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Article: Differential Growth, Cell Proliferation, and Apoptosis of Mouse Embryo in Various Culture Media and in Coculture

TitleDifferential Growth, Cell Proliferation, and Apoptosis of Mouse Embryo in Various Culture Media and in Coculture
Authors
KeywordsApoptosis
Embryo
Oviduct
Proliferation
Issue Date2004
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692
Citation
Molecular Reproduction And Development, 2004, v. 68 n. 1, p. 72-80 How to Cite?
AbstractSequential culture and coculture are two methods of improving the development of preimplantation embryos in vitro. Direct comparison of the efficiency of these methods is limited. Proliferation and apoptosis determine the total number of blastomere in preimplantation embryo, which is a sensitive parameter for evaluation of the development of embryo in vitro. In this study, we compared the proliferation and apoptosis of mouse embryo in different culture media, including CZB, KSOM, MTF, G1.2/G2.2 sequential culture media, and in human oviductal cell coculture. Sequential culture using G1.2/G2.2 was superior to KSOM, MTF, and CZB/CZB + G with respect to the formation of 3-4 cell embryos, morula, and blastocyst. G1.2/G2.2 cultured blastocyst had significantly more proliferating blastomeres and higher total cell number per blastocyst than those cultured in KSOM or CZB/CZB + G. Compared to embryos cultured in G1.2/G2.2, embryos cocultured in G1.2/G2.2 hatched more frequently. Cocultured blastocysts also had significantly higher percentage of proliferating cell and lower percentage of apoptotic cell per blastocyst than those cultured in G1.2/G2.2. It was concluded that G1.2/G2.2 facilitated the proliferation of blastomere whilst human oviductal cell coculture suppressed apoptosis in addition to stimulating proliferation of blastomere. © 2004 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/173282
ISSN
2015 Impact Factor: 2.141
2015 SCImago Journal Rankings: 1.041
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, JSen_HK
dc.contributor.authorChan, STHen_HK
dc.contributor.authorLee, WWMen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2012-10-30T06:29:04Z-
dc.date.available2012-10-30T06:29:04Z-
dc.date.issued2004en_HK
dc.identifier.citationMolecular Reproduction And Development, 2004, v. 68 n. 1, p. 72-80en_HK
dc.identifier.issn1040-452Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/173282-
dc.description.abstractSequential culture and coculture are two methods of improving the development of preimplantation embryos in vitro. Direct comparison of the efficiency of these methods is limited. Proliferation and apoptosis determine the total number of blastomere in preimplantation embryo, which is a sensitive parameter for evaluation of the development of embryo in vitro. In this study, we compared the proliferation and apoptosis of mouse embryo in different culture media, including CZB, KSOM, MTF, G1.2/G2.2 sequential culture media, and in human oviductal cell coculture. Sequential culture using G1.2/G2.2 was superior to KSOM, MTF, and CZB/CZB + G with respect to the formation of 3-4 cell embryos, morula, and blastocyst. G1.2/G2.2 cultured blastocyst had significantly more proliferating blastomeres and higher total cell number per blastocyst than those cultured in KSOM or CZB/CZB + G. Compared to embryos cultured in G1.2/G2.2, embryos cocultured in G1.2/G2.2 hatched more frequently. Cocultured blastocysts also had significantly higher percentage of proliferating cell and lower percentage of apoptotic cell per blastocyst than those cultured in G1.2/G2.2. It was concluded that G1.2/G2.2 facilitated the proliferation of blastomere whilst human oviductal cell coculture suppressed apoptosis in addition to stimulating proliferation of blastomere. © 2004 Wiley-Liss, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692en_HK
dc.relation.ispartofMolecular Reproduction and Developmenten_HK
dc.rightsMolecular Reproduction and Development. Copyright © John Wiley & Sons, Inc.-
dc.subjectApoptosisen_HK
dc.subjectEmbryoen_HK
dc.subjectOviducten_HK
dc.subjectProliferationen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshBlastocyst - Cytology - Drug Effectsen_US
dc.subject.meshBlastomeres - Cytology - Drug Effectsen_US
dc.subject.meshCell Division - Drug Effectsen_US
dc.subject.meshCell Sizeen_US
dc.subject.meshCoculture Techniquesen_US
dc.subject.meshCulture Media - Pharmacologyen_US
dc.subject.meshEmbryo, Mammalian - Cytology - Drug Effects - Embryologyen_US
dc.subject.meshEmbryonic And Fetal Development - Drug Effectsen_US
dc.subject.meshFallopian Tubes - Cytologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMorula - Cytology - Drug Effectsen_US
dc.titleDifferential Growth, Cell Proliferation, and Apoptosis of Mouse Embryo in Various Culture Media and in Cocultureen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, WWM: hrszlwm@hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, WWM=rp00728en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/mrd.20048en_HK
dc.identifier.pmid15039950-
dc.identifier.scopuseid_2-s2.0-1842480432en_HK
dc.identifier.hkuros88140-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1842480432&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume68en_HK
dc.identifier.issue1en_HK
dc.identifier.spage72en_HK
dc.identifier.epage80en_HK
dc.identifier.isiWOS:000220582400009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXu, JS=7408556691en_HK
dc.identifier.scopusauthoridChan, STH=24368283200en_HK
dc.identifier.scopusauthoridLee, WWM=24799156600en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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