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Article: Generation and characterization of hammerhead ribozymes targeting rodent metallothionein-I and -II ribonucleic acid

TitleGeneration and characterization of hammerhead ribozymes targeting rodent metallothionein-I and -II ribonucleic acid
Authors
Issue Date1999
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap
Citation
Toxicology And Applied Pharmacology, 1999, v. 161 n. 3, p. 294-301 How to Cite?
AbstractTwo trans-acting hammerhead ribozymes (Rzs), Rz1-2 and Rz4-9, were designed and shown to be effective in cleaving rat or mouse metallothenein (MT)-I and MT-II mRNA, respectively, at position +147/148, in a sequence- specific manner. The catalytic efficiency for Rz1-2 on rat MT-I was found to be 678 M-1s-1 and that for Rz4-9 on rat MT-II cRNA was found to be 372 M-1s-1. An expression vector, pRz(4-9/1-2), containing both Rzs linked in tandem, was constructed and used to transfect NbE-1 cells, an immortalized ventral prostate epithelial cell line. Two stable-transfected lines, NbE- 1(Rz2) and NbE-1(Rz3), expressing substantial levels of (Rz1-2/Rz4-9) and minimal levels of MT-I and MT-II transcripts were found to exhibit increased sensitivity to cadmium (Cd)-induced cytotoxicity compared to the parent line. This article is the first to report successful degradation of rodent MT mRNA in vitro and in cellulo by hammerhead Rzs.
Persistent Identifierhttp://hdl.handle.net/10722/173235
ISSN
2015 Impact Factor: 3.847
2015 SCImago Journal Rankings: 1.593
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, KFen_US
dc.contributor.authorLau, KMen_US
dc.contributor.authorHo, SMen_US
dc.date.accessioned2012-10-30T06:28:42Z-
dc.date.available2012-10-30T06:28:42Z-
dc.date.issued1999en_US
dc.identifier.citationToxicology And Applied Pharmacology, 1999, v. 161 n. 3, p. 294-301en_US
dc.identifier.issn0041-008Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/173235-
dc.description.abstractTwo trans-acting hammerhead ribozymes (Rzs), Rz1-2 and Rz4-9, were designed and shown to be effective in cleaving rat or mouse metallothenein (MT)-I and MT-II mRNA, respectively, at position +147/148, in a sequence- specific manner. The catalytic efficiency for Rz1-2 on rat MT-I was found to be 678 M-1s-1 and that for Rz4-9 on rat MT-II cRNA was found to be 372 M-1s-1. An expression vector, pRz(4-9/1-2), containing both Rzs linked in tandem, was constructed and used to transfect NbE-1 cells, an immortalized ventral prostate epithelial cell line. Two stable-transfected lines, NbE- 1(Rz2) and NbE-1(Rz3), expressing substantial levels of (Rz1-2/Rz4-9) and minimal levels of MT-I and MT-II transcripts were found to exhibit increased sensitivity to cadmium (Cd)-induced cytotoxicity compared to the parent line. This article is the first to report successful degradation of rodent MT mRNA in vitro and in cellulo by hammerhead Rzs.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taapen_US
dc.relation.ispartofToxicology and Applied Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCadmium - Toxicityen_US
dc.subject.meshCell Line, Transformeden_US
dc.subject.meshCell Survival - Drug Effectsen_US
dc.subject.meshClone Cellsen_US
dc.subject.meshDna Primers - Chemistryen_US
dc.subject.meshEpithelial Cells - Drug Effects - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMetallothionein - Biosynthesis - Geneticsen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNucleic Acid Conformationen_US
dc.subject.meshProstate - Cytology - Drug Effects - Metabolismen_US
dc.subject.meshRna, Catalytic - Chemistry - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTransfectionen_US
dc.titleGeneration and characterization of hammerhead ribozymes targeting rodent metallothionein-I and -II ribonucleic aciden_US
dc.typeArticleen_US
dc.identifier.emailLee, KF:ckflee@hku.hken_US
dc.identifier.authorityLee, KF=rp00458en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/taap.1999.8821en_US
dc.identifier.pmid10620487-
dc.identifier.scopuseid_2-s2.0-0033572640en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033572640&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume161en_US
dc.identifier.issue3en_US
dc.identifier.spage294en_US
dc.identifier.epage301en_US
dc.identifier.isiWOS:000085233400010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLee, KF=26643097500en_US
dc.identifier.scopusauthoridLau, KM=36722691500en_US
dc.identifier.scopusauthoridHo, SM=7403713823en_US

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