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Article: Effects of cadmium on metallothionein-I and metallothionein-II mRNA expression in rat ventral, lateral, and dorsal prostatic lobes: Quantification by competitive RT-PCR

TitleEffects of cadmium on metallothionein-I and metallothionein-II mRNA expression in rat ventral, lateral, and dorsal prostatic lobes: Quantification by competitive RT-PCR
Authors
Issue Date1999
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap
Citation
Toxicology And Applied Pharmacology, 1999, v. 154 n. 1, p. 20-27 How to Cite?
AbstractHighly sensitive, sequence-specific competitive reverse transcriptase- polymerase chain reaction (RT-PCR) protocols were established for the detection and quantification of metallothionein (MT)-I and MT-II messages, in absolute values, in rat tissues. Detection limits for these protocols were in the range of 5 to 10 amol per μg total RNA. Levels of MT-I and MT-II transcripts in the three major prostatic lobes, kidney, and testis were measured in untreated and cadmium (Cd)-treated rats. The dorsal prostate (DP), lateral prostate (LP), kidney, and testis expressed substantial levels of MT-I and MT-II mRNA while the ventral prostate (VP) had extremely low levels of the transcripts. Cd treatment induced higher levels of MT-I and/or MT-II mRNA expression in all tissues studied with the exception of LP. In the LP, Cd treatment caused reductions of MT-I and MT-II mRNA levels. The Cd- induced levels attained in the VP following Cd exposure were still markedly lower than those found in the kidney, testis, LP, and DP of untreated animals. These findings contradict previous claims that the MT genes in rat VP are unresponsive to Cd activation. The susceptibility of VP to Cd toxicity/carcinogenicity may therefore be explained by low levels of Cd- induced expression rather than lack of induction of MTs.
Persistent Identifierhttp://hdl.handle.net/10722/173233
ISSN
2015 Impact Factor: 3.847
2015 SCImago Journal Rankings: 1.593
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, KFen_US
dc.contributor.authorLau, KMen_US
dc.contributor.authorHo, SMen_US
dc.date.accessioned2012-10-30T06:28:41Z-
dc.date.available2012-10-30T06:28:41Z-
dc.date.issued1999en_US
dc.identifier.citationToxicology And Applied Pharmacology, 1999, v. 154 n. 1, p. 20-27en_US
dc.identifier.issn0041-008Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/173233-
dc.description.abstractHighly sensitive, sequence-specific competitive reverse transcriptase- polymerase chain reaction (RT-PCR) protocols were established for the detection and quantification of metallothionein (MT)-I and MT-II messages, in absolute values, in rat tissues. Detection limits for these protocols were in the range of 5 to 10 amol per μg total RNA. Levels of MT-I and MT-II transcripts in the three major prostatic lobes, kidney, and testis were measured in untreated and cadmium (Cd)-treated rats. The dorsal prostate (DP), lateral prostate (LP), kidney, and testis expressed substantial levels of MT-I and MT-II mRNA while the ventral prostate (VP) had extremely low levels of the transcripts. Cd treatment induced higher levels of MT-I and/or MT-II mRNA expression in all tissues studied with the exception of LP. In the LP, Cd treatment caused reductions of MT-I and MT-II mRNA levels. The Cd- induced levels attained in the VP following Cd exposure were still markedly lower than those found in the kidney, testis, LP, and DP of untreated animals. These findings contradict previous claims that the MT genes in rat VP are unresponsive to Cd activation. The susceptibility of VP to Cd toxicity/carcinogenicity may therefore be explained by low levels of Cd- induced expression rather than lack of induction of MTs.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taapen_US
dc.relation.ispartofToxicology and Applied Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshCadmium - Pharmacology - Toxicityen_US
dc.subject.meshGene Expression - Drug Effectsen_US
dc.subject.meshKidney - Drug Effects - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMetallothionein - Geneticsen_US
dc.subject.meshProstate - Drug Effects - Metabolismen_US
dc.subject.meshRna, Messenger - Analysis - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshTestis - Drug Effects - Metabolismen_US
dc.titleEffects of cadmium on metallothionein-I and metallothionein-II mRNA expression in rat ventral, lateral, and dorsal prostatic lobes: Quantification by competitive RT-PCRen_US
dc.typeArticleen_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/taap.1998.8556en_US
dc.identifier.pmid9882588-
dc.identifier.scopuseid_2-s2.0-0032951317en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032951317&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume154en_US
dc.identifier.issue1en_US
dc.identifier.spage20en_US
dc.identifier.epage27en_US
dc.identifier.isiWOS:000078144800003-
dc.publisher.placeUnited Statesen_US

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