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Conference Paper: Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering

TitleSelective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering
Authors
Issue Date2011
PublisherAmerican Ceramic Society Inc. The Journal's web site is located at http://www.ceramics.org/publications/cesp.asp
Citation
The 35th International Conference and Exposition on Advanced Ceramics and Composites (ICACC' 2011), Daytona Beach Florida, USA, 23-28 January 2011. In Ceramic Engineering and Science Proceedings, 2011, v. 32 n. 6, p. 37-48 How to Cite?
AbstractCa-P/PHBV nanocomposite scaffolds for bone tissue engineering were fabricated via selective laser sintering. The surface modification of Ca-P/PHBV scaffolds was conducted firstly by physical entrapment of gelatin. Heparin was then immobilized on gelatin-modified scaffolds through covalent conjugation. Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) were seeded onto the scaffolds. Compared to non-modified scaffolds, heparin-immobilized scaffolds exhibited higher cell proliferation at the early stage of cell culture. hUC-MSCs became confluent after 21 day culture on scaffolds and covered the whole scaffold surface, strongly adhering to the scaffolds. Recombinant human bone morphogenetic protein (rhBMP)-2 was loaded onto scaffolds with or without surface modification and its in vitro release behavior was studied. An initial burst release of rhBMP-2 was observed for both types of scaffolds. However, the immobilization of heparin on the surface of Ca-P/PHBV scaffolds not only provided a means to protect the rhBMP-2 but also improved its sustained release. Surface modified scaffolds loaded with rhBMP-2 promoted significantly higher ALP activity of hUC-MSCs than the scaffolds with simple adsorption of rhBMP-2. The strategy of combining advanced scaffold fabrication technology, nanocomposite and growth factor delivery is promising for bone tissue regeneration.
Persistent Identifierhttp://hdl.handle.net/10722/173423
ISSN
2013 SCImago Journal Rankings: 0.190
References

 

DC FieldValueLanguage
dc.contributor.authorDuan, Ben_US
dc.contributor.authorLu, WWen_US
dc.contributor.authorWang, Men_US
dc.date.accessioned2012-10-30T06:31:02Z-
dc.date.available2012-10-30T06:31:02Z-
dc.date.issued2011en_US
dc.identifier.citationThe 35th International Conference and Exposition on Advanced Ceramics and Composites (ICACC' 2011), Daytona Beach Florida, USA, 23-28 January 2011. In Ceramic Engineering and Science Proceedings, 2011, v. 32 n. 6, p. 37-48en_US
dc.identifier.issn0196-6219en_US
dc.identifier.urihttp://hdl.handle.net/10722/173423-
dc.description.abstractCa-P/PHBV nanocomposite scaffolds for bone tissue engineering were fabricated via selective laser sintering. The surface modification of Ca-P/PHBV scaffolds was conducted firstly by physical entrapment of gelatin. Heparin was then immobilized on gelatin-modified scaffolds through covalent conjugation. Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) were seeded onto the scaffolds. Compared to non-modified scaffolds, heparin-immobilized scaffolds exhibited higher cell proliferation at the early stage of cell culture. hUC-MSCs became confluent after 21 day culture on scaffolds and covered the whole scaffold surface, strongly adhering to the scaffolds. Recombinant human bone morphogenetic protein (rhBMP)-2 was loaded onto scaffolds with or without surface modification and its in vitro release behavior was studied. An initial burst release of rhBMP-2 was observed for both types of scaffolds. However, the immobilization of heparin on the surface of Ca-P/PHBV scaffolds not only provided a means to protect the rhBMP-2 but also improved its sustained release. Surface modified scaffolds loaded with rhBMP-2 promoted significantly higher ALP activity of hUC-MSCs than the scaffolds with simple adsorption of rhBMP-2. The strategy of combining advanced scaffold fabrication technology, nanocomposite and growth factor delivery is promising for bone tissue regeneration.en_US
dc.languageengen_US
dc.publisherAmerican Ceramic Society Inc. The Journal's web site is located at http://www.ceramics.org/publications/cesp.aspen_US
dc.relation.ispartofCeramic Engineering and Science Proceedingsen_US
dc.titleSelective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineeringen_US
dc.typeConference_Paperen_US
dc.identifier.emailLu, WW:wwlu@hku.hken_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-82355175439en_US
dc.identifier.hkuros194125-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-82355175439&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume32en_US
dc.identifier.issue6en_US
dc.identifier.spage37en_US
dc.identifier.epage48en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridDuan, B=7005042335en_US
dc.identifier.scopusauthoridLu, WW=7404215221en_US
dc.identifier.scopusauthoridWang, M=36077223400en_US

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