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Conference Paper: Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering
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TitleSelective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering
 
AuthorsDuan, B1
Lu, WW1
Wang, M1
 
Issue Date2011
 
PublisherAmerican Ceramic Society Inc. The Journal's web site is located at http://www.ceramics.org/publications/cesp.asp
 
CitationThe 35th International Conference and Exposition on Advanced Ceramics and Composites (ICACC' 2011), Daytona Beach Florida, USA, 23-28 January 2011. In Ceramic Engineering and Science Proceedings, 2011, v. 32 n. 6, p. 37-48 [How to Cite?]
 
AbstractCa-P/PHBV nanocomposite scaffolds for bone tissue engineering were fabricated via selective laser sintering. The surface modification of Ca-P/PHBV scaffolds was conducted firstly by physical entrapment of gelatin. Heparin was then immobilized on gelatin-modified scaffolds through covalent conjugation. Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) were seeded onto the scaffolds. Compared to non-modified scaffolds, heparin-immobilized scaffolds exhibited higher cell proliferation at the early stage of cell culture. hUC-MSCs became confluent after 21 day culture on scaffolds and covered the whole scaffold surface, strongly adhering to the scaffolds. Recombinant human bone morphogenetic protein (rhBMP)-2 was loaded onto scaffolds with or without surface modification and its in vitro release behavior was studied. An initial burst release of rhBMP-2 was observed for both types of scaffolds. However, the immobilization of heparin on the surface of Ca-P/PHBV scaffolds not only provided a means to protect the rhBMP-2 but also improved its sustained release. Surface modified scaffolds loaded with rhBMP-2 promoted significantly higher ALP activity of hUC-MSCs than the scaffolds with simple adsorption of rhBMP-2. The strategy of combining advanced scaffold fabrication technology, nanocomposite and growth factor delivery is promising for bone tissue regeneration.
 
ISSN0196-6219
2013 SCImago Journal Rankings: 0.190
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorDuan, B
 
dc.contributor.authorLu, WW
 
dc.contributor.authorWang, M
 
dc.date.accessioned2012-10-30T06:31:02Z
 
dc.date.available2012-10-30T06:31:02Z
 
dc.date.issued2011
 
dc.description.abstractCa-P/PHBV nanocomposite scaffolds for bone tissue engineering were fabricated via selective laser sintering. The surface modification of Ca-P/PHBV scaffolds was conducted firstly by physical entrapment of gelatin. Heparin was then immobilized on gelatin-modified scaffolds through covalent conjugation. Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) were seeded onto the scaffolds. Compared to non-modified scaffolds, heparin-immobilized scaffolds exhibited higher cell proliferation at the early stage of cell culture. hUC-MSCs became confluent after 21 day culture on scaffolds and covered the whole scaffold surface, strongly adhering to the scaffolds. Recombinant human bone morphogenetic protein (rhBMP)-2 was loaded onto scaffolds with or without surface modification and its in vitro release behavior was studied. An initial burst release of rhBMP-2 was observed for both types of scaffolds. However, the immobilization of heparin on the surface of Ca-P/PHBV scaffolds not only provided a means to protect the rhBMP-2 but also improved its sustained release. Surface modified scaffolds loaded with rhBMP-2 promoted significantly higher ALP activity of hUC-MSCs than the scaffolds with simple adsorption of rhBMP-2. The strategy of combining advanced scaffold fabrication technology, nanocomposite and growth factor delivery is promising for bone tissue regeneration.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationThe 35th International Conference and Exposition on Advanced Ceramics and Composites (ICACC' 2011), Daytona Beach Florida, USA, 23-28 January 2011. In Ceramic Engineering and Science Proceedings, 2011, v. 32 n. 6, p. 37-48 [How to Cite?]
 
dc.identifier.epage48
 
dc.identifier.hkuros194125
 
dc.identifier.issn0196-6219
2013 SCImago Journal Rankings: 0.190
 
dc.identifier.issue6
 
dc.identifier.scopuseid_2-s2.0-82355175439
 
dc.identifier.spage37
 
dc.identifier.urihttp://hdl.handle.net/10722/173423
 
dc.identifier.volume32
 
dc.languageeng
 
dc.publisherAmerican Ceramic Society Inc. The Journal's web site is located at http://www.ceramics.org/publications/cesp.asp
 
dc.publisher.placeUnited States
 
dc.relation.ispartofCeramic Engineering and Science Proceedings
 
dc.relation.referencesReferences in Scopus
 
dc.titleSelective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering
 
dc.typeConference_Paper
 
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Author Affiliations
  1. The University of Hong Kong