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Article: Cloning and characterization of the human oviduct-specific gycoprotein (HuOGP) gene promoter

TitleCloning and characterization of the human oviduct-specific gycoprotein (HuOGP) gene promoter
Authors
Issue Date2002
PublisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/
Citation
Molecular Human Reproduction, 2002, v. 8 n. 2, p. 167-175 How to Cite?
AbstractOviduct-specific glycoprotein (OGP) is a high molecular weight glycoprotein belonging to the chitinase protein family. OGPs are found to associate with the zona pellucida and plasma membrane of the oocyte and developing embryo. Recent studies have shown that OGP plays an important role in pre-fertilization reproductive events (i.e. sperm capacitation, sperm-zona binding and zona penetration). To have a better understanding of human OGP (HuOGP) gene expression, a 3.3 kb DNA fragment containing the 5′-flanking and the intron I region of the HuOGP gene was isolated. DNA sequence analysis of the HuOGP putative promoter revealed little homology with its hamster and mouse ogp gene counterparts. One transcription initiation site was found 12 nucleotides upstream of the first ATG codon of the HuOGP gene. The HuOGP gene promoter lacked typical CAAT or GC boxes, but contained eight half estrogen-responsive elements (ERE) and an imperfect ERE (iERE; 5′-GGTCANNNTGACT-3′) site. Deletion mutants of the 3.3 kb DNA fragment were generated and fused to a promoterless β-galactosidase (β-Gal) gene. Transfection studies revealed that in the presence of 100 nmol/l estradiol-17β (E2), a minimal 0.3 kb promoter construct (pH-298/+25βGal) mediated a high level of β-Gal expression in immortalized human oviductal epithelial OE-E6/E7 cells, but not in MCF-7 and CHO-K1 cells. By electromobility shift assay and specific estrogen receptor antibodies, we demonstrated that estrogen receptor β present in the OE-E6/E7 cells binds to the iERE. These findings allow a better understanding of the regulation of OGP gene expression in the human oviduct.
Persistent Identifierhttp://hdl.handle.net/10722/173260
ISSN
2015 Impact Factor: 3.943
2015 SCImago Journal Rankings: 1.611
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAgarwal, Aen_US
dc.contributor.authorYeung, WSBen_US
dc.contributor.authorLee, KFen_US
dc.date.accessioned2012-10-30T06:28:52Z-
dc.date.available2012-10-30T06:28:52Z-
dc.date.issued2002en_US
dc.identifier.citationMolecular Human Reproduction, 2002, v. 8 n. 2, p. 167-175en_US
dc.identifier.issn1360-9947en_US
dc.identifier.urihttp://hdl.handle.net/10722/173260-
dc.description.abstractOviduct-specific glycoprotein (OGP) is a high molecular weight glycoprotein belonging to the chitinase protein family. OGPs are found to associate with the zona pellucida and plasma membrane of the oocyte and developing embryo. Recent studies have shown that OGP plays an important role in pre-fertilization reproductive events (i.e. sperm capacitation, sperm-zona binding and zona penetration). To have a better understanding of human OGP (HuOGP) gene expression, a 3.3 kb DNA fragment containing the 5′-flanking and the intron I region of the HuOGP gene was isolated. DNA sequence analysis of the HuOGP putative promoter revealed little homology with its hamster and mouse ogp gene counterparts. One transcription initiation site was found 12 nucleotides upstream of the first ATG codon of the HuOGP gene. The HuOGP gene promoter lacked typical CAAT or GC boxes, but contained eight half estrogen-responsive elements (ERE) and an imperfect ERE (iERE; 5′-GGTCANNNTGACT-3′) site. Deletion mutants of the 3.3 kb DNA fragment were generated and fused to a promoterless β-galactosidase (β-Gal) gene. Transfection studies revealed that in the presence of 100 nmol/l estradiol-17β (E2), a minimal 0.3 kb promoter construct (pH-298/+25βGal) mediated a high level of β-Gal expression in immortalized human oviductal epithelial OE-E6/E7 cells, but not in MCF-7 and CHO-K1 cells. By electromobility shift assay and specific estrogen receptor antibodies, we demonstrated that estrogen receptor β present in the OE-E6/E7 cells binds to the iERE. These findings allow a better understanding of the regulation of OGP gene expression in the human oviduct.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/en_US
dc.relation.ispartofMolecular Human Reproductionen_US
dc.rightsMolecular Human Reproduction. Copyright © Oxford University Press.-
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCho Cellsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshDnaen_US
dc.subject.meshEstrogens - Physiologyen_US
dc.subject.meshFallopian Tubes - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshGlycoproteins - Chemistry - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.titleCloning and characterization of the human oviduct-specific gycoprotein (HuOGP) gene promoteren_US
dc.typeArticleen_US
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_US
dc.identifier.emailLee, KF:ckflee@hku.hken_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.identifier.authorityLee, KF=rp00458en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/molehr/8.2.167-
dc.identifier.pmid11818519-
dc.identifier.scopuseid_2-s2.0-0036171203en_US
dc.identifier.hkuros65449-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036171203&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume8en_US
dc.identifier.issue2en_US
dc.identifier.spage167en_US
dc.identifier.epage175en_US
dc.identifier.isiWOS:000173754400008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridAgarwal, A=36922115000en_US
dc.identifier.scopusauthoridYeung, WSB=7102370745en_US
dc.identifier.scopusauthoridLee, KF=26643097500en_US

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