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Article: Determination of the Binding Constant Between Bovine Serum Albumin and Its Monoclonal Antibody by Affinity Capillary Electrophoresis with Laser Induced Fluorescence Detection

TitleDetermination of the Binding Constant Between Bovine Serum Albumin and Its Monoclonal Antibody by Affinity Capillary Electrophoresis with Laser Induced Fluorescence Detection
Authors
KeywordsAffinity Capillary Electrophoresis
Binding Constant
Laser Induced Fluorescence Detection
Protein
Issue Date1999
PublisherJilin Daxue. The Journal's web site is located at http://www.cjcu.jlu.edu.cn/
Citation
Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal Of Chinese Universities, 1999, v. 20 n. 10, p. 1553 How to Cite?
AbstractThe binding constant (Kb) between fluorescein isothiocyanate labeled bovine serum albumin (FITC-BSA) and its monoclonal antibody was determined using the ligand separation mode of affinity capillary electrophoresis (ACE) with laser induced fluorescence detection (LIF). Free FITC-BSA and the complexes were separated by capillary electrophoresis in 7 min after incubation. The running buffer was 100 mmol/L HEPES at pH 7. 4, and separation voltage was 30 kV. The Kb was determined to be 4.7 × 108 L/mol using Scatchard plotting method. The influences of incubation time and electrophoresis conditions on determination were also studied.
Persistent Identifierhttp://hdl.handle.net/10722/173272
ISSN
2015 Impact Factor: 0.791
2015 SCImago Journal Rankings: 0.211
References

 

DC FieldValueLanguage
dc.contributor.authorWang, QGen_US
dc.contributor.authorLuo, GAen_US
dc.contributor.authorYeung, WSBen_US
dc.date.accessioned2012-10-30T06:28:58Z-
dc.date.available2012-10-30T06:28:58Z-
dc.date.issued1999en_US
dc.identifier.citationKao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal Of Chinese Universities, 1999, v. 20 n. 10, p. 1553en_US
dc.identifier.issn0251-0790en_US
dc.identifier.urihttp://hdl.handle.net/10722/173272-
dc.description.abstractThe binding constant (Kb) between fluorescein isothiocyanate labeled bovine serum albumin (FITC-BSA) and its monoclonal antibody was determined using the ligand separation mode of affinity capillary electrophoresis (ACE) with laser induced fluorescence detection (LIF). Free FITC-BSA and the complexes were separated by capillary electrophoresis in 7 min after incubation. The running buffer was 100 mmol/L HEPES at pH 7. 4, and separation voltage was 30 kV. The Kb was determined to be 4.7 × 108 L/mol using Scatchard plotting method. The influences of incubation time and electrophoresis conditions on determination were also studied.en_US
dc.languageengen_US
dc.publisherJilin Daxue. The Journal's web site is located at http://www.cjcu.jlu.edu.cn/en_US
dc.relation.ispartofKao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universitiesen_US
dc.subjectAffinity Capillary Electrophoresisen_US
dc.subjectBinding Constanten_US
dc.subjectLaser Induced Fluorescence Detectionen_US
dc.subjectProteinen_US
dc.titleDetermination of the Binding Constant Between Bovine Serum Albumin and Its Monoclonal Antibody by Affinity Capillary Electrophoresis with Laser Induced Fluorescence Detectionen_US
dc.typeArticleen_US
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0345880851en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0345880851&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume20en_US
dc.identifier.issue10en_US
dc.identifier.spage1553en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridWang, QG=7406915351en_US
dc.identifier.scopusauthoridLuo, GA=7401536462en_US
dc.identifier.scopusauthoridYeung, WSB=7102370745en_US

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