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- Publisher Website: 10.1006/taap.1998.8556
- Scopus: eid_2-s2.0-0032951317
- PMID: 9882588
- WOS: WOS:000078144800003
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Article: Effects of cadmium on metallothionein-I and metallothionein-II mRNA expression in rat ventral, lateral, and dorsal prostatic lobes: Quantification by competitive RT-PCR
Title | Effects of cadmium on metallothionein-I and metallothionein-II mRNA expression in rat ventral, lateral, and dorsal prostatic lobes: Quantification by competitive RT-PCR |
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Authors | |
Issue Date | 1999 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap |
Citation | Toxicology And Applied Pharmacology, 1999, v. 154 n. 1, p. 20-27 How to Cite? |
Abstract | Highly sensitive, sequence-specific competitive reverse transcriptase- polymerase chain reaction (RT-PCR) protocols were established for the detection and quantification of metallothionein (MT)-I and MT-II messages, in absolute values, in rat tissues. Detection limits for these protocols were in the range of 5 to 10 amol per μg total RNA. Levels of MT-I and MT-II transcripts in the three major prostatic lobes, kidney, and testis were measured in untreated and cadmium (Cd)-treated rats. The dorsal prostate (DP), lateral prostate (LP), kidney, and testis expressed substantial levels of MT-I and MT-II mRNA while the ventral prostate (VP) had extremely low levels of the transcripts. Cd treatment induced higher levels of MT-I and/or MT-II mRNA expression in all tissues studied with the exception of LP. In the LP, Cd treatment caused reductions of MT-I and MT-II mRNA levels. The Cd- induced levels attained in the VP following Cd exposure were still markedly lower than those found in the kidney, testis, LP, and DP of untreated animals. These findings contradict previous claims that the MT genes in rat VP are unresponsive to Cd activation. The susceptibility of VP to Cd toxicity/carcinogenicity may therefore be explained by low levels of Cd- induced expression rather than lack of induction of MTs. |
Persistent Identifier | http://hdl.handle.net/10722/173233 |
ISSN | 2023 Impact Factor: 3.3 2023 SCImago Journal Rankings: 0.788 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, KF | en_US |
dc.contributor.author | Lau, KM | en_US |
dc.contributor.author | Ho, SM | en_US |
dc.date.accessioned | 2012-10-30T06:28:41Z | - |
dc.date.available | 2012-10-30T06:28:41Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Toxicology And Applied Pharmacology, 1999, v. 154 n. 1, p. 20-27 | en_US |
dc.identifier.issn | 0041-008X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/173233 | - |
dc.description.abstract | Highly sensitive, sequence-specific competitive reverse transcriptase- polymerase chain reaction (RT-PCR) protocols were established for the detection and quantification of metallothionein (MT)-I and MT-II messages, in absolute values, in rat tissues. Detection limits for these protocols were in the range of 5 to 10 amol per μg total RNA. Levels of MT-I and MT-II transcripts in the three major prostatic lobes, kidney, and testis were measured in untreated and cadmium (Cd)-treated rats. The dorsal prostate (DP), lateral prostate (LP), kidney, and testis expressed substantial levels of MT-I and MT-II mRNA while the ventral prostate (VP) had extremely low levels of the transcripts. Cd treatment induced higher levels of MT-I and/or MT-II mRNA expression in all tissues studied with the exception of LP. In the LP, Cd treatment caused reductions of MT-I and MT-II mRNA levels. The Cd- induced levels attained in the VP following Cd exposure were still markedly lower than those found in the kidney, testis, LP, and DP of untreated animals. These findings contradict previous claims that the MT genes in rat VP are unresponsive to Cd activation. The susceptibility of VP to Cd toxicity/carcinogenicity may therefore be explained by low levels of Cd- induced expression rather than lack of induction of MTs. | en_US |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap | en_US |
dc.relation.ispartof | Toxicology and Applied Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Binding, Competitive | en_US |
dc.subject.mesh | Cadmium - Pharmacology - Toxicity | en_US |
dc.subject.mesh | Gene Expression - Drug Effects | en_US |
dc.subject.mesh | Kidney - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Metallothionein - Genetics | en_US |
dc.subject.mesh | Prostate - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Rna, Messenger - Analysis - Metabolism | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Testis - Drug Effects - Metabolism | en_US |
dc.title | Effects of cadmium on metallothionein-I and metallothionein-II mRNA expression in rat ventral, lateral, and dorsal prostatic lobes: Quantification by competitive RT-PCR | en_US |
dc.type | Article | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1006/taap.1998.8556 | en_US |
dc.identifier.pmid | 9882588 | - |
dc.identifier.scopus | eid_2-s2.0-0032951317 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0032951317&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 154 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 20 | en_US |
dc.identifier.epage | 27 | en_US |
dc.identifier.isi | WOS:000078144800003 | - |
dc.publisher.place | United States | en_US |
dc.identifier.issnl | 0041-008X | - |