Conference Paper: Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering
| Title | Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering |
|---|---|
| Authors | Duan, B1 Lu, WW1 Wang, M1 |
| Issue Date | 2011 |
| Publisher | American Ceramic Society Inc. The Journal's web site is located at http://www.ceramics.org/publications/cesp.asp |
| Citation | Ceramic Engineering And Science Proceedings, 2011, v. 32 n. 6, p. 37-48 [How to Cite?] |
| Abstract | Ca-P/PHBV nanocomposite scaffolds for bone tissue engineering were fabricated via selective laser sintering. The surface modification of Ca-P/PHBV scaffolds was conducted firstly by physical entrapment of gelatin. Heparin was then immobilized on gelatin-modified scaffolds through covalent conjugation. Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) were seeded onto the scaffolds. Compared to non-modified scaffolds, heparin-immobilized scaffolds exhibited higher cell proliferation at the early stage of cell culture. hUC-MSCs became confluent after 21 day culture on scaffolds and covered the whole scaffold surface, strongly adhering to the scaffolds. Recombinant human bone morphogenetic protein (rhBMP)-2 was loaded onto scaffolds with or without surface modification and its in vitro release behavior was studied. An initial burst release of rhBMP-2 was observed for both types of scaffolds. However, the immobilization of heparin on the surface of Ca-P/PHBV scaffolds not only provided a means to protect the rhBMP-2 but also improved its sustained release. Surface modified scaffolds loaded with rhBMP-2 promoted significantly higher ALP activity of hUC-MSCs than the scaffolds with simple adsorption of rhBMP-2. The strategy of combining advanced scaffold fabrication technology, nanocomposite and growth factor delivery is promising for bone tissue regeneration. |
| ISSN | 0196-6219 2011 SCImago Journal Rankings: 0.027 |
| References | References in Scopus |
| dc.contributor.author | Duan, B |
|---|---|
| dc.contributor.author | Lu, WW |
| dc.contributor.author | Wang, M |
| dc.date.accessioned | 2012-10-30T06:31:02Z |
| dc.date.available | 2012-10-30T06:31:02Z |
| dc.date.issued | 2011 |
| dc.description.abstract | Ca-P/PHBV nanocomposite scaffolds for bone tissue engineering were fabricated via selective laser sintering. The surface modification of Ca-P/PHBV scaffolds was conducted firstly by physical entrapment of gelatin. Heparin was then immobilized on gelatin-modified scaffolds through covalent conjugation. Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) were seeded onto the scaffolds. Compared to non-modified scaffolds, heparin-immobilized scaffolds exhibited higher cell proliferation at the early stage of cell culture. hUC-MSCs became confluent after 21 day culture on scaffolds and covered the whole scaffold surface, strongly adhering to the scaffolds. Recombinant human bone morphogenetic protein (rhBMP)-2 was loaded onto scaffolds with or without surface modification and its in vitro release behavior was studied. An initial burst release of rhBMP-2 was observed for both types of scaffolds. However, the immobilization of heparin on the surface of Ca-P/PHBV scaffolds not only provided a means to protect the rhBMP-2 but also improved its sustained release. Surface modified scaffolds loaded with rhBMP-2 promoted significantly higher ALP activity of hUC-MSCs than the scaffolds with simple adsorption of rhBMP-2. The strategy of combining advanced scaffold fabrication technology, nanocomposite and growth factor delivery is promising for bone tissue regeneration. |
| dc.description.nature | Link_to_subscribed_fulltext |
| dc.identifier.citation | Ceramic Engineering And Science Proceedings, 2011, v. 32 n. 6, p. 37-48 [How to Cite?] |
| dc.identifier.epage | 48 |
| dc.identifier.issn | 0196-6219 2011 SCImago Journal Rankings: 0.027 |
| dc.identifier.issue | 6 |
| dc.identifier.scopus | eid_2-s2.0-82355175439 |
| dc.identifier.spage | 37 |
| dc.identifier.uri | http://hdl.handle.net/10722/173423 |
| dc.identifier.volume | 32 |
| dc.language | eng |
| dc.publisher | American Ceramic Society Inc. The Journal's web site is located at http://www.ceramics.org/publications/cesp.asp |
| dc.publisher.place | United States |
| dc.relation.ispartof | Ceramic Engineering and Science Proceedings |
| dc.relation.references | References in Scopus |
| dc.title | Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering |
| dc.type | Conference_Paper |
Author Affiliations
- The University of Hong Kong