
| dc.subject.lcsh | Genetic transcription - Regulation. | - |
| dc.title | Transcriptional regulation of mouse secretin receptor in hypothalamic cells | - |
| dc.type | PG_Thesis | - |
| dc.identifier.hkul | b4775293 | - |
| dc.description.thesisname | Doctor of Philosophy | - |
| dc.description.thesislevel | Doctoral | - |
| dc.description.thesisdiscipline | Biological Sciences | - |
| dc.description.nature | published_or_final_version | - |
| dc.identifier.doi | 10.5353/th_b4775293 | - |
| dc.date.hkucongregation | 2012 | - |
| dc.identifier.mmsid | 991033466839703414 | - |