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Conference Paper: H Permeability of apical membrane vesicles from the upper gastrointestinal tract

TitleH Permeability of apical membrane vesicles from the upper gastrointestinal tract
Authors
Issue Date1988
Citation
Comparative Biochemistry and Physiology -- Part A: Physiology, 1988, v. 90 n. 4, p. 840, abstract no. G11 How to Cite?
AbstractApical membrane vesicles were isolated from the rabbit brush border (dBBMV) and histamine stimulated oxyntic cells (SAV). Proton permeation was monitored from acridine orange fluorescence quenching,+following imposition of a transmembrane H gradient. The process followed first order kinetics, with rate constants o 4. 520.22 (n=20) and 2.15k0.14 (n= 14) x lo-?s-f (temp=20°C). for dBBMV and SAV respectively Fluorescence anisotropy (r) of diphenylhexatriene indicated the fluidity of the lipid bilayer (expressed as (r Me/ r)-1, r = limiting anisotropy (0.362)). dBB and &V gave values of 0.488+0.013 (n=7) and 0.66* (n==4) respectively. Increases in temperature, and treatment with a sqries of alcohols, increased(rdr)-1, and H permeation in the case of SAV than dBBMV. Arrhenius plots of the two parameters demonstrated discontinuities at 30°C in the case of dBBMV, but not SAV.
Persistent Identifierhttp://hdl.handle.net/10722/173448
ISSN
2000 SCImago Journal Rankings: 0.403

 

DC FieldValueLanguage
dc.contributor.authorWilkes, JMen_US
dc.contributor.authorBallard, HJen_US
dc.contributor.authorHirst, BHen_US
dc.date.accessioned2012-10-30T06:31:29Z-
dc.date.available2012-10-30T06:31:29Z-
dc.date.issued1988en_US
dc.identifier.citationComparative Biochemistry and Physiology -- Part A: Physiology, 1988, v. 90 n. 4, p. 840, abstract no. G11en_US
dc.identifier.issn0300-9629en_US
dc.identifier.urihttp://hdl.handle.net/10722/173448-
dc.description.abstractApical membrane vesicles were isolated from the rabbit brush border (dBBMV) and histamine stimulated oxyntic cells (SAV). Proton permeation was monitored from acridine orange fluorescence quenching,+following imposition of a transmembrane H gradient. The process followed first order kinetics, with rate constants o 4. 520.22 (n=20) and 2.15k0.14 (n= 14) x lo-?s-f (temp=20°C). for dBBMV and SAV respectively Fluorescence anisotropy (r) of diphenylhexatriene indicated the fluidity of the lipid bilayer (expressed as (r Me/ r)-1, r = limiting anisotropy (0.362)). dBB and &V gave values of 0.488+0.013 (n=7) and 0.66* (n==4) respectively. Increases in temperature, and treatment with a sqries of alcohols, increased(rdr)-1, and H permeation in the case of SAV than dBBMV. Arrhenius plots of the two parameters demonstrated discontinuities at 30°C in the case of dBBMV, but not SAV.-
dc.languageengen_US
dc.relation.ispartofComparative Biochemistry and Physiology -- Part A: Physiologyen_US
dc.titleH Permeability of apical membrane vesicles from the upper gastrointestinal tracten_US
dc.typeConference_Paperen_US
dc.identifier.emailBallard, HJ:ballard@hkucc.hku.hken_US
dc.identifier.authorityBallard, HJ=rp00367en_US
dc.description.natureLink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-45549111166en_US
dc.identifier.volume90en_US
dc.identifier.issue4en_US
dc.identifier.spage840, abstract no. G11en_US
dc.identifier.epage840, abstract no. G11-
dc.identifier.scopusauthoridWilkes, JM=7102513233en_US
dc.identifier.scopusauthoridBallard, HJ=7005286310en_US
dc.identifier.scopusauthoridHirst, BH=7005256376en_US
dc.customcontrol.immutablesml 170213 amended-

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