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Conference Paper: Sequence comparison of the EcoHK31 I and EaeI restriction-modification systems suggests an intergenic transfer of genetic material

TitleSequence comparison of the EcoHK31 I and EaeI restriction-modification systems suggests an intergenic transfer of genetic material
Authors
Issue Date1998
PublisherWalter de Gruyter GmbH & Co KG. The Journal's web site is located at http://www.degruyter.de/journals/bc
Citation
Biological Chemistry, 1998, v. 379 n. 4-5, p. 437-441 How to Cite?
AbstractThe genes coding for the EcoHK31I and EaeI restriction-modification (R-M) systems from Escherichia coli strain HK31 and Enterobacter aerogenes, respectively, have been cloned and sequenced. Both ENases recognize and cleave Y↓GGCCR leaving 4 nucleotide 5'-protruding ends, while the MTases modify the internal cytosine. The systems were isolated on a 2.3 kb AseI fragment for EcoHK31I, and a 4.6 kb HindIII fragment for EaeI. The R and M genes of both systems converge and overlap by 14 nucleotides. Previously, we found that M.EcoHK31I consisted of two subunits, (α and β), with the β subunit being translated from an alternative open reading frame within the gene encoding the or subunit. Sequence comparison between the EcoHK31I and EaeI systems reveals striking similarity. The eaeIM gene also encodes α and β polypeptides of 309 and 176 amino acids which share 96% and 97% identity, respectively, with those of ecoHK31IM, ecoHK31IR and eaeIR encode proteins of 318 and 315 aa, respectively, which share 92% identity but are otherwise unique in the GenBank database. The EaeI and the EcoHK31I R-M systems were found to be flanked by genes coding for integrases. It is possible that these integrases have facilitated the transfer of this system among different bacterial species.
Persistent Identifierhttp://hdl.handle.net/10722/173566
ISSN
2015 Impact Factor: 2.71
2015 SCImago Journal Rankings: 1.607
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, KFen_US
dc.contributor.authorShaw, PCen_US
dc.contributor.authorPicone, SJen_US
dc.contributor.authorWilson, GGen_US
dc.contributor.authorLunnen, KDen_US
dc.date.accessioned2012-10-30T06:33:24Z-
dc.date.available2012-10-30T06:33:24Z-
dc.date.issued1998en_US
dc.identifier.citationBiological Chemistry, 1998, v. 379 n. 4-5, p. 437-441en_US
dc.identifier.issn1431-6730en_US
dc.identifier.urihttp://hdl.handle.net/10722/173566-
dc.description.abstractThe genes coding for the EcoHK31I and EaeI restriction-modification (R-M) systems from Escherichia coli strain HK31 and Enterobacter aerogenes, respectively, have been cloned and sequenced. Both ENases recognize and cleave Y↓GGCCR leaving 4 nucleotide 5'-protruding ends, while the MTases modify the internal cytosine. The systems were isolated on a 2.3 kb AseI fragment for EcoHK31I, and a 4.6 kb HindIII fragment for EaeI. The R and M genes of both systems converge and overlap by 14 nucleotides. Previously, we found that M.EcoHK31I consisted of two subunits, (α and β), with the β subunit being translated from an alternative open reading frame within the gene encoding the or subunit. Sequence comparison between the EcoHK31I and EaeI systems reveals striking similarity. The eaeIM gene also encodes α and β polypeptides of 309 and 176 amino acids which share 96% and 97% identity, respectively, with those of ecoHK31IM, ecoHK31IR and eaeIR encode proteins of 318 and 315 aa, respectively, which share 92% identity but are otherwise unique in the GenBank database. The EaeI and the EcoHK31I R-M systems were found to be flanked by genes coding for integrases. It is possible that these integrases have facilitated the transfer of this system among different bacterial species.en_US
dc.languageengen_US
dc.publisherWalter de Gruyter GmbH & Co KG. The Journal's web site is located at http://www.degruyter.de/journals/bcen_US
dc.relation.ispartofBiological Chemistryen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshConjugation, Geneticen_US
dc.subject.meshDna, Bacterialen_US
dc.subject.meshDeoxyribonucleases, Type Ii Site-Specific - Geneticsen_US
dc.subject.meshEnterobacter - Geneticsen_US
dc.subject.meshEscherichia Coli - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshRecombination, Geneticen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.titleSequence comparison of the EcoHK31 I and EaeI restriction-modification systems suggests an intergenic transfer of genetic materialen_US
dc.typeConference_Paperen_US
dc.identifier.emailLee, KF:ckflee@hku.hken_US
dc.identifier.authorityLee, KF=rp00458en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1515/bchm.1998.379.4-5.437-
dc.identifier.pmid9628335en_US
dc.identifier.scopuseid_2-s2.0-0031596401en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031596401&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume379en_US
dc.identifier.issue4-5en_US
dc.identifier.spage437en_US
dc.identifier.epage441en_US
dc.identifier.isiWOS:000073686200010-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridLee, KF=26643097500en_US
dc.identifier.scopusauthoridShaw, PC=35599523600en_US
dc.identifier.scopusauthoridPicone, SJ=7801638815en_US
dc.identifier.scopusauthoridWilson, GG=7404529877en_US
dc.identifier.scopusauthoridLunnen, KD=6603092080en_US

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