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Article: Effects of human oviductal cell coculture on various functional parameters of human spermatozoa

TitleEffects of human oviductal cell coculture on various functional parameters of human spermatozoa
Authors
Issue Date1999
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 1999, v. 71 n. 2, p. 232-239 How to Cite?
AbstractObjective: To investigate the effect of human oviductal cells on various sperm functions in vitro. Design: Controlled experimental laboratory study. Setting: University gynecology unit. Patient(s): Women undergoing tubal ligation or hysterectomy and men who were visiting our subfertility clinics. Intervention(s): Coculture of oviductal cells with human spermatozoa in vitro; sperm functions were determined after coculture. Main Outcome Measure(s): Capacitation, acrosome reaction, zona binding, and oocyte fusion. Result(s): Oviductal cells and conditioned medium induced more spermatozoa to capacitate than did control medium. Although there was no difference in the spontaneous acrosome reaction between any of the groups, the coculture group had a lower percentage of acrosome-reacted spermatozoa after calcium ionophore challenge than did the control and conditioned medium groups. Coculture and conditioned medium treatment reduced the number of spermatozoa bound to the zona pellucida. The penetration rate and penetration index of the control spermatozoa in the zona-free hamster oocyte penetration test were significantly higher than that of the cocultured or conditioned medium- treated spermatozoa. Conclusion(s): Human oviductal cells promoted capacitation, stabilized the acrosome, and suppressed binding to the zona pellucida and fusion with the oocyte in vitro.
Persistent Identifierhttp://hdl.handle.net/10722/173232
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYao, YQen_US
dc.contributor.authorHo, PCen_US
dc.contributor.authorYeung, WSBen_US
dc.date.accessioned2012-10-30T06:28:40Z-
dc.date.available2012-10-30T06:28:40Z-
dc.date.issued1999en_US
dc.identifier.citationFertility And Sterility, 1999, v. 71 n. 2, p. 232-239en_US
dc.identifier.issn0015-0282en_US
dc.identifier.urihttp://hdl.handle.net/10722/173232-
dc.description.abstractObjective: To investigate the effect of human oviductal cells on various sperm functions in vitro. Design: Controlled experimental laboratory study. Setting: University gynecology unit. Patient(s): Women undergoing tubal ligation or hysterectomy and men who were visiting our subfertility clinics. Intervention(s): Coculture of oviductal cells with human spermatozoa in vitro; sperm functions were determined after coculture. Main Outcome Measure(s): Capacitation, acrosome reaction, zona binding, and oocyte fusion. Result(s): Oviductal cells and conditioned medium induced more spermatozoa to capacitate than did control medium. Although there was no difference in the spontaneous acrosome reaction between any of the groups, the coculture group had a lower percentage of acrosome-reacted spermatozoa after calcium ionophore challenge than did the control and conditioned medium groups. Coculture and conditioned medium treatment reduced the number of spermatozoa bound to the zona pellucida. The penetration rate and penetration index of the control spermatozoa in the zona-free hamster oocyte penetration test were significantly higher than that of the cocultured or conditioned medium- treated spermatozoa. Conclusion(s): Human oviductal cells promoted capacitation, stabilized the acrosome, and suppressed binding to the zona pellucida and fusion with the oocyte in vitro.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_US
dc.relation.ispartofFertility and Sterilityen_US
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.-
dc.subject.meshAcrosome Reactionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Fusionen_US
dc.subject.meshCoculture Techniquesen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshCulture Media, Conditioneden_US
dc.subject.meshFallopian Tubes - Cytologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshOocytes - Cytologyen_US
dc.subject.meshSperm Capacitationen_US
dc.subject.meshSperm-Ovum Interactionsen_US
dc.subject.meshZona Pellucidaen_US
dc.titleEffects of human oviductal cell coculture on various functional parameters of human spermatozoaen_US
dc.typeArticleen_US
dc.identifier.emailHo, PC:pcho@hku.hken_US
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_US
dc.identifier.authorityHo, PC=rp00325en_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0015-0282(98)00430-0en_US
dc.identifier.pmid9988390-
dc.identifier.scopuseid_2-s2.0-0032908291en_US
dc.identifier.hkuros42298-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032908291&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume71en_US
dc.identifier.issue2en_US
dc.identifier.spage232en_US
dc.identifier.epage239en_US
dc.identifier.isiWOS:000078393900005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYao, YQ=7403567431en_US
dc.identifier.scopusauthoridHo, PC=7402211440en_US
dc.identifier.scopusauthoridYeung, WSB=7102370745en_US

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