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postgraduate thesis: Evaluation of real time PCR assays and CHROMagar for laboratory diagnosis of methicillin resistant Staphylococcus aureus (MRSA)
Title | Evaluation of real time PCR assays and CHROMagar for laboratory diagnosis of methicillin resistant Staphylococcus aureus (MRSA) |
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Authors | |
Issue Date | 2012 |
Publisher | The University of Hong Kong (Pokfulam, Hong Kong) |
Citation | Fok, P. [霍碧君]. (2012). Evaluation of real time PCR assays and CHROMagar for laboratory diagnosis of methicillin resistant Staphylococcus aureus (MRSA). (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833381 |
Abstract | Methicillin-resistant Staphylococcus aureus (MRSA) is an important and common pathogen causing community- and healthcare-associated infection. Culture methods were used for identification of MRSA for a long period of time, however it spends a lot of time on incubation and 1 to 2 days is needed to obtain the identification and antibiogram. Molecular tests were developed in the past decades and different genes were used.
In this study a Staphylococcus aureus-specific gene, sau gene was designed and accompanied with mecA gene to detect the presence of MRSA in 322 nasal swabs from Tuen Mun Hospital. To evaluate the performance of in-house RT-PCR, samples were run in parallel with LightCycler? MRSA Advanced test and BBLTM CHROMagar? MRSA. 75 (23%) of samples were MRSA positive. The sensitivities and specificities of in-house RT-PCR and LightCycler? MRSA Advanced test were 76.7%/ 89.2% and 87.8%/ 96.6% respectively. The mean processing time for a batch of 32 samples by CHROMagar, in-house RT-PCR and LightCycler? MRSA Advanced test were 48.9 hours, 134.4 mins and 149.8 mins. In-house RT-PCR showed comparable performance and short turnaround time. sau gene can be used with mecA gene for the detection of MRSA in nasal swab. |
Degree | Master of Medical Sciences |
Subject | Staphylococcus aureus infections - Molecular diagnosis. Methicillin resistance. |
Dept/Program | Medicine |
Persistent Identifier | http://hdl.handle.net/10722/173947 |
HKU Library Item ID | b4833381 |
DC Field | Value | Language |
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dc.contributor.author | Fok, Pik-kwan. | - |
dc.contributor.author | 霍碧君. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Fok, P. [霍碧君]. (2012). Evaluation of real time PCR assays and CHROMagar for laboratory diagnosis of methicillin resistant Staphylococcus aureus (MRSA). (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833381 | - |
dc.identifier.uri | http://hdl.handle.net/10722/173947 | - |
dc.description.abstract | Methicillin-resistant Staphylococcus aureus (MRSA) is an important and common pathogen causing community- and healthcare-associated infection. Culture methods were used for identification of MRSA for a long period of time, however it spends a lot of time on incubation and 1 to 2 days is needed to obtain the identification and antibiogram. Molecular tests were developed in the past decades and different genes were used. In this study a Staphylococcus aureus-specific gene, sau gene was designed and accompanied with mecA gene to detect the presence of MRSA in 322 nasal swabs from Tuen Mun Hospital. To evaluate the performance of in-house RT-PCR, samples were run in parallel with LightCycler? MRSA Advanced test and BBLTM CHROMagar? MRSA. 75 (23%) of samples were MRSA positive. The sensitivities and specificities of in-house RT-PCR and LightCycler? MRSA Advanced test were 76.7%/ 89.2% and 87.8%/ 96.6% respectively. The mean processing time for a batch of 32 samples by CHROMagar, in-house RT-PCR and LightCycler? MRSA Advanced test were 48.9 hours, 134.4 mins and 149.8 mins. In-house RT-PCR showed comparable performance and short turnaround time. sau gene can be used with mecA gene for the detection of MRSA in nasal swab. | - |
dc.language | eng | - |
dc.publisher | The University of Hong Kong (Pokfulam, Hong Kong) | - |
dc.relation.ispartof | HKU Theses Online (HKUTO) | - |
dc.rights | The author retains all proprietary rights, (such as patent rights) and the right to use in future works. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.source.uri | http://hub.hku.hk/bib/B48333815 | - |
dc.subject.lcsh | Staphylococcus aureus infections - Molecular diagnosis. | - |
dc.subject.lcsh | Methicillin resistance. | - |
dc.title | Evaluation of real time PCR assays and CHROMagar for laboratory diagnosis of methicillin resistant Staphylococcus aureus (MRSA) | - |
dc.type | PG_Thesis | - |
dc.identifier.hkul | b4833381 | - |
dc.description.thesisname | Master of Medical Sciences | - |
dc.description.thesislevel | Master | - |
dc.description.thesisdiscipline | Medicine | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.5353/th_b4833381 | - |
dc.date.hkucongregation | 2012 | - |
dc.identifier.mmsid | 991033833449703414 | - |